泌乳牛不同胎次干奶期瘤胃发酵指标与甲烷产量的特征

doi:10.11843/j.issn.0366-6964.2022.12.015

Abstract

Abstract: The objectives of the present study were to investigate the effect of parity time on ruminal fermentation characteristics and methane (CH₄) production using the GreenFeed system, which examines relationships between methane (CH₄) output and animal body weight and parity time, and to use these relationships to develop prediction equations for CH₄ emission from Holstein cows at dry off in the normal physiological experimental under the housing condition. A total of 48 dry Holstein cows were selected and divided into 4 groups (12 cows in each group): first-, second-, third-, and fourth- and above-parity. The pre-trial period was 5 days and the formal trial period was 40 days. The results showed as follows: 1) The live weight and metabolic weight of third- and fourth- and above-parity groups were significantly higher than those of first- and second-parity groups (P<0.05). 2) Ammoniacal nitrogen (NH₃-N) of third-parity group was significantly higher than that of first-parity group (P<0.05), but no significant difference were found compared with the second-parity group and fourth- and above-parity groups (P>0.05); Compared with fourth- and above-parity group, microbial protein (MCP) of first-parity and second-parity groups were significantly increased (P<0.05), but there was no significant difference compared with the third-parity group (P>0.05); Compared with third-parity, valerate of first-parity group was significantly decreased (P<0.05), but there was not significantly different from the second-parity and fourth- and above-parity groups (P>0.05); The total volatile fatty acid (TVFA), pH, acetate, propionate, butyrat, isobutyrate, isovalerate, and acetate to propionate ratio were not significantly different among the treatment groups (P>0.05). 3) The average CH₄ emission of dry cows was 336 g·d^-1. The CH₄ emissions of dry cows within third-parity and fourth- and above-parity groups were significantly higher than those of first-parity group (P<0.05), and there was no significant difference compared with the group second-parity (P>0.05). 4) CH₄ emission was positively related to parity time and body weight (BW) of dry cows in the correlation coefficients of 0.47 or 0.61, respectively (P<0.01). Based on body weight to establish a prediction model CH₄(g·d^-1)=0.348×BW (kg)+64.018 (R²=0.46). It is concluded that CH₄ emission of Holstein cows at dry off can be predicted from BW. The dataset can also be used to validate a range of prediction equations for CH₄ production of cows under other physiologicalstates.

Key words: greenhouse gas, methane, GreenFeed, ruminal fermentation, cows

CLC Number:

S852.21

LIU Zhihao, JIA Peng, LAI Qi, DONG Lifeng, WU Qiujue, GAO Yanhua, TIAN Zhonghong, DIAO Qiyu. Characteristics of Rumen Fermentation and Methane Production in Different Parity Dry Cows[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4296-4305.

References 38

[1]	BLOK K,AFANADOR A,VAN DER HOORN I,et al.Assessment of sectoral greenhouse gas emission reduction potentials for 2030[J].Energies,2020,13(4):943.
[2]	IPCC.Climate change 2014-impacts,adaptation and vulnerability.Contribution of working group Ⅱ to the fifth assessment report of the intergovernmental panel on climate change[M].Cambridge:Cambridge University Press,2014.
[3]	YUSUF R O,NOOR Z Z,ABBA A H,et al.Methane emission by sectors:A comprehensive review of emission sources and mitigation methods[J].Renew Sust Energ Rev,2012,16(7):5059-5070.
[4]	娜仁花.不同日粮对奶牛瘤胃甲烷及氮排放的影响研究[D].北京:中国农业科学院,2010:3.NA R H.Effects of diet composition on methane and nitrogen emissions from lactating catttle[D].Beijing:Chinese Academy of Agricultural Sciences,2010:3.(in Chinese)
[5]	VAN GASTELEN S,DIJKSTRA J,BANNINK A.Are dietary strategies to mitigate enteric methane emission equally effective across dairy cattle,beef cattle,and sheep?[J].J Dairy Sci,2019,102(7):6109-6130.
[6]	赵一广,刁其玉,邓凯东,等.反刍动物甲烷排放的测定及调控技术研究进展[J].动物营养学报,2011,23(5):726-734.ZHAO Y G,DIAO Q Y,DENG K D,et al.Measurements and modulation of methane emission from ruminants[J].Acta Zoonutrimenta Sinica,2011,23(5):726-734.(in Chinese)
[7]	王贝.饲粮NDF/NFC对不同泌乳阶段奶牛瘤胃甲烷排放量、营养物质表观消化率及生产性能的影响[D].阿拉尔市:塔里木大学,2019:56.WANG B.Effects of dietary NDF/NFC on rumen methane emissions,nutrient apparent digestibility and production performance of dairy cows during different lactating stages[D].Alaer:Tarim University,2019:56.(in Chinese)
[8]	李斌昌.日粮精粗比对不同月龄后备奶牛甲烷排放与生长性能和营养物质消化的影响[D].兰州:甘肃农业大学,2019:20-39.LI B C.Effects of the ratios of concentrates to roughages on methane emission,growth performance and nutrient digestion of replacement heifers at different ages[D].Lanzhou:Gansu Agricultural University,2019:20-39.(in Chinese)
[9]	王小林.奶牛不同生理阶段甲烷排放规律研究及热应激对犊牛甲烷排放量的影响[D].乌鲁木齐:新疆农业大学,2012:24.WANG X L.Study on rule of methane emission of dairy cows in different physiological stages and methane emission of calves under heat stress[D].Urumqi:Xinjiang Agricultural University,2012:24.(in Chinese)
[10]	JONKER A,MOLANO G,ANTWI C,et al.Enteric methane and carbon dioxide emissions measured using respiration chambers,the sulfur hexafluoride tracer technique,and a GreenFeed head-chamber system from beef heifers fed alfalfa silage at three allowances and four feeding frequencies[J].J Anim Sci,2016,94(10):4326-4337.
[11]	张然,郑琛,闫晓刚,等.体外产气法研究牛至油对绵羊瘤胃发酵特性和甲烷产量的影响[J].动物营养学报,2018,30(8):3168-3175.ZHANG R,ZHENG C,YAN X G,et al.Effects of oregano oil on ruminal fermentation characteristics and methane production of sheep by gas production technique in vitro[J].Chinese journal of Animal Nutrition,2018,30(8):3168-3175.(in Chinese)
[12]	BRODERICK G A,KANG J H.Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J].J Dairy Sci,1980,63(1):64-75.
[13]	ZINN R A,OWENS F N.A rapid procedure for purine measurement and its use for estimating net ruminal protein synthesis[J].Can J Anim Sci,1986,66(1):157-166.
[14]	RENAND G,VINET A,DECRUYENAERE V,et al.Methane and carbon dioxide emission of beef heifers in relation with growth and feed efficiency[J].Animals,2019,9(12):1136.
[15]	HAMMOND K J,WAGHORN G C,HEGARTY R S,et al.The GreenFeed system for measurement of enteric methane emission from cattle[J].Anim Prod Sci,2016,56(3):181.
[16]	贾鹏,屠焰,李发弟,等.反刍动物甲烷排放量测定方法的研究进展[J].动物营养学报,2020,32(6):2483-2490.JIA P,TU Y,LI F D,et al.Research advance in measurement methods of methane emission from ruminants[J].Chinese journal of Animal Nutrition,2020,32(6):2483-2490.(in Chinese)
[17]	张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007.ZHANG L Y.Feed analysis and feed quality testing technology[M].3rd ed.Beijing:China Agricultural University Press,2007.(in Chinese)
[18]	KRAUSE K M,OETZEL G R.Understanding and preventing subacute ruminal acidosis in dairy herds:A review[J].Anim Feed Sci Technol,2006,126(3-4):215-236.
[19]	刘洁,刁其玉,赵一广,等.饲粮不同NFC/NDF对肉用绵羊瘤胃pH、氨态氮和挥发性脂肪酸的影响[J].动物营养学报,2012,24(6):1069-1077.LIU J,DIAO Q Y,ZHAO Y G,et al.Effects of dietary NFC/NDF ratios on rumen pH,NH3-N and VFA of meat sheep[J].Acta Zoonutrimenta Sinica,2012,24(6):1069-1077.(in Chinese)
[20]	COLEMAN G S,SANDFORD D C.The uptake and utilization of Bacteria,amino acids and nucleic acid components by the rumen ciliate Eudiplodinium maggii[J].J Appl Bacteriol,1979,47(3):409-419.
[21]	朱寿谦,李大彪,解湧芳,等.高产和低产奶牛不同泌乳阶段养分表观消化率、瘤胃发酵参数和乳品质的比较研究[J].饲料研究,2021,44(15):6-10.ZHU S Q,LI D B,XIE Y F,et al.Comparative study on apparent digestibility of nutrients,rumen fermentation parameters and milk quality of high-yielding and low-yielding dairy cows in different physiological periods[J].Feed Research,2021,44(15):6-10.(in Chinese)
[22]	VAN HOUTERT M F J.The production and metabolism of volatile fatty acids by ruminants fed roughages:A review[J].Anim Feed Sci Technol,1993,43(3-4):189-225.
[23]	李旺.瘤胃挥发性脂肪酸的作用及影响因素[J].中国畜牧杂志,2012,48(7):63-66.LI W.The effect and influence factors of rumen volatile fatty acid[J].Chinese journal of Animal Science,2012,48(7):63-66.(in Chinese)
[24]	KHAZANEHEI H,LI S,KHAFIPOUR E,et al.Effects of dry period management and parity on rumen fermentation,blood metabolites,and liver triacylglyceride in dairy cows[J].Can J Anim Sci,2015,95(3):445-453.
[25]	BOWMAN G R,BEAUCHEMIN K A,SHELFORD J A.Fibrolytic enzymes and parity effects on feeding behavior,salivation,and ruminal pH of lactating dairy cows[J].J Dairy Sci,2003,86(2):565-575.
[26]	KASUYA H,TAKAHASHI J.Methane emissions from dry cows fed grass or legume silage[J].Asian-Australas J Anim Sci,2010,23(5):563-566.
[27]	NEEL J P S,TURNER K E,GOWDA P H,et al.Effect of frame size and season on enteric methane (CH4) and carbon dioxide (CO2) emissions in Angus brood cows grazing native tallgrass prairie in central Oklahoma[J].J Anim Sci,2016,94(S5):293.
[28]	HUHTANEN P,RAMIN M,CABEZAS-GARCIA E H,et al.Effects of ruminal digesta retention time on methane emissions:A modelling approach[J].Anim Prod Sci,2016,56(3):501-506.
[29]	DIJKSTRA J,FOREES J M,FRANCE J.Quantitative aspects of ruminant digestion and metabolism[M].2nd ed.Wallingford:CABI Publishing,2005.
[30]	贾鹏,董利锋,屠焰,等.间接法测定反刍动物甲烷排放量的研究进展[J].动物营养学报,2021,33(9):4839-4847.JIA P,DONG L F,TU Y,et al.Advancements in indirect methods for measuring methane emission from ruminants[J].Chinese journal of Animal Nutrition,2021,33(9):4839-4847.(in Chinese)
[31]	BIRD-GARDINER T,ARTHUR P F,BARCHIA I M,et al.Phenotypic relationships among methane production traits assessed under ad libitum feeding of beef cattle[J].J Anim Sci,2017,95(10):4391-4398.
[32]	HERD R M,VELAZCO J I,ARTHUR P F,et al.Associations among methane emission traits measured in the feedlot and in respiration chambers in Angus cattle bred to vary in feed efficiency[J].J Anim Sci,2016,94(11):4882-4891.
[33]	DONOGHUE K A,BIRD-GARDINER T,ARTHUR P F,et al.Genetic and phenotypic variance and covariance components for methane emission and postweaning traits in Angus cattle[J].J Anim Sci,2016,94(4):1438-1445.
[34]	JONKER A,HICKEY S M,ROWE S J,et al.Genetic parameters of methane emissions determined using portable accumulation chambers in lambs and ewes grazing pasture and genetic correlations with emissions determined in respiration chambers[J].J Anim Sci,2018,96(8):3031-3042.
[35]	HUHTANEN P,CABEZAS-GARCIA E H,UTSUMI S,et al.Comparison of methods to determine methane emissions from dairy cows in farm conditions[J].J Dairy Sci,2015,98(5):3394-3409.
[36]	JIAO H P,YAN T H,WILLS D A,et al.Development of prediction models for quantification of total methane emission from enteric fermentation of young Holstein cattle at various ages[J].Agric Ecosyst Environ,2014,183:160-166.
[37]	董利锋,李斌昌,王贝,等.饲粮非纤维性碳水化合物/中性洗涤纤维对12月龄荷斯坦后备奶牛生长性能、营养物质表观消化率及瘤胃甲烷产量的影响[J].动物营养学报,2020,32(8):3688-3697.DONG L F,LI B C,WANG B,et al.Effects of dietary non-fibrous carbohydrate/neutral detergent fiber on growth performance,nutrient apparent digestibility and rumen methane production of 12-month-old Holstein dairy heifers[J].Chinese journal of Animal Nutrition,2020,32(8):3688-3697.(in Chinese)
[38]	JENTSCH W,SCHWEIGEL M,WEISSBACH F,et al.Methane production in cattle calculated by the nutrient composition of the diet[J].Arch Anim Nutr,2007,61(1):10-19.

Characteristics of Rumen Fermentation and Methane Production in Different Parity Dry Cows

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