山羊HABP4基因的克隆、序列分析及功能预测

doi:10.11843/j.issn.0366-6964.2021.012.010

Abstract

Abstract: The aim of this study was to clone the HABP4 gene sequence of Jianzhou goats, analyze the proteins predicted to interact with HABP4, and determine their mRNA expression, so as to identify the correlations among different genes. These data will be beneficial for exploring the role of HABP4 gene in regulating cell apoptosis in goat. Sixteen 10-month-old healthy Jianzhou goats, with the average weight of 55 kg, were randomly selected from Sichuan Jianyang DAGEDA Animal Husbandary Co. Ltd.. Nine tissue samples of heart, liver, spleen, lung, kidney, pancreas, large intestine, small intestine and rumen of each goat were quickly collected after slaughter. The total RNA was extracted by TRIzol method and reverse transcribed into cDNA. The HABP4 gene sequence was cloned by RT-PCR and sequenced for further bioinformatics analysis using online softwares. Real-time fluorescent quantitative PCR (RT-qPCR) was performed to determine the expression levels of HABP4 in different tissues (n=16), and followed by the analysis of the mRNA expression levels of 10 proteins interacted with the HABP4 protein in pancreatic tissues(n=16). Also, the correlation analysis was performed in expression among these genes. A total length of 1 496 bp HABP4 gene sequence was cloned successfully, including 61 bp of 5' UTR, 1 254 bp of CDS, and 181 bp of 3' UTR, encoding 417 amino acids. The HABP4 expressed in all detected tissues. The mRNA level of HABP4 was higher in pancreas compared with other tissues. The results of correlation analysis showed that the expression of HABP4 was significantly positively correlated with UBA52, RPL32 and RPS9. The results of functional enrichment analysis showed that HABP4 might play an important role in regulating cellular localization, metabolism, negative regulation of biological processes, translation initiation, ribosome transcript and cytoplasmic metabolism. In this study, a full length of 1 496 bp of HABP4 gene was cloned successfully, and which might be significantly correlated with UBA52, RPL32 and RPS9. This data will be beneficial for further exploring the role of HABP4 gene in regulating cell apoptosis.

Key words: goat, HABP4, clone, expression characteristics, functional prediction

CLC Number:

S858.26

WANG Xianjun, XIANG Hua, ZHANG Huanrong, REN Yupeng, ZHU Jiangjiang. Cloning, Sequence Analysis and Function Prediction of HABP4 Gene in Goat[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3426-3438.

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Cloning, Sequence Analysis and Function Prediction of HABP4 Gene in Goat

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