母猪妊娠早期血液中差异表达蛋白的鉴定

doi:10.11843/j.issn.0366-6964.2022.04.011

Abstract

Abstract: The study aimed to investigate the proteins expression in peripheral blood at early pregnancy, and to discover potential protein biomarkers for early pregnancy diagnosis in sows. In this study, the peripheral blood from 8 healthy, normal production performance and similar physical condition of pregnant and non-pregnant Landrace×Large White multiparous sows was collected on the 15th day after mating. Proteins were isolated, then iTRAQ technique and bioinformatics software were employed to screen differentially expressed proteins related to embryo attachment. ELISA was used to verify its expression and ROC curve was analyzed. A total of 24 differentially expressed proteins were screened in the peripheral blood of pregnant and non-pregnant sows(19 were up-regulated and 5 were down-regulated in pregnant sows), which mainly enriched in PI3K-Akt, p53, MAPK, Estrogen, mTOR and other important signaling pathways. ELISA test verified their expression trend were consistent with iTRAQ results, which proved the accuracy of iTRAQ results. ROC curve analysis showed that FLNC, ADIPOQ, HP, TERF2, FLNC-ADIPOQ and ADIPOQ-HP could be used as a potential biomarker for early pregnancy of sows, with good accuracy and specificity. These results suggest that the differential proteins FLNC, ADIPOQ, HP and TERF2 can be used as potential biomarkers for early pregnancy diagnosis in sows, and provide a new view for the study of the mechanism of embryos implantation in sows.

Key words: iTRAQ, proteomics, sows, early pregnancy, biomarkers

CLC Number:

S828.3

QI Mengfan, XIE Su, GAO Ruonan, SUN Yishan, SUN Xiaomei, HE Junfei, LU Huiwen, LU Shihao, CHEN Xin, LI Qingchun, HUANG Tao. Identification of Differentially Expressed Proteins in Blood of Sows at Early Pregnancy[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1109-1121.

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Identification of Differentially Expressed Proteins in Blood of Sows at Early Pregnancy

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