中药多糖作为免疫增强剂及其对肠道黏膜影响的研究进展

doi:10.11843/j.issn.0366-6964.2022.07.004

Abstract

Abstract: Mucosa is the frontline of body immune defense. Protective mucosal immune response can be effectively induced by oral administration, nose drop, ocular routes and other mucosal routes. However, most mucosal immune vaccines have not been developed, and there is a lack of safe and effective immunopotentiator. Chinese herbal polysaccharides have been proved as biologically-active macromolecules to promote mucosal immune response. This paper reviews different mucosal immunopotentiator of Chinese herbal polysaccharides and its effect on intestinal mucosa in recent years, in order to provide new ideas for the development and utilization of Chinese herbal polysaccharides as mucosal immune immunopotentiator.

Key words: Chinese herbal polysaccharides, intestinal mucosal immunity, immunopotentiator

CLC Number:

S853.74

BO Ruonan, WANG Jing, LIU Xiaopan, LIU Mingjiang, LI Jingui. Research Progress of Chinese Herbal Polysaccharides as Immunopotentiator and Its Effect on Intestinal Mucosa[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2066-2073.

References 75

[1]	RICHARDS A F, TORRES-VELEZ F J, MANTIS N J. Salmonella uptake into gut-associated lymphoid tissues:implications for targeted mucosal vaccine design and delivery[J]. Methods Mol Biol, 2022, 2410:305-324.
[2]	PEREZ-LOPEZ A, BEHNSEN J, NUCCIO S P, et al. Mucosal immunity to pathogenic intestinal bacteria[J]. Nat Rev Immunol, 2016, 16(3):135-148.
[3]	CARVALHO M F, GILL D. Rotavirus vaccine efficacy:current status and areas for improvement[J]. Hum Vaccin Immunother, 2019, 15(6):1237-1250.
[4]	LI M, WANG Y, SUN Y, et al. Mucosal vaccines:strategies and challenges[J]. Immunol Lett, 2020, 217:116-125.
[5]	CRISCUOLO E, CAPUTO V, DIOTTI R A, et al. Alternative methods of vaccine delivery:an overview of edible and intradermal vaccines[J]. J Immunol Res, 2019, 2019:8303648.
[6]	SHAKYA A K, CHOWDHURY M Y E, TAO W Q, et al. Mucosal vaccine delivery:current state and a pediatric perspective[J]. J Control Release, 2016, 240:394-413.
[7]	FUJIMOTO K, UEMATSU S. Development of prime-boost-type next-generation mucosal vaccines[J]. Int Immunol, 2020, 32(9):597-603.
[8]	LONGET S, LUNDAHL M L E, LAVELLE E C. Targeted strategies for mucosal vaccination[J]. Bioconjugate Chem, 2018, 29(3):613-623.
[9]	MIQUEL-CLOPÉS A, BENTLEY E G, STEWART J P, et al. Mucosal vaccines and technology[J]. Clin Exp Immunol, 2019, 196(2):205-214.
[10]	AOSHI T. Modes of action for mucosal vaccine adjuvants[J]. Viral Immunol, 2017, 30(6):463-470.
[11]	SUN B N, YU S, ZHAO D Y, et al. Polysaccharides as vaccine adjuvants[J]. Vaccine, 2018, 36(35):5226-5234.
[12]	WAN X H, YIN Y M, ZHOU C Z, et al. Polysaccharides derived from Chinese medicinal herbs:a promising choice of vaccine adjuvants[J]. Carbohydr Polym, 2022, 276:118739.
[13]	徐书雯.胞内pH敏感型黄芪多糖PLGA纳米粒作为抗原佐剂的研究[D].南京:南京农业大学, 2019.XU S W. Study on the intracellular pH-responsive astragalus polysaccharide-encapsulated PLGA nanoparticles as adjuvant for antigens[D]. Nanjing:Nanjing Agricultural University, 2019.(in Chinese)
[14]	刘振广.灵芝多糖脂质体和脂质立方液晶纳米粒免疫增强作用的研究[D].南京:南京农业大学, 2018.LIU Z G. Study on immunological enhancement activity of ganoderma lucidum polysaccharide liposome and cubosome[D]. Nanjing:Nanjing Agricultural University, 2018.(in Chinese)
[15]	罗莉.山药多糖PLGA纳米粒的制备及其免疫增强作用的研究[D].南京:南京农业大学, 2017.LUO L. Study on preparation and immunological enhancement activity of Chinese Yam polysaccharide PLGA nanoparticles[D]. Nanjing:Nanjing Agricultural University, 2017.(in Chinese)
[16]	CHEN X Y, HAN W W, WANG G X, et al. Application prospect of polysaccharides in the development of anti-novel coronavirus drugs and vaccines[J]. Int J Biol Macromol, 2020, 164:331-343.
[17]	KWAK M, YU K, LEE P C W, et al. Rehmannia glutinosa polysaccharide functions as a mucosal adjuvant to induce dendritic cell activation in mediastinal lymph node[J]. Int J Biol Macromol, 2018, 120:1618-1623.
[18]	LIAO L Y, LI J, LI J, et al. Effects of Astragalus polysaccharides on intestinal morphology and intestinal immune cells of Muscovy ducklings infected with Muscovy duck reovirus[J]. Poult Sci, 2021, 100(1):64-72.
[19]	任广明.香菇多糖的硒化及其对慢性胰腺炎小鼠肠道菌群的影响[D].哈尔滨:东北农业大学, 2016.REN G M. Research on selenylation of lentinan and it's effects on gut microbiota in chronic pancreatitis mice[D]. Harbin:Northeast Agricultural University, 2016.(in Chinese)
[20]	赵明明.黑灵芝多糖对小鼠肠道黏膜免疫及黏膜损伤的影响[D].南昌:南昌大学, 2018.ZHAO M M. Effect of polysaccharide from Ganoderma atrum on intestinal mucosal immunity and mucosal injury in mice[D]. Nanchang:Nanchang University, 2018.(in Chinese)
[21]	LIU C, LUO J, XUE R Y, et al. The mucosal adjuvant effect of plant polysaccharides for induction of protective immunity against Helicobacter pylori infection[J]. Vaccine, 2019, 37(8):1053-1061.
[22]	谭西,周欣,陈华国.多糖结构修饰研究进展[J].食品工业科技, 2019, 40(4):341-349, 356.TAN X, ZHOU X, CHEN H G. Research progress on structural modification of polysaccharides[J]. Science and Technology of Food Industry, 2019, 40(4):341-349, 356.(in Chinese)
[23]	HUANG G L, HUANG H L. The derivatization and antitumor mechanisms of polysaccharides[J]. Future Med Chem, 2017, 9(16):1931-1938.
[24]	CHENG L Z, WANG Y F, HE X X, et al. Preparation, structural characterization and bioactivities of Se-containing polysaccharide:a review[J]. Int J Biol Macromol, 2018, 120:82-92.
[25]	BO R N, JI X, YANG H F, et al. The characterization of optimal selenized garlic polysaccharides and its immune and antioxidant activity in chickens[J]. Int J Biol Macromol, 2021, 182:136-143.
[26]	金清.黄芪多糖协同硫酸化淫羊藿多糖增强仔猪免疫的作用研究[D].荆州:长江大学, 2020.JIN Q. Astragalus polysaccharides synergize sulfated epimedium polysaccharides to enhance piglet immunity[D]. Jingzhou:Yangtze University, 2020.(in Chinese)
[27]	DEWANGAN H K. Rational application of nanoadjuvant for mucosal vaccine delivery system[J]. J Immunol Methods, 2020, 481-482:112791.
[28]	KANEKO K, MCDOWELL A, ISHII Y, et al. Liposomal α-galactosylceramide is taken up by gut-associated lymphoid tissue and stimulates local and systemic immune responses[J]. J Pharm Pharmacol, 2017, 69(12):1724-1735.
[29]	周兆海,梁浩钊,陈刚,等.玉屏风多糖脂质体对雏鸡黏膜免疫的影响[J].动物医学进展, 2019, 40(10):54-59.ZHOU Z H, LIANG H Z, CHEN G, et al. Effect of Yupingfeng polysaccharide liposomes on mucosal immunity of chicks[J]. Progress in Veterinary Medicine, 2019, 40(10):54-59.(in Chinese)
[30]	梁浩钊,吴春琼,李樵锋,等.玉屏风多糖脂质体对雏鸡肠黏膜及淋巴组织形态结构的影响[J].动物医学进展, 2020, 41(11):84-89.LIANG H Z, WU C Q, LI Q F, et al. Effect of yupingfeng polysaccharides liposomes on morphology and structure of intestinal mucosa-associated lymphoid tissue in chicks[J]. Progress in Veterinary Medicine, 2020, 41(11):84-89.(in Chinese)
[31]	SINGH B, MAHARJAN S, CHO K H, et al. Chitosan-based particulate systems for the delivery of mucosal vaccines against infectious diseases[J]. Int J Biol Macromol, 2018, 110:54-64.
[32]	MEHRABI M, MONTAZERI H, DOUNIGHI N M, et al. Chitosan-based nanoparticles in mucosal vaccine delivery[J]. Arch Razi Inst, 2018, 73(3):165-176.
[33]	LI D, FU D W, KANG H, et al. Advances and potential applications of chitosan nanoparticles as a delivery carrier for the mucosal immunity of vaccine[J]. Curr Drug Deliv, 2017, 14(1):27-35.
[34]	KUMAR A, VIMAL A, KUMAR A. Why Chitosan?From properties to perspective of mucosal drug delivery[J]. Int J Biol Macromol, 2016, 91:615-622.
[35]	KOUR P, RATH G, SHARMA G, et al. Recent advancement in nanocarriers for oral vaccination[J]. Artif Cells, Nanomed, Biotechnol, 2018, 46(S3):S1102-S1114.
[36]	史同瑞,刘宇,王爽,等.现代中药发酵技术及其优势[J].中兽医学杂志, 2014(1):51-54.SHI T R, LIU Y, WANG S, et al. Modern Chinese medicine fermentation technology and its advantages[J]. Chinese Journal of Traditional Veterinary Science, 2014(1):51-54.(in Chinese)
[37]	梁子敬.发酵黄芪多糖的结构表征及其对小鼠肠道黏膜免疫的改善作用[D].北京:中国农业科学院, 2019.LIANG Z J. Structural characterization of fermented Astragalus membranaceus polysaccharides and its improvement effect on intestinal mucosal immunity in mice[D]. Beijing:Chinese Academy of Agricultural Sciences, 2019.(in Chinese)
[38]	LI S, WANG X F, REN L N, et al. Protective effects of γ-irradiated Astragalus polysaccharides on intestinal development and mucosal immune function of immunosuppressed broilers[J]. Poult Sci, 2019, 98(12):6400-6410.
[39]	CAMILLERI M, MADSEN K, SPILLER R, et al. Intestinal barrier function in health and gastrointestinal disease[J]. Neurogastroenterol Motil, 2012, 24(6):503-512.
[40]	李任军,王海梅,胡松华.补益类中药对动物肠黏膜免疫的调节作用[J].中兽医医药杂志, 2019, 38(4):26-28.LI R J, WANG H M, HU S H. Reviewed on immunoregulation of traditional Chinese medicine with tonifying effect on animal intestinal mucosal immune[J]. Journal of Traditional Chinese Veterinary Medicine, 2019, 38(4):26-28.(in Chinese)
[41]	GEHART H, CLEVERS H. Tales from the crypt:new insights into intestinal stem cells[J]. Nat Rev Gastroenterol Hepatol, 2019, 16(1):19-34.
[42]	SEIKRIT C, PABST O. The immune landscape of IgA induction in the gut[J]. Semin Immunopathol, 2021, 43(5):627-637.
[43]	王翌,李淼,孙元,等.靶向M细胞的抗原递送——增强黏膜免疫应答的关键策略[J].生物工程学报, 2019, 35(2):216-225.WANG Y, LI M, SUN Y, et al. Microfold cells-targeting antigen delivery:a promising strategy to enhance the efficacy of mucosal vaccines[J]. Chinese Journal of Biotechnology, 2019, 35(2):216-225.(in Chinese)
[44]	OH S H, KIM S H, JEON J H, et al. Cytoplasmic expression of a model antigen with M Cell-Targeting moiety in lactic acid bacteria and implication of the mechanism as a mucosal vaccine via oral route[J]. Vaccine, 2021, 39(30):4072-4081.
[45]	KOMBAN R J, STRÖMBERG A, BIRAM A, et al. Activated Peyer's patch B cells sample antigen directly from M cells in the subepithelial dome[J]. Nat Commun, 2019, 10(1):2423.
[46]	JIANG Y P, LI X L, WU Y, et al. Effect of Lentinan on Peyer's patch structure and function in an immunosuppressed mouse model[J]. Int J Biol Macromol, 2019, 137:169-176.
[47]	XIE S Z, LIU B, YE H Y, et al. Dendrobium huoshanense polysaccharide regionally regulates intestinal mucosal barrier function and intestinal microbiota in mice[J]. Carbohydr Polym, 2019, 206:149-162.
[48]	JIN M L, ZHU Y M, SHAO D Y, et al. Effects of polysaccharide from mycelia of Ganoderma lucidum on intestinal barrier functions of rats[J]. Int J Biol Macromol, 2017, 94:1-9.
[49]	YING M X, ZHENG B, YU Q, et al. Ganoderma atrum polysaccharide ameliorates intestinal mucosal dysfunction associated with autophagy in immunosuppressed mice[J]. Food Chem Toxicol, 2020, 138:111244.
[50]	PIETRZAK B, TOMELA K, OLEJNIK-SCHMIDT A, et al. Secretory IgA in intestinal mucosal secretions as an adaptive barrier against microbial cells[J]. Int J Mol Sci, 2020, 21(23):9254.
[51]	LI Y, JIN L, CHEN T X. The effects of secretory IgA in the mucosal immune system[J]. Biomed Res Int, 2020, 2020:2032057.
[52]	GOMMERMAN J L, ROJAS O L, FRITZ J H. Re-thinking the functions of IgA+ plasma cells[J]. Gut Microbes, 2014, 5(5):652-662.
[53]	KAETZEL C S. The polymeric immunoglobulin receptor:bridging innate and adaptive immune responses at mucosal surfaces[J]. Immunol Rev, 2005, 206(1):83-99.
[54]	JOHANSEN F E, KAETZEL C S. Regulation of the polymeric immunoglobulin receptor and IgA transport:new advances in environmental factors that stimulate pIgR expression and its role in mucosal immunity[J]. Mucosal Immunol, 2011, 4(6):598-602.
[55]	TURULA H, WOBUS C E. The role of the polymeric immunoglobulin receptor and secretory immunoglobulins during mucosal infection and immunity[J]. Viruses, 2018, 10(5):237.
[56]	SHAN C L, SUN B D, DALLOUL R A, et al. Effect of the oral administration of astragalus polysaccharides on jejunum mucosal immunity in chickens vaccinated against Newcastle disease[J]. Microb Pathog, 2019, 135:103621.
[57]	BAI Y J, HUANG F, ZHANG R F, et al. Longan pulp polysaccharide protects against cyclophosphamide-induced immunosuppression in mice by promoting intestinal secretory IgA synthesis[J]. Food Funct, 2020, 11(3):2738-2748.
[58]	谢天宇,胡红莲,高民.肠黏膜免疫屏障及其保护措施[J].动物营养学报, 2014, 26(5):1157-1163.XIE T Y, HU H L, GAO M. Gut mucosal immune barrier and the protective measures[J]. Chinese Journal of Animal Nutrition, 2014, 26(5):1157-1163.(in Chinese)
[59]	丘富安,任喆,郑纪元,等.参芪多糖口服液对鸡空肠黏膜免疫功能的保护作用[J].中国兽医科学, 2017, 47(11):1441-1449.QIU F A, REN Z, ZHENG J Y, et al. Protective effects of Shenqi polysaccharide oral liquid on jejunal mucosal immunity in chickens[J]. Chinese Veterinary Science, 2017, 47(11):1441-1449.(in Chinese)
[60]	NEURATH M F, FINOTTO S, GLIMCHER L H. The role of Th1/Th2 polarization in mucosal immunity[J]. Nat Med, 2002, 8(6):567-573.
[61]	VAN WIJK F, CHEROUTRE H. Intestinal T cells:facing the mucosal immune dilemma with synergy and diversity[J]. Semin Immunol, 2009, 21(3):130-138.
[62]	OLIVARES-VILLAGÓMEZ D, VAN KAER L. Intestinal intraepithelial lymphocytes:sentinels of the mucosal barrier[J]. Trends Immunol, 2018, 39(4):264-275.
[63]	陈凌锋,蔡旭滨,檀新珠,等.太子参茎叶多糖对断奶仔猪肠道免疫功能、肠黏膜形态结构及盲肠内容物菌群的影响[J].动物营养学报, 2017, 29(3):1012-1020.CHEN L F, CAI X B, TAN X Z, et al. Effects of Radix pseudostellariae stem and leaf polysaccharide on intestinal immune function, intestinal mucosal morphology and cecum contents flora of weaned piglets[J]. Chinese Journal of Animal Nutrition, 2017, 29(3):1012-1020.(in Chinese)
[64]	牛祥云.松花粉多糖对小鼠肠道微环境及溃疡性结肠炎的作用研究[D].泰安:山东农业大学, 2021.NIU X Y. Effects of Pinus Pollen Polysaccharides on Intestinal Microenvironment and Colitis in Mice[D]. Taian:Shandong Agricultural University, 2021.(in Chinese)
[65]	沙洲.松花粉多糖对鸡肠道黏膜免疫的影响及巨噬细胞的免疫调节作用[D].泰安:山东农业大学, 2021.SHA Z. Effects of pine pollen polysaccharides on intestinal mucosal immunity of chickens and immunomodulatory of macrophages[D]. Taian:Shandong Agricultural University, 2021.(in Chinese)
[66]	BEKIARIS V, PERSSON E K, AGACE W W. Intestinal dendritic cells in the regulation of mucosal immunity[J]. Immunol Rev, 2014, 260(1):86-101.
[67]	IWASAKI A. Mucosal dendritic cells[J]. Annu Rev Immunol, 2007, 25:381-418.
[68]	SOLOFF A C, BARRATT-BOYES S M. Enemy at the gates:dendritic cells and immunity to mucosal pathogens[J]. Cell Res, 2010, 20(8):872-885.
[69]	鹿秀云,岳海洋,刘億,等.黄芪多糖对结肠炎小鼠树突状细胞表面共刺激分子表达的调节作用[J].中成药, 2018, 40(10):2296-2298.LU X Y, YUE H Y, LIU Y, et al. Effects of Astragalus polysaccharide on the expression of costimulatory molecules in dendritic cells of colitis mice[J]. Chinese Traditional Patent Medicine, 2018, 40(10):2296-2298.(in Chinese)
[70]	ZHAO H M, WANG Y, HUANG X Y, et al. Astragalus polysaccharide attenuates rat experimental colitis by inducing regulatory T cells in intestinal Peyer's patches[J]. World J Gastroenterol, 2016, 22(11):3175-3185.
[71]	CHEN K, MAGRI G, GRASSET E K, et al. Rethinking mucosal antibody responses:IgM, IgG and IgD join IgA[J]. Nat Rev Immunol, 2020, 20(7):427-441.
[72]	CHEN Q Q, REN R R, ZHANG Q Q, et al. Coptis chinensis Franch polysaccharides provide a dynamically regulation on intestinal microenvironment, based on the intestinal flora and mucosal immunity[J]. J Ethnopharmacol, 2021, 267:113542.
[73]	YING M X, YU Q, ZHENG B, et al. Cultured Cordyceps sinensis polysaccharides modulate intestinal mucosal immunity and gut microbiota in cyclophosphamide-treated mice[J]. Carbohydr Polym, 2020, 235:115957.
[74]	陶未来,刘佳,刘琼丹,等.术苦芩总多糖对湿热泄泻仔猪肠道菌群和免疫功能的影响[J].畜牧兽医学报, 2022, 53(3):913-924.TAO W L, LIU J, LIU Q D, et al. Effects of total polysaccharides from Zhukuqin on intestinal flora and immune function in piglets with dampness-heat diarrhea[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(3):913-924.(in Chinese)
[75]	HUANG C, CAO X Y, CHEN X F, et al. A pectic polysaccharide from Ligusticum chuanxiong promotes intestine antioxidant defense in aged mice[J]. Carbohydr Polym, 2017, 174:915-922.

Research Progress of Chinese Herbal Polysaccharides as Immunopotentiator and Its Effect on Intestinal Mucosa

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