CRISPR/Cas9系统在寄生原虫基因编辑中的应用

doi:10.11843/j.issn.0366-6964.2023.01.007

Abstract

Abstract: Protozoa have complex life cycle and most of them can not complete the life cycle just in vitro. As a result, most of them lack an effective gene editing system. The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (endonuclease) system facilitates the precise, efficient, and rapid genetic editing through homologous recombination, and is widely used in genetic modification of eukaryotic organisms. The CRISPR/Cas9 system opens up a new avenue for targeted investigation of gene functions in protozoans. This article introduces the principle of the CRISPR/Cas9 system and its major applications in gene tagging and depletion and genome-wide screening of functional gene families in parasitic protozoan.

Key words: CRISPR/Cas9, genetic editing, parasite, protozoa

CLC Number:

S852.73

DENG Min'er, LI Na, GUO Yaqiong, FENG Yaoyu, XIAO Lihua. Application of CRISPR/Cas9 System on Gene Editing of Parasitic Protozoa[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 69-79.

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Application of CRISPR/Cas9 System on Gene Editing of Parasitic Protozoa

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