基于网络药理学分析党参减轻大肠杆菌感染小鼠急性肺损伤的作用机制

doi:10.11843/j.issn.0366-6964.2023.08.040

Abstract

Abstract: The network pharmacology and related molecular biology experiments were used to explore the protective mechanism of Codonopsis pilosula on acute lung injury (ALI). The main chemical components of Codonopsis radix and related targets were mined through the network pharmacology database. The related targets of ALI were obtained through disease-related databases. protein interaction (PPI) network was constructed,and GO analysis and KEGG pathway enrichment analysis were performed on the mined data. The results showed that 8 compounds in Codonopsis pilosula could act on 303 targets, 1 522 targets related to ALI, 119 intersecting targets involved in ALI, 103 targets in PPI network including MAPK, NF-κB and TLR4. GO functional enrichment analysis showed 133 molecular functions, 79 biological processes and 523 cellular components, and KEGG showed 132 related pathways. The results were verified by Western blotting, ELISA and immunofluorescence. The results showed that Codonopsis pilosula polysaccharides pretreatment could down-regulate MAPK and NF-κB inflammatory signal pathways activation and TNF α and IL-1 β secretion in E.coli-induced macrophages. Furthermore, Codonopsis pilosula polysaccharide could down-regulate TNF α and IL-1β production, and down-regulate HMGB1 expression to reduce the lung injury in the E.coli-infected lungs of mice. In conclusion, Codonopsis pilosula could protect the lung by regulating disease-related targets of ALI through multiple pathways and biological processes.

Key words: acute lung injury, Codonopsis pilosula, network pharmacology, targets

CLC Number:

S853.74

GONG Zhiguo, ZHAO Jiamin, GU Baichen, REN Peipei, YU Zhuoya, BAI Yunjie, LIU Xinyu, WANG Chao, LIU Bo. Exploring the Mechanism of Codonopsis pilosula in Alleviation of Acute Lung Injury in Escherichia coli Infected Mice Based on Network Pharmacology[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3571-3581.

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Exploring the Mechanism of Codonopsis pilosula in Alleviation of Acute Lung Injury in Escherichia coli Infected Mice Based on Network Pharmacology

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