右美托咪定抑制NOX4缓解急性应激致大鼠肾损伤

doi:10.11843/j.issn.0366-6964.2023.02.034

Abstract

Abstract: This study was conducted to investigate the protective effects of dexmedetomidine (DEX) on renal injury caused by acute stress in rats and to explore the protective pathways of DEX on rat kidney from the perspective of oxidative stress. In this study, an acute restraint stress model was used, in which rats were forced to swim for 15 minutes and restrained for 3 hours. This experiment was performed with biochemical assays, histopathological section observation to assess renal function, followed by determination of oxidative stress and proteins of pathways related to oxidative stress. The open-field experiments confirmed that the acute stress model had been successfully established. DEX decreased NOX4 expression and increased Nrf2/HO-1/NQO1 expression, and the kidney biochemical results were significantly normalized in the DEX-treated group compared with the acute stress group, and the damage was significantly reduced in the pathological sections. The experimental results suggest that DEX treatment of acute stress can affect NOX4/Nrf2/HO-1/NQO1 signaling pathway and inhibit oxidative stress. Therefore, DEX has a protective effect on acute stress-induced renal injury and has potential clinical applications in stress syndromes.

Key words: acute stress, oxidative stress, kidney injury, dexmedetomidine, NOX4

CLC Number:

S856.59

YANG Haotian, CHEN Yongping, WANG Zhiqiang, HUANG Yuxiang, MA Zhigang, ZOU Yue, WEI Niandong, ZHANG Hong, LI Xin, DONG Jiaqiang, LÜ Mingzhe, LI Hongbin, LIU Liwei, YANG Haoxuan, ZHANG Guohua, LIU Xuesong, ZHONG Peng, SHI Heye, KOU Yuhong, CHEN Zhifeng. Dexmedetomidine Inhibits NOX4 and Alleviates Acute Stress-induced Renal Injury in Rats[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 779-786.

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Dexmedetomidine Inhibits NOX4 and Alleviates Acute Stress-induced Renal Injury in Rats

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