抑制伪狂犬病病毒复制的宿主蛋白的筛选与鉴定

doi:10.11843/j.issn.0366-6964.2023.03.029

Abstract

Abstract: To screen and analyze anti-pseudorabies virus (PRV) host proteins, four differential proteins were verified by Western blot and RT-qPCR based on the results of TMT proteomics. The results were consistent with the previous results of TMT proteomics, indicating that the proteomic analysis was reliable. Eukaryotic expression vectors of four proteins were constructed, and then detected by Western blot, RT-qPCR and viral plaque assays. The results showed that all the four differential proteins could inhibit PRV replication, while the IFN-β promoter activity of MCCC1 and STRAP proteins were more significant by dual-luciferase reporter gene assay. The MCCC1 and STRAP knockdown by siRNA demonstrated that STRAP significantly promoted PRV replication. In conclusion, we found that the screened STRAP protein remarkably inhibited PRV replication, which will build the foundation for the mechanism underlying interactions between PRV-host.

Key words: TMT proteomics, PRV, DEPs, STRAP

CLC Number:

S852.659.1

HE Wenfeng, LI Chen, CHANG Hongtao, LI Longxi, CHEN Jing, YANG Guoqing, LIU Huimin. Screening and Identifying of Host Proteins that Inhibit Pseudorabies Virus Replication[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1177-1186.

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Screening and Identifying of Host Proteins that Inhibit Pseudorabies Virus Replication

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