基于蛋白组学体外分析益母草水煎液对补体和凝血级联通路相关活性因子的作用

doi:10.11843/j.issn.0366-6964.2022.06.027

Abstract

Abstract: The present study was aimed to investigate the regulatory effect of Leonurus artemisia decoction on the expression of coagulation and anticoagulation cytokines in human dermal microvascular endothelial cells (HDMECs) by proteomics. The safe concentration of Leonurus artemisia decoction to HDMECs was evaluated by MTT method. The changes of protein expression of HDMECs treated with 50 and 100 μg·mL^-1 Leonurus artemisia decoction for 24 h were analyzed by isobaric tags for relative and absolute quantification (iTRAQ) technology. By comparing the changes of protein profiles in each group, the significantly different related pathways were screened out, and the credible differentially expressed proteins (DEPs) screened in the corresponding pathway were analyzed. The antagonistic factors related to coagulation and anticoagulation in credible DEPs were selected for RT-PCR and ELISA validation. Results showed that Leonurus artemisia decoction less than 1 mg·mL^-1 had no toxic effect on HDMECs. Leonurus artemisia decoction could regulate both platelet activation related to coagulation, and heparin binding related to anticoagulation. The analysis of five credible DEPs in the screened complement and coagulation cascade pathways showed that compared with the control group, the levels of prothrombin (F2), antithrombin-Ⅲ (AT-Ⅲ), tissue plasminogen activator (t-PA), coagulation factor Ⅴ (F5) and kininogen (KNG) were significantly decreased in 50 μg·mL^-1 Leonurus artemisia decoction group. The levels of F2, AT-Ⅲ, t-PA, KNG were significantly decreased in 100 μg·mL^-1 Leonurus artemisia decoction group. Compared with 50 μg·mL^-1 Leonurus artemisia decoction group, the levels of F2, AT-Ⅲ, t-PA, KNG and F5 were significantly increased in 100 μg·mL^-1 Leonurus artemisia decoction group. This study indicate that Leonurus artemisia decoction incubation could significantly change the protein expression profile of HDMECs, and bidirectionally regulate antagonistic factors related to coagulation and anticoagulation by regulating the expression of complement and coagulation cascade-related factors F2, AT-Ⅲ, t-PA, F5 and KNG.

Key words: Leonurus artemisia decoction, HDMECs, iTRAQ, complement and coagulation cascade pathway, coagulation, anticoagulation

CLC Number:

S853.75

ZHANG Meng, WANG Weiran, FENG Chen, ZHANG Yi, ZHANG Qian, MU Xiang. The Effect of Leonurus artemisia Decoction on the Active Factors of Complement and Coagulation Cascade Pathway Based on Proteomics in vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1934-1944.

References 25

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The Effect of Leonurus artemisia Decoction on the Active Factors of Complement and Coagulation Cascade Pathway Based on Proteomics in vitro

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