猪流行性腹泻病毒全病毒灭活疫苗量效关系研究

doi:10.11843/j.issn.0366-6964.2022.05.021

Abstract

Abstract: This study aims to investigate the protective effect of infected piglets which were immunized with different dose of inactivated porcine epidemic diarrhea virus (PEDV) vaccines. The number of infective virus particles and total virus particles of PEDV with different concentrations were determined, and the mice were immunized with different concentration vaccine prepared as antigen, respectively. The humoral and cellular immune production were determined by ELISA antibody detection method, neutralization test and ELISPOT method. Vaccine with appropriate antigen content was selected to immunize piglets, then the antibody was determined. The relationship between concentrated vaccine and protective effect was studied by challenge experiment. The results showed that, when the antigen dose was equal or greater than 8×10⁶ pfu·mL^-1, the inactive vaccine could effectively stimulate mice to produce humoral and cellular immunity. The piglets immunized with 2 mL inactivated PEDV vaccine containing 8×10⁶ pfu·mL^-1 antigen could resist diarrhea and continuous viral shedding caused by PEDV challenge. Compared with the total number of virus particles, the number of infectious virus particles was significantly correlated with antibody production (r=0.998 1), and neutralization titer was significantly correlated with piglet protection (r=0.974 7). PEDV inactivated vaccine can provide good immune protection, in which the number of infectious virus particles is the key factor to improve the antibody level. Antibody titer, as an index of humoral immunity, is an important reference for judging immune protection.

Key words: porcine epidemic diarrhea virus, inactivated vaccine, dose-titration

CLC Number:

S855.3

HAO Jianwei, XUE Chunyi, CAO Yongchang. The Dose-titration Study of Inactivated Vaccine of Porcine Epidemic Diarrhea Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1536-1543.

References 23

[1]	MASTERS P S,PERLMAN S.Coronaviridae[M]//KNIPE D M,HOWLEY P M.Fields Virology.Philadelphia:Wolters Kluwer,2013:825.
[2]	WOOD E N.An apparently new syndrome of porcine epidemic diarrhoea[J].Vet Rec,1977,100(12):243-244.
[3]	SONG D,PARK B.Porcine epidemic diarrhoea virus:a comprehensive review of molecular epidemiology,diagnosis,and vaccines[J].Virus Genes,2012,44(2):167-175.
[4]	WON H,LIM J,NOH Y H,et al.Efficacy of porcine epidemic diarrhea vaccines:a systematic review and meta-analysis[J].Vaccines,2020,8(4):642.
[5]	SONG D,MOON H,KANG B.Porcine epidemic diarrhea:a review of current epidemiology and available vaccines[J].Clin Exp Vaccine Res,2015,4(2):166-176.
[6]	SATO T,TAKEYAMA N,KATSUMATA A,et al.Mutations in the spike gene of porcine epidemic diarrhea virus associated with growth adaptation in vitro and attenuation of virulence in vivo[J].Virus Genes,2011,43(1):72-78.
[7]	THAN V T,CHOE S E,VU T T H,et al.Genetic characterization of the spike gene of porcine epidemic diarrhea viruses (PEDVs) circulating in Vietnam from 2015 to 2016[J].Vet Med Sci,2020,6(3):535-542.
[8]	COLLIN E A,ANBALAGAN S,OKDA F,et al.An inactivated vaccine made from a U.S. field isolate of porcine epidemic disease virus is immunogenic in pigs as demonstrated by a dose-titration[J].BMC Vet Res,2015,11(1):62.
[9]	CAVANAGH D.Severe acute respiratory syndrome vaccine development:experiences of vaccination against avian infectious bronchitis coronavirus[J].Avian Pathol,2003,32(6):567-582.
[10]	FLECK R.疫苗诱导对猪肺炎支原体的免疫防卫[J].汪秉刚,译.国外畜牧学(猪与禽),2002(5):44-46.FLECK R.Vaccination-induced immune defense against M. Hyo[J].WANG B G,trans.Animal Sci Abroad-Pigs Poultry,2002(5):44-46.(in Chinese)
[11]	HAO J W,XUE C Y,HE L L,et al.Bioinformatics insight into the spike glycoprotein gene of field porcine epidemic diarrhea strains during 2011-2013 in Guangdong,China[J].Virus Genes,2014,49(1):58-67.
[12]	ZHANG X B,HAO J W,ZHEN J,et al.Rapid quantitation of porcine epidemic diarrhea virus (PEDV) by Virus Counter[J].J Virol Methods,2015,223:1-4.
[13]	HAO J W,ZHANG Y,FANG S K,et al.Evaluation of purified recombinant spike fragments for assessment of the presence of serum neutralizing antibodies against a variant strain of porcine epidemic diarrhea virus[J].Virol Sin,2017,32(4):307-316.
[14]	GANGES L,CROOKE H R,BOHóRQUEZ J A,et al.Classical swine fever virus:the past,present and future[J].Virus Res,2020,289:198151.
[15]	WANG J,CUI X,WANG X B,et al.Efficacy of the Bartha-K61 vaccine and a gE-/gI-/TK- prototype vaccine against variant porcine pseudorabies virus (vPRV) in piglets with sublethal challenge of vPRV[J].Res Vet Sci,2020,128:16-23.
[16]	WANG D,FANG L R,XIAO S B.Porcine epidemic diarrhea in China[J].Virus Res,2016,226:7-13.
[17]	GERDTS V,ZAKHARTCHOUK A.Vaccines for porcine epidemic diarrhea virus and other swine coronaviruses[J].Vet Microbiol,2017,206:45-51.
[18]	MOGLER M A,GANDER J,HARRIS D L.Development of an alphavirus RNA particle vaccine against porcine epidemic diarrhea virus[J].Ann Proc Amer Assn Swine Vets,2014,63-64.
[19]	GOEDE D,MURTAUGH M P,NEREM J,et al.Previous infection of sows with a "mild" strain of porcine epidemic diarrhea virus confers protection against infection with a "severe" strain[J].Vet Microbiol,2015,176(1-2):161-164.
[20]	WU P,RODRIGUEZ Y Y,HERSHEY B J,et al.Validation of a binary ethylenimine (BEI) inactivation procedure for biosafety treatment of foot-and-mouth disease viruses (FMDV),vesicular stomatitis viruses (VSV),and swine vesicular disease virus (SVDV)[J].Vet Microbiol,2021,252:108928.
[21]	SONG D S,OH J S,KANG B K,et al.Oral efficacy of Vero cell attenuated porcine epidemic diarrhea virus DR13 strain[J].Res Vet Sci,2007,82(1):134-140.
[22]	BANDRICK M,ARIZA-NIETO C,BAIDOO S K,et al.Colostral antibody-mediated and cell-mediated immunity contributes to innate and antigen-specific immunity in piglets[J].Dev Comp Immunol,2014,43(1):114-120.
[23]	JUNG K,SAIF L J,WANG Q H.Porcine epidemic diarrhea virus (PEDV):an update on etiology,transmission,pathogenesis,and prevention and control[J].Virus Res,2020,286:198045.

The Dose-titration Study of Inactivated Vaccine of Porcine Epidemic Diarrhea Virus

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 23

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Jing, ZHANG Shujuan, HU Xia, LIU Xiangyang, ZHANG Xingcui, SONG Zhenhui. CD44 Regulates Na⁺/H⁺ Exchanger 3 Activity by Influencing Porcine Epidemic Diarrhea Virus Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2176-2185.
[2]	HU Zeqi, LI Runcheng, TAN Zuming, XIE Xiuyan, WANG Jiangping, QIN Lejuan, LI Rong, GE Meng. Establishment and Preliminary Application of PEDV, PoRVA and PDCoV TaqMan Triple RT-qPCR Assay [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2267-2272.
[3]	LIN Lili, ZHANG Mengdi, ZHU Linlin, MA Hailong, SUN Qi, HE Qigai, ZHANG Mengjia, LI Wentao. Establishment of Neutralizing Antibody Detection Method based on Recombinant Fluorescent Virus of Porcine Epidemic Diarrhea Virus GⅡb Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1649-1660.
[4]	REN Lixin, ZHANG Jingyi, XU Shasha, YANG Liu, ZHANG Xingcui, SONG Zhenhui. Effect of ACE2 on Porcine Intestinal Epithelial Cells Infected with Porcine Epidemic Diarrhea Virus in vitro [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1238-1248.
[5]	ZHOU Jianhao, WANG Dongfang, LIU Ying, WANG Shujuan, MA Zhenyuan, XIE Caihua, ZHAO Xueli, YANG Haibo, FENG Guidan, KANG Taisheng, HU Yufeng, LI Bowen, YAN Ruoqian. Establishment and Preliminary Application of a Quantitative Droplet Digital PCR Assay for Porcine Epidemic Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 413-418.
[6]	LIU Hua, YIN Dongdong, SHAO Ying, SONG Xiangjun, WANG Zhenyu, PAN Xiaocheng, TU Jian, HE Changsheng, ZHU Liangqiang, QI Kezong. Detection of Porcine Epidemic Diarrhea Virus by Recombinase Aided Amplification Combined with CRISPR/Cas13a [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3991-3997.
[7]	ZHANG Senhao, WANG Xueying, CAI Limeng, XIE Weichun, KUANG Hongdi, WANG Xiaona, LI Jiaxuan, CUI Wen, JIANG Yanping, ZHOU Han, SHAN Zhifu, WANG Li, QIAO Xinyuan, LI Yijing, TANG Lijie. Establishment and Application of Rapid Diagnosis Method for RPA of Porcine Epidemic Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2498-2508.
[8]	ZHAI Gang, GU Wenyuan, LIU Tao, LIU Ying, ZHANG Shuai, FAN Jinghui, ZUO Yuzhu. Establishment of TaqMan Detection Method of Porcine Epidemic Diarrhea Virus and Analysis of Genetic Variation based on S Gene [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 847-854.
[9]	MENG Lingzhai, CHEN Chunli, YU Mengmeng, WANG Zhanxin, WANG Suyan, LIU Peng, HE Tana, GUO Ru, CHEN Yuntong, LIU Changjun, QI Xiaole, WU Zhiqiang, GAO Yulong. Pathogenicity of Avian Metapneumovirus Subtype B on Yellow Feather Broilers and Evaluation on Immune Effect of Inactivated Vaccine [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5154-5161.
[10]	HE Chenxiang, GAO Shandian, TIAN Zhancheng, DU Junzheng, WANG Jinming, GUAN Guiquan, YIN Hong. Development of a Differential Diagnostic ELISA Based on G_NS Protein to Distinguish BEFV Infected and Vaccinated Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4320-4326.
[11]	WANG Tianyu, LI Zhiwei, YANG Ting, DONG Linfang, MA Zhiqian, BIANBA Ciren, XIAO Shuqi, LI Shuang. Screening and Identification of Nanobodies against Porcine Epidemic Diarrhea Virus S Protein [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2589-2598.
[12]	ZHENG Hongqing, WU Xujin, ZHU Xiaofu, YIN Baoying, GAO Junhua, LI Yanzhi, ZHI Chanping. Porcine Epidemic Diarrhea Virus Regulates Cell Apoptosis by miR-133c-3p/BCL2L2 Axis [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2233-2243.
[13]	ZHANG Zhicheng, CHEN Meijuan, AI Jun. Bayesian Inference and Simulation on True Prevalence of Porcine Epidemic Diarrhea Virus in American Swine Population [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(7): 2013-2024.
[14]	LIU Ruyue, FAN Jinghui, LIU Tao, YUAN Junhui, LI Qingyan, ZHAI Xianghe, ZUO Yuzhu. Construction and Identification of Hepatitis B Core Antigen Virus-like Particles Carrying PEDV S1 Epitope [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1760-1764.
[15]	SUN Pu, HE Wei, FU Yuanfang, LI Dong, YANG Lin, WEI Delong, CAO Yimei, LI Pinghua, BAI Xingwen, MA Xueqing, LI Kun, BAO Huifang, ZHANG Jing, ZHU Xinrong, LIU Zaixin, LU Zengjun. Immune Effect of Inactivated Vaccines against Foot-and-Mouth Disease and Their Interference on Differential Diagnosis [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2481-2489.