携带猪流行性腹泻病毒S1抗原表位的乙肝核心抗原病毒样颗粒的构建与鉴定

doi:10.11843/j.issn.0366-6964.2021.06.031

Abstract

Abstract: A study was undertaken to construct a virus-like particle that uses hepatitis B core antigen (HBcAg) as a carrier to present PEDV S1 epitope. In this work, the 270 bp gene containing the B cell epitope in the PEDV S1 protein was inserted into the main immunodominant region (MIR) of the HBcAg to construct the recombinant plasmid pET-32a(+)-HBcAg-PEDV S1 and transform it into competent cell BL21 (DE3), the expression was induced by IPTG, and the expressed protein was purified with Ni column. The purified recombinant protein was identified by SDS-PAGE, and its morphology was detected by transmission electron microscope. The recombinant plasmid pET-32a(+)-HBcAg-PEDV S1 was successfully constructed and expressed as inclusion bodies in BL21(DE3). The purified and renatured recombinant protein, virus-like particle structure was detected by transmission electron microscope after 2% phosphotungstic acid negative staining. HBcAg-PEDV S1 recombinant protein can spontaneously form a virus-like particle structure. In prokaryotic expression, HBcAg can be used as a carrier to present PEDV S1 epitope, which provides ideas for future research on new PED vaccines.

Key words: porcine epidemic diarrhea virus, S1 epitope, hepatitis B core antigen, virus-like particles, prokaryotic expression

CLC Number:

S852.659.6

LIU Ruyue, FAN Jinghui, LIU Tao, YUAN Junhui, LI Qingyan, ZHAI Xianghe, ZUO Yuzhu. Construction and Identification of Hepatitis B Core Antigen Virus-like Particles Carrying PEDV S1 Epitope[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1760-1764.

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Construction and Identification of Hepatitis B Core Antigen Virus-like Particles Carrying PEDV S1 Epitope

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