新疆牛、羊和骆驼源产志贺毒素大肠埃希菌的系统进化分群、血清群与毒力基因及耐药性分析

doi:10.11843/j.issn.0366-6964.2021.012.032

Abstract

Abstract: This study was conducted to examine the distribution of phylogenetic clustering, serotype, virulence genes, drug resistance and genetic diversity of Shiga toxin-producing Escherichia coli (STEC) from cattle, sheep and camel in Xinjiang. Phylogenetic clustering, serotype and virulence genes stx₁, stx₂ (including subtypes), eaeA and hlyA of STEC isolates were tested by PCR method from cattle, sheep and camel, drug sensitivity of isolates was determined by using a Kirby-Bauer disk diffusion method and genotypical analysis was performed by enterobacterial repetitive intergenic consensus (ERIC) polymerase chain reaction (PCR). The results showed that 94 of non-O157 STEC isolates which were mainly B1 group and contained 9 serum groups, including O146 (n=14), O22 (n=7), O3 (n=4), O168 (n=4), O8 (n=3), O167 (n=2), O88 (n=1), O112ab (n=1) and O147 (n=1). The virulence gene test of showed that 46.8% (44/94) carried stx₁ only, 6.4% (6/94) for stx₂ only, and 46.8% (44/94) carried both stx₁ and stx₂. STEC isolated from sheep and camel mainly carried stx₁+hlyA (68.0% and 25.0%, respectively), while STEC isolated from cattle mainly carried stx₁+stx₂+hlyA (57.9%). stx_1awas mainly distributed in bovine STEC and stx_1cwas mainly distributed in STEC of sheep. Fourteen strains (14.9%) were drug-resistant, and the drug resistance rates of STEC isolates to ceftazidime, tetracycline, cefotaxime, ampicillin and amtronam ranged from 3.2% to 5.3%, to cotrimoxazole, cefepime, piperacillin-tazobactam, ampicillin-sulbactam, amoxicillin-clavulanate and polymyxin B ranged from 1.1% to 2.1%. ERIC-PCR results showed that STEC from cattle, sheep and camel were closely related. Cattle, sheep and camels carry a variety of known serotypes of STEC and store abundant virulence genes, which may potentially infect human beings. Therefore, it is necessary to prevent and control the contamination of meat during slaughtering and processing.

Key words: Shiga toxin-producing Escherichia coli, ruminants, virulence gene, drug resistance, ERIC-PCR

CLC Number:

TONG Panpan, ZHANG Mengmeng, CHEN Wenxia, LIU Luyao, ZHANG Ling, TANG Xuelin, SU Zhanqiang, XIE Jinxin. Phylogenetic Clustering, Serotype, Virulence Genes and Drug Resistance Analysis of Shiga Toxin-producing Escherichia coli from Cattle, Sheep and Camel in Xinjiang[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3660-3668.

References 31

[1]	KARMALI M A, GANNON V, SARGEANT J M. Verocytotoxin-producing Escherichia coli (VTEC)[J]. Vet Microbiol, 2010, 140(3-4):360-370.
[2]	EFSA, ECDC. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2009[J]. EFSA J, 2011, 9(3):2090.
[3]	SCHEUTZ F, TEEL L D, BEUTIN L, et al. Multicenter evaluation of a sequence-based protocol for subtyping Shiga toxins and standardizing Stx nomenclature[J]. J Clin Microbiol, 2012, 50(9):2951-2963.
[4]	TSENG M, FRATAMICO P M, BAGI L, et al. Diverse virulence gene content of Shiga toxin-producing Escherichia coli from finishing swine[J]. Appl Environ Microbiol, 2014, 80(20):6395-6402.
[5]	FRIEDRICH A W, BIELASZEWSKA M, ZHANG W L, et al. Escherichia coli harboring Shiga toxin 2 gene variants:frequency and association with clinical symptoms[J]. J Infect Dis, 2002, 185(1):74-84.
[6]	MCWILLIAMS B D, TORRES A G. Enterohemorrhagic Escherichia coli adhesins[J]. Microbiol Spectr, 2014, 2(3):1-19.
[7]	TORO M, RIVERA D, JIMÉNEZ M F, et al. Isolation and characterization of non-O157 Shiga toxin-producing Escherichia coli (STEC) isolated from retail ground beef in Santiago, Chile[J]. Food Microbiol, 2018, 75:55-60.
[8]	JAJARMI M, FOOLADI A A I, BADOUEI M A, et al. Virulence genes, Shiga toxin subtypes, major O-serogroups, and phylogenetic background of Shiga toxin-producing Escherichia coli strains isolated from cattle in Iran[J]. Microb Pathog, 2017, 109:274-279.
[9]	白向宁, 王红, 赵爱兰, 等. 食源性产志贺毒素大肠杆菌的分离及菌株特征分析[J]. 中国食品卫生杂志, 2014, 26(4):312-317.BAI X N, WANG H, ZHAO A L, et al. Isolation and characterization of Shiga toxin-producing Escherichia coli isolates in foods[J]. Chinese Journal of Food Hygiene, 2014, 26(4):312-317. (in Chinese)
[10]	PARK H J, YOON J W, HEO E J, et al. Antibiotic resistance and virulence potentials of Shiga toxin-producing Escherichia coli isolates from raw meats of slaughterhouses and retail markets in Korea[J]. J Microbiol Biotechnol, 2015, 25(9):1460-1466.
[11]	EFSA, ECDC. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2017[J]. EFSA J, 2018, 16(12):e05500.
[12]	SALEHI T Z, TONELLI A, MAZZA A, et al. Genetic characterization of Escherichia coli O157:H7 strains isolated from the one-humped camel (Camelus dromedarius) by using microarray DNA technology[J]. Mol Biotechnol, 2012, 51(3):283-288.
[13]	SHARP A, SMOUT E, BYRNE L, et al. An outbreak of Shiga toxin-producing Escherichia coli O157:H7 linked to a mud-based obstacle course, England, August 2018[J]. Zoonoses Public Health, 2020, 67(5):467-473.
[14]	薛涛, 顾丛丛, 高崧, 等. 江苏某奶牛场健康奶牛体内产志贺毒素大肠杆菌的分离鉴定及其对小鼠致病性的研究[J]. 畜牧兽医学报, 2011, 42(6):830-837.XUE T, GU C C, GAO S, et al. Identification of Shiga toxin-producing Escherichia coli from healthy cattle in one farm of Jiangsu province and their pathogenicity on mice[J]. Acta Veterinaria et Zootechnica Sinica, 2011, 42(6):830-837. (in Chinese)
[15]	刘飞, 宋定州, 汤承. 川西北牦牛产志贺毒素大肠杆菌的流行病学调查[J]. 西南民族大学学报:自然科学版, 2014, 40(5):652-658.LIU F, SONG D Z, TANG C. Epidemiological survey of Shiga toxin-producing Escherichia coli from yak (Bos grunniens) on the northwestern Sichuan grassland[J]. Journal of Southwest University for Nationalities:Natrual Science Edition, 2014, 40(5):652-658. (in Chinese)
[16]	顾丛丛, 薛涛, 徐婷婷, 等. 江苏某地健康绵羊群产志贺毒素大肠杆菌体内分离株的分子流行病学及致病力[J]. 微生物学报2011, 51(5):676-683.GU C C, XUE T, XU T T, et al. Epidemiological investigation of Shiga toxin-producing Escherichia coli isolates originated from healthy sheep in one farm of Jiangsu province and their pathogenicity[J]. Acta Microbiologica Sinica, 2011, 51(5):676-683. (in Chinese)
[17]	CLERMONT O, CHRISTENSON J K, DENAMUR E, et al. The Clermont Escherichia coli phylo-typing method revisited:improvement of specificity and detection of new phylo-groups[J]. Environ Microbiol Rep, 2013, 5(1):58-65.
[18]	IGUCHI A, IYODA S, SETO K, et al. Escherichia coli O-genotyping PCR:a comprehensive and practical platform for molecular O serogrouping[J]. J Clin Microbiol, 2015, 53(8):2427-2432.
[19]	PATON A W, PATON J C. Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157[J]. J Clin Microbiol, 1998, 36(2):598-602.
[20]	Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing;Twenty-nine informational supplement[S]. PA:CLSI, 2019.
[21]	MAGIORAKOS A P, SRINIVASAN A, CAREY R B, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria:an international expert proposal for interim standard definitions for acquired resistance[J]. Clin Microbiol Infect, 2012, 18(3):268-281.
[22]	VERSALOVIC J, KOEUTH T, LUPSKI R. Distribution of repetitive DNA sequences in eubacteria and application to finerpriting of bacterial enomes[J]. Nucleic Acids Res, 1991, 19(24):6823-6831.
[23]	ALIZADE H, NEKOUBIN M, GHANBARPOUR R. Phylogenetic of Shiga toxin-producing Escherichia coli and atypical enteropathogenic Escherichia coli strains isolated from human and cattle in Kerman, Iran[J]. Methods Mol Biol, 2014, 766(766):239-248.
[24]	OLIVEIRA M G, BRITO J R F, GOMES T A T, et al. Diversity of virulence profiles of Shiga toxin-producing Escherichia coli serotypes in food-producing animals in Brazil[J]. Int J Food Microbiol, 2008, 127(1-2):139-146.
[25]	DAGA A P, KOGA V L, SONCINI J G M, et al. Escherichia coli bloodstream infections in patients at a university hospital:virulence factors and clinical characteristics[J]. Front Cell Infect Microbiol, 2019, 9:191.
[26]	BADOUEI M A, JAJARMI M, MIRSALEHIAN A. Virulence profiling and genetic relatedness of Shiga toxin-producing Escherichia coli isolated from humans and ruminants[J]. Comp Immunol Microbiol Infect Dis, 2015, 38:15-20.
[27]	BESSALAH S, FAIRBROTHER J M, SALHI I, et al. Characterization and antimicrobial susceptibility of Escherichia coli isolated from healthy farm animals in Tunisia[J]. Anim Biotechnol, 2020(4):1-10.
[28]	RAMACHANDRAN V, HORNITZKY M A, BETTELHEIM K A, et al. The common ovine Shiga toxin 2-containing Escherichia coli serotypes and human isolates of the same serotypes possess a Stx2 d toxin type[J]. J Clin Microbiol, 2001, 39(5):1932-1937.
[29]	佟盼盼, 马凯琪, 刘争辉, 等. 新疆牛源产志贺毒素大肠杆菌耐药性及其ESBLs基因鉴定[J]. 畜牧兽医学报, 2019, 50(4):887-892.TONG P P, MA K Q, LIU Z H, et al. Characterization of antimicrobial resistance and extended-spectrum β-lactamase genes in bovine origin Shiga toxin-producing Escherichia coli in Xinjiang[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(4):887-892. (in Chinese)
[30]	DONG P C, XIAO T T, NYCHAS G J E, et al. Occurrence and characterization of Shiga toxin-producing Escherichia coli (STEC) isolated from Chinese beef processing plants[J]. Meat Sci, 2020, 168:108188.
[31]	郑晓风, 张妍, 刘英玉, 等. 新疆部分地区牛羊源产志贺毒素大肠埃希菌菌株检测与分析[J]. 畜牧兽医学报, 2020, 51(10):2518-2527.ZHENG X F, ZHANG Y, LIU Y Y, et al. Detection and analysis of Shiga toxin-producing Escherichia coli isolates from cattle and sheep sources in some regions of Xinjiang, China[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10):2518-2527. (in Chinese)

Phylogenetic Clustering, Serotype, Virulence Genes and Drug Resistance Analysis of Shiga Toxin-producing Escherichia coli from Cattle, Sheep and Camel in Xinjiang

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 31

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHENG Rui, LIU Zishi, ZHANG Kangyou, YAN Yong, WEI Ling, ZEREN Wengmu, DINGZE Demi, HUANG Jianjun, WANG Li, WEI Yong. Isolation, Identification and Biological Characterization of Colletotrichum jasminigenum in Stems of Peanuts [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2206-2213.
[2]	ZHANG Yanmin, ZHAO Dongxu, WANG Wenlong. Mechanism of Resistance to Ivermectin in the Haemonchus contortus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1511-1520.
[3]	SONG Yan, YUAN Yongfeng, QIAN Hongyu, LI Xincan, LUO Hongyan, WANG Zhiying, ZHOU Zuoyong. Identification and Partial Biological Characteristics Analysis of Corynebacterium pseudotuberculosis Isolated from Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 680-687.
[4]	LIU Xinhuan, YUN Jialei, MAO Li, LI Jizong, HAO Fei, HE Miaofeng, YANG Leilei, ZHANG Wenwen, CHENG Zilong, SUN Min, LIU Maojun, WANG Shaohui, BAI Juan, LI Wenliang. Isolation, Identification, Virulence Genes and Drug Resistance Analysis of Escherichia coli Isolated from Diarrheal Goat and Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3445-3454.
[5]	YU Shixiong, WEI Lingyun, XU Tiantian, JIAO Jinzhen, JIANG Linshu, HE Zhixiong. Research Progress of Intestinal Microbial Colonization Pattern in Young Ruminants and Its Nutritional Regulation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2701-2707.
[6]	WANG Yu, GAO Yueyi, GAO Jinyuan, LIU Weijie, XU Huilin, XUE Qinghong. Establishment of Reverse Genetics System of Peste des petits Ruminants Virus Clone9 Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2956-2963.
[7]	ZHAO Feifei, LI Jie, HAN Ning, XIE Shiting, ZENG Zhenling. Antibacterial Drug Resistance Analysis of Klebsiella pneumoniae Isolated from Slaughterhouse [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 3044-3053.
[8]	ZHAO Wei, Mahmoud M. Abdelsattar, CHAI Jianmin, WANG Xin, DIAO Qiyu, ZHANG Naifeng. Research Progress of Rumen Microbiota Transplantation and Its Application [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1792-1803.
[9]	FANG Yuan, HOU Qiaodi, XIANG Chaohui, ZHAO Hongyi, QI Xuefeng. Regulatory Effects of IFITM3 on Proliferation of Peste des Petits Ruminants Virus (PPRV) in Goat Endometrial Epithelial Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2200-2207.
[10]	JIANG Zenghai, TENG Lin, HE Anwen, LIU Yanyan, YUE Min, HE Qigai. Genomic Analysis of Salmonella Typhimurium Isolates and Salmonella Serotype 4, [5], 12: i:- Isolates from Pig-borne Food Chain [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1199-1209.
[11]	Lü Ruoyi, SI Xiaohui, SUN Zhigang, SHI Xiaomin, LIU Xiaoye. Drug Resistance Situation of Streptococcus suis and Prevention Measures of Infections [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4920-4933.
[12]	WANG Jianing, ZHANG Ziqiang, KONG Dejing, FENG Caicai, ZHANG Feike, LIU Yumei. Isolation and Identification of Klebsiella pneumoniae in Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5198-5206.
[13]	YANG Menglin, ZHENG Shiqi, PENG Kai, WANG Wei, HUANG Yanhua, PENG Jie. Isolation and Identification of Pigeon-derived Salmonella Typhimurium and Pathogenic Analysis [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4880-4888.
[14]	WANG Yiqun, LIU Zupei, LI Yating, ZHANG Haisen, LI Dan, JIN Yaping, CHEN Huatao. The Dairy Cow NR1D1 Gene’s Eukaryotic Expression Vector Construction, Expression Profile and Its Ovarian Localization [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 133-145.
[15]	ZHANG Kaichuan, WANG Jinyu, LI Shoujun, JIA Kun. Isolation, Identification and Biological Characteristics of Klebsiella pneumoniae from Sheep in Guangdong Province [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 328-337.