基于iTRAQ技术挖掘猪肾组织高原低氧适应性关键蛋白

doi:10.11843/j.issn.0366-6964.2021.05.010

Abstract

Abstract: The aim of this study was to explore the differentially expressed proteins(DEPs) of hypoxic adaptation in kidney tissues of Tibetan pigs and Yorkshire pigs under high altitude hypoxic environment. Unrelated castrated boars from Tibetan pig and Yorkshire pig were selected as experimental animals, and fed ad libitum on the plateau with altitude of 3 000 m, up to 180-day-old, 9 pigs with similar growth and body condition were randomly selected from each breed to collect kidney tissue. In this study, isobaric tags for relative and absolute quantification(iTARQ) technique was used to analyze the difference of protein expression in kidney tissues between Tibetan pigs and Yorkshire pigs, the differentially expressed proteins were analyzed by GO functional annotation and KEGG pathway. The results showed that, a total of 4 370 proteins were obtained from kidney tissues of two pig breeds, FC>1.2 or <0.833 and P<0.05 as the criterion, a total of 181 DEPs were screened out, among them, 138 proteins were up-regulated and 43 proteins were down-regulated in the Tibetan pigs. In the functional analysis, DEPs were mainly enriched in items and pathways related to hypoxic adaptation, such as HIF-1 signaling pathway, PPAR signaling pathway, complement and coagulation cascades signaling pathway, oxidoreductase activity, carbon metabolism, regulation of blood pressure, mitochondria, etc., after further comprehensive analysis, 5 important DEPs related to hypoxic adaptation were screened out(CD59, A2M, eNOS, CDKN1B and PGK1). These DEPs play an important role in maintaining hemodynamics, regulating energy metabolism and physiological environment homeostasis, the result of this study provides a theoretical basis for further study on the adaptability of pigs to hypoxic at high altitude.

Key words: iTRAQ, pig, kidney, hypoxic adaptation

CLC Number:

S828.2

LI Mengrou, WEI Mingbang, ZHANG Jian, XING Lu, XU Shijun, XIAO Qingqing, YE Yourong, DONG Shixiong, DUAN Mengqi, SHANG Peng. Mining Key Proteins of High Altitude Hypoxic Adaptability in Porcine Kidney Tissue Based on iTRAQ Technology[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1238-1246.

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Mining Key Proteins of High Altitude Hypoxic Adaptability in Porcine Kidney Tissue Based on iTRAQ Technology

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