家畜早期妊娠诊断的研究进展

doi:10.11843/j.issn.0366-6964.2023.05.002

Abstract

Abstract: This article reviewed the clinical diagnostics, laboratory diagnostics and circulating cell-free fetal DNA(ccffDNA) detection of pregnancy from the following four aspects:principle, method, diagnostic time and accuracy. Through the comparison and comprehensive analysis of the above methods, The advantages, limitations, research trends and the actual application effects of the above diagnostic methods were summarized, in order to provide suitable methods of livestock pregnancy diagnosis applied in different situations. The traditional clinical diagnostics have the double advantages of fast and cheap, but the accuracy is susceptible to the artificial operation. Laboratory diagnostics are earlier and more accurate, but they have disadvantages of lacking unified standard and costing more. The detection of ccffDNA can be used to identify fetal sex in domestic animals, whereas the specific markers of non-sex-dependent fetal DNA are still needed to be explored further.

Key words: radioimmunoassay, enzyme immunoassay, early pregnancy factor, pregnancy-associated glycoproteins, circulating cell-free fetal DNA

CLC Number:

S814.8

LI Wenqiao, LI Fadi, WANG Xinji, YUE Xiangpeng. Advance of Early Pregnancy Diagnosis in Livestock[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1782-1791.

References 60

[1]	郭腾龙,汝振远,任尚,等.牛早期妊娠诊断的研究进展[J].畜牧与兽医,2021,53(8):142-146.GUO T L,RU Z Y,REN S,et al.Progress in research on early pregnancy diagnosis in cattle[J]. Animal Husbandry & Veterinary Medicine,2021,53(8):142-146.(in Chinese)
[2]	菅原七郎.家畜繁殖生理の研究動向とその応用42[J].畜産の研究,1985,39(6):807-813.JIANYUAN Y L.Research trends and applications of reproductive physiology of livestock[J]. Research on Livestock Production,1985,39(6):807-813.(in Japanese).
[3]	于农淇,梁莎莎,黄健,等.阴道分泌物在动物发情鉴定中的研究进展[J].中国牛业科学,2022,48(5):66-70.YU N Q,LIANG S S,HUANG J,et al.Research progress of vaginal secretions in animal estrus identification[J]. China Cattle Science,2022,48(5):66-70.(in Chinese)
[4]	李瑞甫,杨家民.猪场常用的繁殖技术[J].养殖与饲料,2019(9):38-39.LI R F,YANG J M.Breeding techniques commonly used in pig farms[J]. Animals Breeding and Feed,2019(9):38-39.(in Chinese)
[5]	屈宏莉.羊秋季配种繁育管理要点[J].畜牧兽医科学:电子版,2020(12):86-87.QU H L.Main points of breeding management of sheep in autumn[J]. Graziery Veterinary Sciences:Electronic Version,2020(12):86-87.(in Chinese)
[6]	努尔兰·木合买提,赛力克·库鲁西,努尔别克·哈木巴太,等.母马妊娠诊断的方法[J].新疆畜牧业,2016(1):63.MUHEMAITI N R L,KULUXI S L K,HAMUBADA N R B K,et al.Methods of pregnancy diagnosis in mares[J]. Xinjiang Journal of Animal Industry,2016(1):63.(in Chinese)
[7]	李德远,邹雪伟.试情法在猪早期妊娠诊断中的应用研究[C]//河南省畜牧兽医学会第七届理事会第二次会议暨2008年学术研讨会论文集.郑州:河南省科学技术协会,2008:39-45.LI D Y,ZOU X W.Study on use of the teasing methods for pregnancy diagnosis of the early gestation stage in sow[C]//The Second Meeting of the Seventh Council of Henan Animal Husbandry and Veterinary Society and the Proceedings of the 2008 Symposium.Zhengzhou:Henan Association of Science and Technology,2008:39-45.(in Chinese)
[8]	申子平.猪妊娠诊断技术概述[J].湖南畜牧兽医,2018(6):15-17.SHEN Z P.Overview of pregnancy diagnostic techniques in sow[J]. Hunan Journal of Animal Science & Veterinary,2018(6):15-17.(in Chinese)
[9]	才巴特.牛妊娠诊断技术现状及推广[J].中国畜牧业,2022(10):112-113.CAI B T.Present situation and popularization of pregnancy diagnosis techniques in cattle[J]. China Animal Industry,2022(10):112-113.(in Chinese)
[10]	苏银池,薛永康,张震,等.奶牛场常用早期妊娠诊断技术研究进展[J].中国奶牛,2021(10):12-15.SU Y C,XUE Y K,ZHANG Z,et al.Research progress of early pregnancy diagnosis techniques commonly used in large-scale dairy farms[J]. China Dairy Cattle,2021(10):12-15.(in Chinese)
[11]	左海洋,陈晓丽,蔡勇,等.奶牛早期妊娠诊断技术研究进展[J].畜牧兽医学报,2014,45(10):1584-1591.ZUO H Y,CHEN X L,CAI Y,et al.Advance of early pregnancy diagnosis technology in dairy cows[J]. Acta Veterinaria et Zootechnica Sinica,2014,45(10):1584-1591.(in Chinese)
[12]	FRICKE P M,RICCI A,GIORDANO J O,et al.Methods for and implementation of pregnancy diagnosis in dairy cows[J]. Vet Clin North Am Food Anim Pract,2016,32(1):165-180.
[13]	陈宁.绒山羊妊娠诊断技术[J].当代畜禽养殖业,2020(6):54-55.CHEN N.Pregnancy diagnostic technique of down producing goat[J]. Modern Animal Husbandry,2020(6):54-55.(in Chinese)
[14]	练志全,邓铭,孙宝丽,等.羊的早期妊娠诊断及产羔数预测研究进展[J].家畜生态学报,2020,41(3):86-89.LIAN Z Q,DENG M,SUN B L,et al.Research progress on diagnosis of early pregnancy and prediction of litter size of ewes[J]. Acta Ecologae Animalis Domastici,2020,41(3):86-89.(in Chinese)
[15]	岳立,李秀菊,吴风光.母牛早期妊娠诊断技术[J].中国畜禽种业,2020,16(3):83.YUE L,LI X J,WU F G.Early pregnancy diagnostic techniques in cows[J]. The Chinese Livestock and Poultry Breeding,2020, 16(3):83.(in Chinese)
[16]	刘瑞生.超声波技术在母猪妊娠诊断上的研究概况[J].养猪,2018(2):53-56.LIU R S.Overview of the research on ultrasonic technique in sow pregnancy diagnosis[J]. Swine Production,2018(2):53-56.(in Chinese)
[17]	王秀娜,王大力,高安崇,等.B型超声诊断仪在猪生产学实践教学中的应用[J].畜牧兽医杂志,2021,40(4):77-80.WANG X N,WANG D L,GAO A C,et al.Application of B mode ultrasound diagnostic equipment in practical teaching of swine production[J]. Journal of Animal Science and Veterinary Medicine,2021,40(4):77-80.(in Chinese)
[18]	GONZALEZ-BULNES A,PALLARES P,VAZQUEZ M I.Ultrasonographic imaging in small ruminant reproduction[J]. Reprod Domest Anim,2010,45(S2):9-20.
[19]	李福慧.规模化奶牛场B超早期妊娠诊断规程的研究[D].呼和浩特:内蒙古农业大学,2021.LI F H.Research on early pregnancy diagnosis procedure by ultrasonography in scaled dairy farm[D].Hohhot:Inner Mongolia Agricultural University,2021.(in Chinese)
[20]	SAMIR H,KANDIEL M M M.Accuracy of subjective evaluation of luteal blood flow by color Doppler ultrasonography for early diagnosis of pregnancy in Egyptian buffalo[J]. Anim Reprod Sci,2019,208:106129.
[21]	JONES A K,GATELY R E,MCFADDEN K K,et al.Transabdominal ultrasound for detection of pregnancy,fetal and placental landmarks,and fetal age before day 45 of gestation in the sheep[J]. Theriogenology,2016,85(5):939-945.e1.
[22]	刘宗玲,张文倩,郭志杰,等.湖羊B超早期妊娠诊断的研究[J].中国草食动物科学,2022,42(3):63-66.LIU Z L,ZHANG W Q,GUO Z J,et al.Study on early pregnancy diagnosis of Hu sheep by B-ultrasound[J]. China Herbivore Science, 2022, 42(3):63-66.(in Chinese)
[23]	陈童,罗永明,董红,等.B超在母羊早期妊娠和繁殖疾病诊断中的应用[J].黑龙江动物繁殖,2021,29(1):43-46.CHEN T,LUO Y M,DONG H,et al.Application of B-ultrasound in diagnosis of early pregnancy and reproductive diseases of ewes[J]. Heilongjiang Journal of Animal Reproduction,2021,29(1):43-46.(in Chinese)
[24]	王骁,陈霞,张海兰,等.B超在马发情鉴定和早期妊娠诊断中的应用[J].畜牧与兽医,2018,50(4):17-21.WANG X,CHEN X,ZHANG H L,et al.Application of B-scan in estrus detection and early diagnosis of pregnancy in horses[J]. Animal Husbandry & Veterinary Medicine,2018,50(4):17-21.(in Chinese)
[25]	于克媛,李乐军,王纪宝.放射免疫法在羊妊娠诊断上的应用[J].中国畜禽种业,2019,15(9):89-90.YU K Y,LI L J,WANG J B.Application of radioimmunoassay in sheep pregnancy diagnosis[J]. The Chinese Livestock and Poultry Breeding,2019,15(9):89-90.(in Chinese)
[26]	蔡正华,方樟明,陈隆,等.用母猪血浆中硫酸雌酮浓度作早期妊娠诊断的研究[J].中国畜牧杂志,1989,25(6):12-14.CAI Z H,FANG Z M,CHEN L,et al.Pregnancy diagnosis in sows based on plasma oestrone sulphate concentration[J]. Chinese Journal of Animal Science,1989,25(6):12-14.(in Chinese)
[27]	陈海燕,傅衍.通过乳中孕酮、雌二醇含量诊断奶牛早期妊娠的研究[J].核农学报,2008,22(1):93-96.CHEN H Y,FU Y.Research of diagnosis of early pregnancy of Chinese holstein cows by measuring the contenets of progesterone and oestradiol in milk[J]. Journal of Nuclear Agricultural Sciences,2008,22(1):93-96.(in Chinese)
[28]	苏妮,孙振师,张化恒.奶牛三种早孕诊断方法的比较[J].山东畜牧兽医,2012,33(4):17-18.SU N,SUN Z S,ZHANG H H.Comparison of three diagnostic methods of early pregnancy in dairy cows[J]. Shandong Journal of Animal Science and Veterinary Medicine,2012,33(4):17-18.(in Chinese)
[29]	李永鹏.根据乳汁孕酮水平进行奶山羊早期妊娠诊断的研究[J].畜牧兽医学报,1984,15(2):18-25.LI Y P.Pragnancy diagnosis in the dairy goats from milk progesterone concentration[J]. Acta Veterinaria et Zootechnica Sinica,1984,15(2):18-25.(in Chinese)
[30]	兰兴东.牛妊娠诊断技术现状及发展方向[J].畜牧兽医科学(电子版),2021(14):140-141.LAN X D.Present situation and development direction of bovine pregnancy diagnosis technology[J]. Graziery Veterinary Sciences:Electronic Version,2021(14):140-141.(in Chinese)
[31]	刘瑞生.猪早期妊娠诊断新技术研究概况[J].养猪,2012(2):41-44.LIU R S.Overview of new techniques for early pregnancy diagnostic in pigs[J]. Swine Production,2012(2):41-44.(in Chinese)
[32]	冯建刚,魏全伟,李岩,等.酶免疫测定法检测粪便孕酮进行母猪早期妊娠诊断的研究[J].猪业科学,2014,31(5):99-101.FENG J G,WEI Q W,LI Y,et al.Study on detection of fecal progesterone by enzyme immunoassay for early pregnancy diagnosis in sows[J]. Swine Industry Science,2014,31(5):99-101.(in Chinese)
[33]	吴凌,高维明,张洪友,等.孕酮ELISA试剂盒在牛奶乳汁检测中的初步应用[J].中国畜牧杂志,2015,51(21):54-58.WU L,GAO W M,ZHANG H Y,et al.Preliminary application of progesterone ELISA kit in milk detection of dairy cows[J]. Chinese Journal of Animal Science,2015,51(21):54-58.(in Chinese)
[34]	BOSCOS C M,SAMARTZI F C,LYMBEROPOULOS A G,et al.Assessment of progesterone concentration using enzymeimmunoassay,for early pregnancy diagnosis in sheep and goats[J]. Reprod Domest Anim,2003,38(3):170-174.
[35]	赵梦圆.绵羊早期妊娠诊断关键生殖激素及差异蛋白筛选[D].晋中:山西农业大学,2019.ZHAO M Y.Screening of key reproductive hormones and differential proteins in early pregnancy diagnosis of sheep[D].Jinzhong:Shanxi Agricultural University,2019.(in Chinese)
[36]	黄丽菊,何奇松,蓝惠华,等.兽医实验室ELISA检测中常见问题的探讨[J].广东畜牧兽医科技,2022,47(5):68-70.HUANG L J,HE Q S,LAN H H,et al.Discussion on common problems in ELISA test in veterinary laboratory[J]. Guangdong Journal of Animal and Veterinary Science,2022,47(5):68-70.(in Chinese)
[37]	李学斌,杨利国,李宁.母羊血清体液免疫干扰因子的ELISA与超早期妊娠诊断[J].中国畜牧杂志,1993,29(6):9-11.LI X B,YANG L G,LI N.The enzyme linked immuno-sorbent assay of factors interfering humoral immune response and its application for super-early pregnancy diagnosis[J]. Chinese Journal of Animal Science,1993,29(6):9-11.(in Chinese)
[38]	蒋旭,李青竹,邱月红,等.羊早孕诊断试纸条的研制[J].畜禽业,2015(8):40-41.JIANG X,LI Q Z,QIU Y H,et al.Development of early pregnancy diagnostic strips in ewes[J]. Livestock and Poultry Industry,2015(8):40-41.(in Chinese)
[39]	赵纪博,库得热提·努热合买提,阿里木江·喀迪尔,等.牛早期妊娠诊断试纸的使用评价及ISG15、CD44、EFEMP1含量的测定[J].中国草食动物科学,2022,42(3):25-28,58.ZHAO J B,KDERETI·NUREHEMAITI,ALIMUJIANG·KADIER,et al.Evaluation of early pregnancy diagnostic test paper and detection of ISG15,CD44 and EFEMP1 in cattle[J]. China Herbivore Science,2022,42(3):25-28,58.(in Chinese)
[40]	许欣.湖羊早期妊娠诊断免疫胶体金层析试纸条的初步研制[D].南京:南京农业大学,2010.XU X.Development of the colloidal gold immuno-chromatographyic strips for the early pregnancy diagnosis of Hu sheep[D].Nanjing:Nanjing Agricultural University,2010.(in Chinese)
[41]	OHNUMA K,YOKOO M,ITO K,et al.Study of early pregnancy factor (EPF) in Equine (Equus caballus)[J]. Am J Reprod Immunol,2000,43(3):174-179.
[42]	赵洛冰.早孕因子在绵羊生殖器官表达与妊娠早期检测技术的研究[D].长春:吉林农业大学,2022.ZHAO L B.Study on the expression of early pregnancy factors in sheep reproductive organs and the detection technology of early pregnancy[D].Changchun:Jilin Agricultural University,2022.(in Chinese)
[43]	贺加双,马卫明,邓立新,等.玫瑰花环抑制实验检测肉牛早孕因子(EPF)活性[J].中国牛业科学,2011,37(3):11-13.HE J S,MA W M,DENG L X,et al.Detection of beef cattle pregnancy using rosette inhibition test[J]. China Cattle Science,2011,37(3):11-13.(in Chinese)
[44]	李青旺,胡建宏,王立强,等.利用硫酸铜液诊断母牛早期妊娠技术初探[J].中国农学通报,2004,20(6):1-3.LI Q W,HU J H,WANG L Q,et al.Study on the cow early pregnancy diagnose by CuSO4[J]. Chinese Agricultural Science Bulletin,2004,20(6):1-3.(in Chinese)
[45]	ZHENG M L,LI G,TAO D Y,et al.Early pregnancy diagnosis by serum acid titration in three sheep breeds[J]. Animal Husbandry and Feed Science,2011,3(1):28-29,32.
[46]	郑毛亮,李广,陶大勇,等.用血清酸滴定法对3个品种绵羊进行早期妊娠诊断的研究[J].安徽农业科学,2011, 39(8):4708-4709.ZHENG M L,LI G,TAO D Y,et al.Early pregnancy diagnosis by serum acid titration in three sheep breeds[J]. Journal of Anhui Agricultural Sciences,2011,39(8):4708-4709.(in Chinese)
[47]	陈超,张欣欣,安志高,等.奶牛早期妊娠诊断技术的研究进展[J].中国奶牛,2019(4):26-29.CHEN C,ZHANG X X,AN Z G,et al.Research progress on early pregnancy diagnosis techniques for dairy cows[J]. China Dairy Cattle,2019(4):26-29.(in Chinese)
[48]	RICCI A,CARVALHO P D,AMUNDSON M C,et al.Factors associated with pregnancy-associated glycoprotein (PAG) levels in plasma and milk of Holstein cows during early pregnancy and their effect on the accuracy of pregnancy diagnosis[J]. J Dairy Sci,2015,98(4):2502-2514.
[49]	邓亮.基于妊娠相关糖蛋白PAG1的奶牛妊娠诊断技术研究[D].长春:吉林大学,2019.DENG L.Study on pregnancy diagnosis Technology of Dairy cows based on pregnancy-associated glycoprotein PAG1[D]. Changchun:Jilin University,2019.(in Chinese)
[50]	程蕾,王定发,刘晓华,等. ISG15和OAS1 在奶牛早期妊娠阶段外周血中的表达规律[J].畜牧兽医学报,2015,46(1):77-84.CHENG L,WANG D F,LIU X H,et al.Study on ISG15 and OAS1 transcriptions in peripheral blood of dairy cows during early pregnancy[J]. Acta Veterinaria et Zootechnica Sinica,2015,46(1):77-84.(in Chinese)
[51]	LEON S A,SHAPIRO B,SKLAROFF D M,et al.Free DNA in the serum of cancer patients and the effect of therapy[J]. Cancer Res,1977,37(3):646-650.
[52]	LO Y M D,CORBETTA N,CHAMBERLAIN P F,et al.Presence of fetal DNA in maternal plasma and serum[J]. Lancet, 1997, 350(9076):485-487.
[53]	CHIM S S C,TONG Y K,CHIU R W K,et al.Detection of the placental epigenetic signature of the maspin gene in maternal plasma[J]. Proc Natl Acad Sci U S A,2005,102(41):14753-14758.
[54]	CHAN K C A,DING C M,GEROVASSILI A,et al.Hypermethylated RASSF1A in maternal plasma:a universal fetal DNA marker that improves the reliability of noninvasive prenatal diagnosis[J]. Clin Chem,2006,52(12):2211-2218.
[55]	奉淼.应用母血浆中胎儿游离DNA进行无创性产前诊断的临床分析[J].中国医药指南,2021,19(4):53-54,57.FENG M.The free fetal DNA in maternal plasma was used for clinical analysis of noninvasive prenatal diagnosis[J]. Guide of China Medicine,2021,19(4):53-54,57.(in Chinese)
[56]	王红坤,赵燕玲,陶炯,等.产前筛查结果与不良妊娠结局关系分析[J].现代妇产科进展,2021,30(3):161-166.WANG H K,ZHAO Y L,TAO J,et al.Association between adverse pregnancy outcomes and prenatal screenings[J]. Progress in Obstetrics and Gynecology,2021,30(3):161-166.(in Chinese)
[57]	席继锋,邓双义,王香祖,等.巢式PCR扩增 SRY 基因进行奶牛胚胎性别鉴定[J].江苏农业科学,2012,40(5):13-15.XI J F,DENG S Y,WANG X Z,et al.Detection of cattle fetal sex with nested PCR amplification of SRY[J]. Jiangsu Agricultural Sciences,2012,40(5):13-15.(in Chinese)
[58]	赵冰茹,黄锡霞,田可川,等.应用母体外周血中游离DNA鉴定细毛羊胎儿性别研究[J].中国畜牧杂志,2016,52(23):10-14.ZHAO B R,HUANG X X,TIAN K C,et al.Detection of fine-wool sheep fetal sex from cell-free fetal DNA in maternal peripheral blood[J]. Chinese Journal of Animal Science,2016,52(23):10-14.(in Chinese)
[59]	程文强.DNA甲基化调控 SPP 1基因参与山羊胚胎附植及妊娠早期胎盘发育的研究[D].重庆:西南大学,2021.CHENG W Q.The effect of SPP 1 gene and its DNA methylated modification on embryo implantation and placental development at early pregnancy in goats[D].Chongqing:Southwest University,2021.(in Chinese)
[60]	王瑞宏.山羊胎盘中DNA甲基化与 YY1 共同抑制 PHLDA 2基因表达[D].重庆:西南大学,2020.WANG R H.Co-suppression of PHLDA 2 expression by DNA methylation and YY1 in goat placenta[D].Chongqing:Southwest University,2020.(in Chinese)

Advance of Early Pregnancy Diagnosis in Livestock

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 60

Related Articles 3

Recommended Articles

Metrics

Comments

[1]	CAO Yantao, FAN Jiangfeng, YU Sijiu, ZHOU Yingcong, DU Peiyan, LI Yijuan, MA Jinbiao, ZHAO Shengxian. Prokaryotic Expression of Yaks EPF and Its Preparation of Polyclonal Antibodies [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 470-480.
[2]	MA Zhenyuan, WANG Shujuan, YAN Ruoqian, BAN Fuguo, ZHAO Xueli, XIE Caihua, WANG Huajun, WANG Dongfang. Establishment of Competitive Chemiluminescent Enzyme Immunoassay for Detecting Antibodies against gB Protein of Pseudorabies Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 574-583.
[3]	SU Juan;YAO Yuan;YANG Gui-hong;LI Xun;LIU Yan-peng;KOU Rui;LIU Jing;LEI Zhi-hai . Distribution of Orexin B in Porcine Hypothalamo-Pituitary-Gonadal Axis and the Regulation of Reproduction in Vitro [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(11): 1460-1466.