家禽疱疹病毒CRISPR/Cas9基因编辑最新研究进展

doi:10.11843/j.issn.0366-6964.2022.10.007

Abstract

Abstract: CRISPR/Cas9-based gene editing technology, the newest generation of gene-editing technique, can target and edit nearly any kind of genes mediated by a single guide RNA (gRNA) to realize the direct genomic deletions, mutations, and/or insertions. In recent years, the application of CRISPR/Cas9 system in the large genomes of DNA viruses, especially in those of herpesviruses, has become the latest international research hotspot. Since the first report that using CRISPR/Cas9 system to edit avian herpesvirus genome such as Marek's disease virus (MDV) in 2016, this technology has been widely applied in editing the viral protein-coding genes and non-coding RNA genes, constructing gene knocked-out or recombinant vaccines, antiviral therapy as well as disease resistant breeding in recent five years. Herein, this paper reviewed the latest progresses on CRISPR/Cas9 gene editing-mediated avian herpesvirus gene function studies and vaccine developments. The prospect of CRISPR/Cas9-based gene editing is also discussed for providing important reference for future virology research.

Key words: CRISPR/Cas9, gene editing, avian herpesvirus

CLC Number:

S855.3

LUO Jun, LIU Jinling, ZHENG Luping, LUO Qin, TENG Man. Recent Advances in Engineering Avian Herpesviruses by CRISPR/Cas9-based Gene Editing Technology[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3335-3344.

References 66

[1]	DEVEAU H, GARNEAU J E, MOINEAU S.CRISPR/Cas system and its role in phage-bacteria interactions[J].Annu Rev Microbiol, 2010, 64(1):475-493.
[2]	DELTCHEVA E, CHYLINSKI K, SHARMA C M, et al.CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III[J].Nature, 2011, 471(7340):602-607.
[3]	CONG L, RAN F A, COX D, et al.Multiplex genome engineering using CRISPR/Cas systems[J].Science, 2013, 339(6121):819-823.
[4]	MALI P, ESVELT K M, CHURCH G M.Cas9 as a versatile tool for engineering biology[J].Nat Methods, 2013, 10(10):957-963.
[5]	JIANG W Y, BIKARD D, COX D, et al.RNA-guided editing of bacterial genomes using CRISPR-Cas systems[J].Nat Biotechnol, 2013, 31(3):233-239.
[6]	CHANG N N, SUN C H, GAO L, et al.Genome editing with RNA-guided Cas9 nuclease in zebrafish embryos[J].Cell Res, 2013, 23(4):465-472.
[7]	RYAN O W, CATE J H D.Multiplex engineering of industrial yeast genomes using CRISPRm[J].Methods Enzymol, 2014, 546:473-489.
[8]	WANG H Y, YANG H, SHIVALILA C S, et al.One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering[J].Cell, 2013, 153(4):910-918.
[9]	BASSETT A, LIU J L.CRISPR/Cas9 mediated genome engineering in Drosophila[J].Methods, 2014, 69(2):128-136.
[10]	WARD J D.Rendering the intractable more tractable:tools from Caenorhabditis elegans ripe for import into parasitic nematodes[J].Genetics, 2015, 201(4):1279-1294.
[11]	KENNEDY E M, KORNEPATI A V R, CULLEN B R.Targeting hepatitis B virus cccDNA using CRISPR/Cas9[J].Antiviral Res, 2015, 123:188-192.
[12]	HU Z, YU L, ZHU D, et al.Disruption of HPV16-E7 by CRISPR/Cas system induces apoptosis and growth inhibition in HPV16 positive human cervical cancer cells[J].Biomed Res Int, 2014, 2014:612823.
[13]	BI Y W, SUN L, GAO D D, et al.High-efficiency targeted editing of large viral genomes by RNA-guided nucleases[J].PLoS Pathog, 2014, 10(5):e1004090.
[14]	WANG J B, QUAKE S R.RNA-guided endonuclease provides a therapeutic strategy to cure latent herpesviridae infection[J].Proc Natl Acad Sci U S A, 2014, 111(36):13157-13162.
[15]	GERGEN J, COULON F, CRENEGUY A, et al.Multiplex CRISPR/Cas9 system impairs HCMV replication by excising an essential viral gene[J].PLoS One, 2018, 13(2):e0192602.
[16]	TSO F Y, WEST J T, WOOD C.Reduction of Kaposi's sarcoma-associated herpesvirus latency using CRISPR-Cas9 to edit the latency-associated nuclear antigen gene[J].J Virol, 2019, 93(7):e02183-18.
[17]	XU A T, QIN C, LANG Y, et al.A simple and rapid approach to manipulate pseudorabies virus genome by CRISPR/Cas9 system[J].Biotechnol Lett, 2015, 37(6):1265-1272.
[18]	ZOU Z, HUANG K, WEI Y M, et al.Construction of a highly efficient CRISPR/Cas9-mediated duck enteritis virus-based vaccine against H5N1 avian influenza virus and duck Tembusu virus infection[J].Sci Rep, 2017, 7(1):1478.
[19]	YAO Y, BASSETT A, NAIR V.Targeted editing of avian herpesvirus vaccine vector using CRISPR/Cas9 nucleases[J].J Vaccine Technol, 2016, 1:1-7.
[20]	BAIGENT S J, PETHERBRIDGE L J, SMITH L P, et al.Herpesvirus of turkey reconstituted from bacterial artificial chromosome clones induces protection against Marek's disease[J].J Gen Virol, 2006, 87(Pt 4):769-776.
[21]	LIU Y Z, LI K, GAO Y L, et al.Recombinant Marek's disease virus as a vector-based vaccine against avian leukosis virus subgroup J in chicken[J].Viruses, 2016, 8(11):301.
[22]	BALZLI C L, BERTRAN K, LEE D H, et al.The efficacy of recombinant turkey herpesvirus vaccines targeting the H5 of highly pathogenic avian influenza virus from the 2014-2015 North American outbreak[J].Vaccine, 2018, 36(1):84-90.
[23]	LIU J X, CHEN P C, JIANG Y P, et al.A duck enteritis virus-vectored bivalent live vaccine provides fast and complete protection against H5N1 avian influenza virus infection in ducks[J].J Virol, 2011, 85(21):10989-10998.
[24]	REDDY S M, LUPIANI B, GIMENO I M, et al.Rescue of a pathogenic Marek's disease virus with overlapping cosmid DNAs:use of a pp38 mutant to validate the technology for the study of gene function[J].Proc Natl Acad Sci U S A, 2002, 99(10):7054-7059.
[25]	LUPIANI B, LEE L F, CUI X P, et al.Marek's disease virus-encoded meq gene is involved in transformation of lymphocytes but is dispensable for replication[J].Proc Natl Acad Sci U S A, 2004, 101(32):11815-11820.
[26]	SCHUMACHER D, TISCHER B K, FUCHS W, et al.Reconstitution of Marek's disease virus serotype 1 (MDV-1) from DNA cloned as a bacterial artificial chromosome and characterization of a glycoprotein B-negative MDV-1 mutant[J].J Virol, 2000, 74(23):11088-11098.
[27]	TISCHER B K, SMITH G A, OSTERRIEDER N.En passant mutagenesis:a two step markerless red recombination system[M]//BRAMAN J.In Vitro Mutagenesis Protocols.3rd ed.Humana Totowa:Springer, 2010:421-430.
[28]	PETHERBRIDGE L, HOWES K, BAIGENT S J, et al.Replication-competent bacterial artificial chromosomes of Marek's disease virus:Novel tools for generation of molecularly defined herpesvirus vaccines[J].J Virol, 2003, 77(16):8712-8718.
[29]	崔红玉.鸡马立克氏病病毒细菌人工染色体的构建[D].哈尔滨:中国农业科学院, 2007.CUI H Y.Cloning of Marek's disease virus genome as an infectious bacterial artificial chromosome[D].Harbin:Chinese Academy of Agricultural Sciences, 2007.(in Chinese).
[30]	PETHERBRIDGE L, XU H T, ZHAO Y G, et al.Cloning of Gallid herpesvirus 3 (Marek's disease virus serotype-2) genome as infectious bacterial artificial chromosomes for analysis of viral gene functions[J].J Virol Methods, 2009, 158(1-2):11-17.
[31]	PETHERBRIDGE L, BROWN A C, BAIGENT S J, et al.Oncogenicity of virulent Marek's disease virus cloned as bacterial artificial chromosomes[J].J Virol, 2004, 78(23):13376-13380.
[32]	SUN A J, LAWRENCE P, ZHAO Y G, et al.A BAC clone of MDV strain GX0101 with REV-LTR integration retained its pathogenicity[J].Chin Sci Bull, 2009, 54(15):2641-2647.
[33]	REDDY S M, SUN A J, KHAN O A, et al.Cloning of a very virulent plus, 686 strain of Marek's disease virus as a bacterial artificial chromosome[J].Avian Dis, 2013, 57(2 Suppl):469-473.
[34]	JAROSINSKI K W, MARGULIS N G, KAMIL J P, et al.Horizontal transmission of Marek's disease virus requires US2, the UL13 protein kinase, and gC[J].J Virol, 2007, 81(19):10575-10587.
[35]	SPATZ S J, SMITH L P, BAIGENT S J, et al.Genotypic characterization of two bacterial artificial chromosome clones derived from a single DNA source of the very virulent gallid herpesvirus-2 strain C12/130[J].J Gen Virol, 2011, 92(Pt 7):1500-1507.
[36]	AFONSO C L, TULMAN E R, LU Z, et al.The genome of Turkey herpesvirus[J].J Virol, 2001, 75(2):971-978.
[37]	BUBLOT M, SHARMA J.Vaccination against Marek's disease[M]//DAVISON F, NAIR V.Marek's Disease.Amsterdam:Elsevier, 2004:168-185.
[38]	CRONENBERG A M, VAN GEFFEN C E, DORRESTEIN J, et al.Vaccination of broilers with HVT expressing an Eimeria acervulina antigen improves performance after challenge with Eimeria[J].Acta Virol, 1999, 43(2-3):192-197.
[39]	LI Y Q, REDDY K, REID S M, et al.Recombinant herpesvirus of turkeys as a vector-based vaccine against highly pathogenic H7N1 avian influenza and Marek's disease[J].Vaccine, 2011, 29(46):8257-8266.
[40]	REDDY S K, SHARMA J M, AHMAD J, et al.Protective efficacy of a recombinant herpesvirus of turkeys as an in ovo vaccine against Newcastle and Marek's diseases in specific-pathogen-free chickens[J].Vaccine, 1996, 14(6):469-477.
[41]	TSUKAMOTO K, SAITO S, SAEKI S, et al.Complete, long-lasting protection against lethal infectious bursal disease virus challenge by a single vaccination with an avian herpesvirus vector expressing VP2 antigens[J].J Virol, 2002, 76(11):5637-5645.
[42]	HECKERT R A, RIVA J, COOK S, et al.Onset of protective immunity in chicks after vaccination with a recombinant herpesvirus of turkeys vaccine expressing Newcastle disease virus fusion and hemagglutinin-neuraminidase antigens[J].Avian Dis, 1996, 40(4):770-777.
[43]	ZHANG Y Y, TANG N, SADIGH Y, et al.Application of CRISPR/Cas9 gene editing system on MDV-1 genome for the study of gene function[J].Viruses, 2018, 10(6):279.
[44]	张峰, 于正浩, 兰兴鸽, 等.利用CRISPR/Cas9技术缺失MDV分离株meq基因的研究[J].中国家禽, 2019, 41(9):26-32.ZHANG F, YU Z H, LAN X G, et al.Study on deletion of meq gene of MDV isolate by CRISPR/Cas9 system[J].China Poultry, 2019, 41(9):26-32.(in Chinese)
[45]	滕蔓, 郑鹿平, 刘金玲, 等.利用CRISPR/Cas9基因编辑技术构建马立克病病毒超强毒株原癌基因meq缺失株及其鉴定[J].病毒学报, 2020, 36(4):675-684.TENG M, ZHENG L P, LIU J L, et al.Editing of oncogenic meq of very-virulent Marek's disease virus by the CRISPR/Cas9 system[J].Chinese Journal of Virology, 2020, 36(4):675-684.(in Chinese)
[46]	杨森, 滕蔓, 刘金玲, 等.鸡马立克病疫苗株CVI988/Rispens meq基因编辑及缺失毒株的构建与鉴定[J].畜牧兽医学报, 2020, 51(8):1970-1976.YANG S, TENG M, LIU J L, et al.Construction of meq deleted strain by gene editing of Marek's disease vaccine strain CVI988/Rispens via the CRISPR/Cas9 system and identification[J].Acta Veterinaria et Zootechnica Sinica, 2020, 51(8):1970-1976.(in Chinese)
[47]	LUO J, TENG M, ZAI X S, et al.Efficient mutagenesis of Marek's disease virus-encoded microRNAs using a CRISPR/Cas9-based gene editing system[J].Viruses, 2020, 12(4):466.
[48]	YU Z H, TENG M, SUN A J, et al.Virus-encoded miR-155 ortholog is an important potential regulator but not essential for the development of lymphomas induced by very virulent Marek's disease virus[J].Virology, 2014, 448:55-64.
[49]	TENG M, YU Z H, SUN A J, et al.The significance of the individual meq-clustered miRNAs of Marek's disease virus in oncogenesis[J].J Gen Virol, 2015, 96(Pt 3):637-649.
[50]	TENG M, YU Z H, ZHAO P, et al.Putative roles as oncogene or tumour suppressor of the Mid-clustered microRNAs in Gallid alphaherpesvirus 2 (GaHV2) induced Marek's disease lymphomagenesis[J].J Gen Virol, 2017, 98(5):1097-1112.
[51]	楚钰淑, 滕蔓, 周子誉, 等.LAT基因簇miRNA的CRISPR/Cas9基因编辑对马立克病病毒体外复制的影响分析[J].病毒学报, 2021, 37(6):1428-1439.CHU Y S, TENG M, ZHOU Z Y, et al.CRISPR/Cas9 system-based gene editing of the LAT-clustered microRNAs and its influence on in vitro replication of Marek's disease virus[J].Chinese Journal of Virology, 2021, 37(6):1428-1439.(in Chinese)
[52]	ZHAO Y G, PETHERBRIDGE L, SMITH L P, et al.Self-excision of the BAC sequences from the recombinant Marek's disease virus genome increases replication and pathogenicity[J].Virol J, 2008, 5:19.
[53]	ZHANG Y Y, LUO J, TANG N, et al.Targeted editing of the pp38 gene in Marek's disease virus-transformed cell lines using CRISPR/Cas9 system[J].Viruses, 2019, 11(5):391.
[54]	ZHANG Y Y, TANG N, LUO J, et al.Marek's disease virus-encoded microRNA 155 ortholog critical for the induction of lymphomas is not essential for the proliferation of transformed cell lines[J].J Virol, 2019, 93(17):e00713-19.
[55]	TANG N, ZHANG Y Y, PEDRERA M, et al.A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system[J].Vaccine, 2018, 36(5):716-722.
[56]	TANG N, ZHANG Y Y, SADIGH Y, et al.Generation of a triple insert live avian herpesvirus vectored vaccine using CRISPR/Cas9-based gene editing[J].Vaccines (Basel), 2020, 8(1):97.
[57]	CHANG P X, AMEEN F, SEALY J E, et al.Application of HDR-CRISPR/Cas9 and erythrocyte binding for rapid generation of recombinant turkey herpesvirus-vectored avian influenza virus vaccines[J].Vaccines (Basel), 2019, 7(4):192.
[58]	LIU L T, WANG T, WANG M Y, et al.Recombinant turkey herpesvirus expressing H9 hemagglutinin providing protection against H9N2 avian influenza[J].Virology, 2019, 529:7-15.
[59]	HAGAG I T, WIGHT D J, BARTSCH D, et al.Abrogation of Marek's disease virus replication using CRISPR/Cas9[J].Sci Rep, 2020, 10(1):10919.
[60]	CHALLAGULLA A, JENKINS K A, O'NEIL T E, et al.In vivo inhibition of Marek's disease virus in transgenic chickens expressing Cas9 and gRNA against ICP4[J].Microorganisms, 2021, 9(1):164.
[61]	LIU Y Z, XU Z K, ZHANG Y, et al.Marek's disease virus as a CRISPR/Cas9 delivery system to defend against avian leukosis virus infection in chickens[J].Vet Microbiol, 2020, 242:108589.
[62]	LI K, LIU Y Z, XU Z K, et al.Prevention of avian retrovirus infection in chickens using CRISPR-Cas9 delivered by Marek's disease virus[J].Mol Ther Nucleic Acids, 2020, 21:343-353.
[63]	CHANG P X, YAO Y X, TANG N, et al.The application of NHEJ-CRISPR/Cas9 and Cre-Lox system in the generation of bivalent duck enteritis virus vaccine against avian influenza virus[J].Viruses, 2018, 10(2):81.
[64]	ATASOY M O, ROHAIM M A, MUNIR M.Simultaneous deletion of virulence factors and insertion of antigens into the infectious laryngotracheitis virus using NHEJ-CRISPR/Cas9 and Cre-Lox system for construction of a stable vaccine vector[J].Vaccines (Basel), 2019, 7(4):207.
[65]	滕蔓, 刘金玲, 郑鹿平, 等.CRISPR/Cas9基因编辑技术在病毒学研究中的应用及进展[J].病毒学报, 2020, 36(5):946-954.TENG M, LIU J L, ZHENG L P, et al.Research progress in application of the CRISPR/Cas9 gene editing system in virology[J].Chinese Journal of Virology, 2020, 36(5):946-954.(in Chinese)
[66]	TENG M, YAO Y X, NAIR V, et al.Latest advances of virology research using CRISPR/Cas9-based gene-editing technology and its application to vaccine development[J].Viruses, 2021, 13(5):779.

Recent Advances in Engineering Avian Herpesviruses by CRISPR/Cas9-based Gene Editing Technology

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