烟酸通过降低氧化应激水平提高绵羊精子低温保存效果

doi:10.11843/j.issn.0366-6964.2023.05.019

Abstract

Abstract: Low temperature preservation can effectively prolong the storage time of sperm in vitro and improve the semen utilization ratio. However, long-term exposure to low temperature will cause the continuous accumulation of reactive oxygen species (ROS), induce sperm oxidative stress, reduce the integrity of plasma membrane and result in sperm motility decline. This study intends to investigate the effect of adding different concentrations of niacin (nicotinic acid, NA) to the diluents on sperm preservation under the condition of 4℃ storage of sheep sperm, and to explore its mechanism of action. In this study, semen from healthy adult German Merino rams (n=6) were collected and supplemented with 5、10 and 20 mmol·L^-1 NA used as protective agents to clarify the protective effect of NA on sheep sperm. Sperm motility and motility were detected by sperm analyzer, plasma membrane integrity was analyzed by HOST,ROS level and mitochondrial function in sperm were detected by fluorescent staining, while H₂O₂ content, antioxidant factor activity level and ATP level were detected by kit. The results showed that compared with 10% BSA control group, the survival time of sperm treated with NA could be extended to 120 h,and the sperm motility could still reach 70% at 72 h after 10 mmol·L^-1NA treatment. At 24~72 h after NA treatment, sheep sperm maintained higher motility, movement characteristic and plasma membrane integrity (P<0.05), and decreased ROS levels in sperm cells (P<0.05). At 72 h after NA treatment, the content of H₂O₂in sperm was significantly lower than that in the 10% BSA control group (P<0.05), while the antioxidant factors (CAT,GSH-PX,T-SOD,T-AOC) activity levels, mitochondrial function and ATP levels were significantly better than 10% BSA control group (P<0.05). In conclusion, adding NA to sheep semen low temperature dilution under 4℃ storage conditions can improve sperm motility, movement characteristic, plasma membrane integrity, antioxidant capacity, sperm mitochondrial function and ATP. It can effectively improve the low temperature preservation effect of sheep sperm.

Key words: sheep, nicotinic acid, sperm motility, mitochondria, oxidative stress

CLC Number:

S826.34

HAN Xiuyuan, ZHAO Liang, WANG Chuang, QI Meiyu, YAO Yuchang. Nicotinic Acid Enhances Low Temperature Preservation of Sheep Sperm by Reducing Oxidative Stress Levels[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1979-1989.

References 34

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Nicotinic Acid Enhances Low Temperature Preservation of Sheep Sperm by Reducing Oxidative Stress Levels

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