转IGF-1基因超细毛羊与非转基因羊肠道粪便菌群多样性及组成差异分析

doi:10.11843/j.issn.0366-6964.2020.10.007

Abstract

Abstract: The study aimed to explore the intestinal microbial community changes after transfering the IGF-1 gene into the superfine wool sheep, which would lead to potential problems in the biosafety of transgenic sheep. Forty one individuals in IGF-1 transgene positive group (GP group, female(GPF) 24 and male(GPM) 17), 43 individuals in genetically modified negative group (GN group, female(GNF) 25 and male(GNM) 18) and 29 individuals in non-genetically modified group(NG group, female(NGF) 18 and male(NGM) 11) from clinical healthy sheep in the same field were randomly selected. The rectal fecal samples were collected. The Ⅱlumina HiSeq high-throughput sequencing technology was used to determine bacterial 16S rRNA V3-V4 area sequence, and the bacteria community composition in fecal samples of transgenic superfine wool sheep and non-transgenic sheep was analyzed. There were 17 phyla, 32 classes, 56 orders, 94 families, 228 genera and 185 species. LEfSe analysis showed that there were 10 biomarkers in the GPF group, there were 5 biomarkers in GPM group, all of which were common colonization bacteria of ruminant intestinal flora. The biomarkers in NGF group was Bacteroides BS11 family, the biomarkers in NGM group was Firmicutes. They were the main dominant bacteria of ruminant intestinal flora. Analysis of shared bacteria of intestinal flora in 3 groups showed that the proportion of shared bacteria was as high as 91%-94% in 6 taxa of genus and family. It was confirmed that the introduction of IGF-1 gene did not change the overall distribution of intestinal flora in superfine wool sheep, and the transgenic sheep were biosafety and environmental safety.

Key words: IGF-1 gene, superfine wool sheep, gut microbiota structure, bacterial diversity, high-throughput sequencing, genetically modified organisms(GMO) safety

CLC Number:

S852.6
S826

WANG Xinhua, ZHANG Xingxing, WANG Limin, HUANG Xin, HAN Mengli, ZHANG Yiyuan, GUO Yanhua, TANG Hong, HE Yanhua, ZHONG Fagang, ZHOU Ping. Differences of the Intestinal Microbial Flora Diversity and Composition in IGF-1 Transgenic Superfine Wool Sheep and Non-transgenic Sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2387-2402.

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Differences of the Intestinal Microbial Flora Diversity and Composition in IGF-1 Transgenic Superfine Wool Sheep and Non-transgenic Sheep

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