家禽疱疹病毒CRISPR/Cas9基因编辑最新研究进展

doi:10.11843/j.issn.0366-6964.2022.10.007

Abstract

Abstract: CRISPR/Cas9-based gene editing technology, the newest generation of gene-editing technique, can target and edit nearly any kind of genes mediated by a single guide RNA (gRNA) to realize the direct genomic deletions, mutations, and/or insertions. In recent years, the application of CRISPR/Cas9 system in the large genomes of DNA viruses, especially in those of herpesviruses, has become the latest international research hotspot. Since the first report that using CRISPR/Cas9 system to edit avian herpesvirus genome such as Marek's disease virus (MDV) in 2016, this technology has been widely applied in editing the viral protein-coding genes and non-coding RNA genes, constructing gene knocked-out or recombinant vaccines, antiviral therapy as well as disease resistant breeding in recent five years. Herein, this paper reviewed the latest progresses on CRISPR/Cas9 gene editing-mediated avian herpesvirus gene function studies and vaccine developments. The prospect of CRISPR/Cas9-based gene editing is also discussed for providing important reference for future virology research.

Key words: CRISPR/Cas9, gene editing, avian herpesvirus

CLC Number:

S855.3

LUO Jun, LIU Jinling, ZHENG Luping, LUO Qin, TENG Man. Recent Advances in Engineering Avian Herpesviruses by CRISPR/Cas9-based Gene Editing Technology[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3335-3344.

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Recent Advances in Engineering Avian Herpesviruses by CRISPR/Cas9-based Gene Editing Technology

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