壳寡糖对仔猪脑海马氧化应激的抑制作用及机制研究

doi:10.11843/j.issn.0366-6964.2023.02.031

Abstract

Abstract: Oxidative stress is closely related to the growth retardation and the occurrence and development of diseases in animals. Chito-oligosaccharide (COSs) has the effect of anti-oxidative stress, but the effect and molecular mechanism of COSs in inhibiting oxidative stress in hippocampus of brain in piglets are not clear. In this study, the oxidative stress model of piglets was established by intramuscular injection of dextran iron (FeDex), and on this basis, the effects of COSs on the average daily gain, oxidation and anti-oxidation related indexes of serum and some tissue, expression levels of apoptosis related protein gene and neuropeptide cocaine and amphetamine regulated transcript (CART) genes of hippocampus in piglets were investigated. Furthermore, the correlation between the expression levels of CART gene and apoptosis-related protein gene was analyzed. The results showed that compared with the control group, the daily gain, the levels of glutathione (GSH) and total anti-oxidant capacity (T-AOC) in serum, liver, lung, hippocampus and cerebral cortex of piglets with oxidative stress were significantly decreased (P<0.01 or P<0.05), while the contents of hydrogenperoxide (H₂O₂) and malondialdehyde (MDA) were significantly increased (P<0.01 or P<0.05). Intragastrical administration of 50 mg·kg^-1 COSs significantly increased the daily gain, the levels of GSH and T-AOC in serum, liver, lung, hippocampus and cerebral cortex (P<0.01 or P<0.05), while decreased the contents of H₂O₂ and MDA in serum and liver, lung, hippocampus and cerebral cortex of piglets with oxidative stress (P<0.01 or P<0.05). Compared with the control group, the expression levels of Cleaved Caspase-3 and Bax genes were significantly up-regulated in hippocampus of piglets with oxidative stress (P<0.05), while the expression level of Bcl-2 gene was significantly down-regulated (P<0.05). COSs significantly decreased the expression levels of Bax and Cleaved Caspase-3 genes in the hippocampus of piglets with oxidative stress (P<0.05), while significantly increased the expression level of Bcl-2 (P<0.05). Further study showed that compared with the control group, the expression levels of CART gene were significantly decreased in piglets with oxidative stress (P<0.01), while COSs significantly increased the expression levels of CART gene (P<0.01). The results of correlation analysis showed that CART was significantly correlated with the expression level of apoptosis-related protein genes. COSs can inhibit oxidative stress of hippocampus of piglets by weakening apoptosis signal pathway, which may be mediated by neuropeptide CART.

Key words: chitooligosaccharides, piglets, oxidative stress, apoptosis, neuropeptide-CART

CLC Number:

S857.3

YANG Chengying, WANG Kai, HUANG Ziqing, LIN Hailan, WANG Naixiu, LI Yuhang, LIU Yanqing, LIU Yuxuan, ZHU Yan, HE Daoling, CHEN Hongyue, GAN Ling. Study on the Inhibitory Effect of Chito-oligosaccharide on Oxidative Stress inHippocampus of Piglets and Its Mechanism[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 744-756.

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Study on the Inhibitory Effect of Chito-oligosaccharide on Oxidative Stress inHippocampus of Piglets and Its Mechanism

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