辅酶Q10改善LPS诱导小鼠急性肺损伤的效应分析

doi:10.11843/j.issn.0366-6964.2023.04.036

Abstract

Abstract: The purpose of this study was to investigate the protective effect and mechanism of coenzyme Q10 (CoQ10) on lipopolysaccharide (LPS)-induced acute lung injury in mice. Seventy-two healthy male C57BL/6 mice aged 8 weeks were selected and randomly divided into 6 groups:control group (CON group, 100 μL corn oil), CoQ10 intervention group (CHQ group, 50 mg·kg^-1 CoQ10), model group (LPS group, 100 μL corn oil + LPS), low-dose CoQ10 treatment group (LDQ group, 2 mg·kg^-1 CoQ10 + LPS), medium-dose CoQ10 treatment group (LMQ group, 10 mg·kg^-1 CoQ10 + LPS) and high-dose CoQ10 treatment group (LHQ group, 50 mg·kg^-1 CoQ10+LPS). The mice were continuously given different doses of CoQ10 or corn oil by gavage for 14 days, and intranasally instilled 50 μL 1 mg·mL^-1 LPS to establish an acute lung injury model. After 24 h, the mice were sacrificed, and the left lung was harvested, weighed and dried to calculate the wet-dry weight ratio (W/D) of lung tissue. The protein concentration of alveolar lavage fluid (BALF), malondialdehyde (MDA) content, reactive oxygen species (ROS) concentration, total superoxide dismutase (SOD) activity and reduced glutathione (GSH) level in lung tissue were detected. Hematoxylin and eosin (HE) staining was used to observe the pathological changes of lung tissues. TUNEL staining was used to detect the apoptosis rate of lung tissue. RT-PCR technology was used to detect the expression of mitochondrial apoptosis pathway-related genes. Western blot was used to detect the expression of MKP-1/Nrf2 pathway-related proteins in lung tissue. The results showed that compared with CON group, the W/D value and BALF protein concentration in the LPS group were significantly increased (P<0.01); lung tissue hemorrhage, alveolar wall thickening, inflammatory cell infiltration; MDA and ROS contents were significantly increased (P<0.01), SOD activity and GSH content were significantly decreased (P<0.01); TUNEL apoptosis rate and mitochondrial apoptosis pathway related gene expression were significantly increased (P<0.01); MKP-1 and Nrf2 protein expression were significantly decreased (P<0.01). Low-dose, medium-dose and high-dose CoQ10 significantly or extremely significantly reversed the changes of the above indexes induced by LPS (P<0.05, P<0.01), and high-dose CoQ10 was more effective (P<0.01). In addition, there was no significant difference in all indicators between the CHQ group and the CON group (P>0.05). The results suggest that CoQ10 can improve LPS-induced acute lung injury in mice by activating MKP-1/Nrf2 signaling pathway, enhancing anti-oxidative stress ability, reducing ROS production and inhibiting mitochondrial apoptosis.

Key words: coenzyme Q10, acute lung injury, oxidative stress, mitochondrial apoptosis, MKP-1/Nrf2 signaling pathway

CLC Number:

S857.11

CHEN Yongping, KOU Yuhong, JIAO Wenjing, HOU Xiaoyu, FAN Honggang. Effect of Coenzyme Q10 on LPS-induced Acute Lung Injury in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1730-1741.

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Effect of Coenzyme Q10 on LPS-induced Acute Lung Injury in Mice

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