短管兔耳草多糖的结构表征及其免疫调节活性研究

doi:10.11843/j.issn.0366-6964.2021.011.028

Abstract

Abstract: The purpose of this study was to isolate the polysaccharide from Lagotis brevituba Maxim (LMP) and to investigate the structural features and its immunomodulatory activity in vitro. LMP was obtained by water extraction and ethanol precipitation, and purified sequentially by DEAE-52 column chromatography. Subsequently, high performance gel permeation chromatography (HPGPC), fourier transform infrared spectroscopy (FT-IR), ultraviolet spectroscopy (UV) were employed to characterize the structural properties of LMP. The immunomodulatory activities of LMP were studied by MTT, FCM and ELISA, and using murine bone marrow derived dendritic cells (BMDCs) as the target cells. The results showed that LMP was obtained from Lagotis brevituba Maxim and contained no nucleic acid and protein. The yield and total carbohydrate content of LMP were 18.5%±1.7% and 89.7%±1.9%, respectively, and the average molecular weight of LMP was 3.18 ku. In addition, the average particle size and Zeta potentials of LMP were 1 483.89 nm and-14.81 mV, respectively. SEM and AFM images showed that LMP was a sheet-like appearance with smooth surface and uniformly dispersed in height of 5.4 nm. At 3.13-50 μg·mL^-1, LMP promoted the cell proliferation of BMDCs, and significantly increased the expression of surface markers (CD80, CD86, MHC-Ⅰ and MHC-Ⅱ) (P<0.05) and the secretion content of cytokines (TNF-α and IL-12) (P<0.05). Moreover, LMP decreased the phagocytosis of BMDCs (P<0.05). LMP showed better immune activity and it could induce the maturation of the phenotype and functions of BMDCs, which would be potentially developed as an effective immunomodulatory agent.

Key words: Lagotis brevituba Maxim, polysaccharides, characterization, dendritic cells, immunomodulation

CLC Number:

S853.74

YU Ruihong, MENG Zhen, SUN Mengke, CHEN Shixiong, ZHANG Junwen, HUANG Yifan, QIN Tao, REN Zhe. Structural Characterization and Immunomodulatory Activities of Polysaccharide Extracted from Lagotis brevituba Maxim[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3270-3281.

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Structural Characterization and Immunomodulatory Activities of Polysaccharide Extracted from Lagotis brevituba Maxim

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