引导编辑系统的研究与应用进展

doi:10.11843/j.issn.0366-6964.2022.11.001

Abstract

Abstract: Prime editing system is a novel CRISPR/Cas9-derived genome-editing technology, which enables all 12 base-to-base conversions, precise small insertions and deletions without requiring double-strand breaks or exogenous donor DNA templates. This paper gives a detailed review of the basic research in recent years from the development process of gene editing, the composition and characteristics of the prime editing system, the optimization of the prime editing system, the current application of the prime editing system in animals and plants, the design of the prime editing system and off-target effects. The information presented may facilitate interested researchers to understand the prime editing system and further promoting the application of prime editing system in basic research and breeding of animals and plants.

Key words: gene editing technology, prime editing system, precise editing, application research

CLC Number:

S813

ZOU Huiying, LI Junliang, ZHU Huabin. Progress on Research and Application of Prime Editing System[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3721-3730.

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Progress on Research and Application of Prime Editing System

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