1株C3样奇异变形杆菌烈性噬菌体的生物学特性及基因组分析

doi:10.11843/j.issn.0366-6964.2021.04.022

Abstract

Abstract: This study was conducted to isolate and characterize a lytic bacteriophage against Proteus mirabilis from piglet fecal samples. Bacteriophage was isolated and purified with indicator of Proteus mirabilis by double-layer agar plate method from fecal samples of piglets. The size and shape of phage was observed with transmission electron microscopy. The whole genome of phage was extracted and sequenced by Illumina Hiseq high-throughput sequencing technology, and then the genome was annotated and analyzed. A lytic phage (designated as vB_PmiP_P59) against Proteus mirabilis was successfully isolated. P59 was a podovirus with C3 morphotype: a flattened oval head of 152 nm×54 nm and a tail of 40 nm×12 nm. The optimal MOI of P59 was 0.001.The one-step growth curve showed that P59 had a latent period of 30 min, a burst period of 105 min, and a burst size of 2 754 PFU·cell^-1. The phage could withstand the temperature up to 50 ℃ and maintain stable titer under pH 5-10.The double-stranded DNA genome was 90 187 bp long with a mol% G+C content of 34.65, and contained 136 ORFs and 4 tRNAs without any virulent or antibiotic resistance genes. Whole-genome proteomic analysis (30.47% homologous proteins) revealed that it was related to C3-like phage phiEco32. A novel lytic Proteus mirabilis phage vB_PmiP_P59 is identified with significant ability to inhibit the growth of Proteus mirabilis.

Key words: Proteus mirabilis, bacteriophage, C3, biological characterization, genomic analysis

CLC Number:

Q939.48

LI Shuya, HUANG Zan, ZHU Weiyun, LIN Yan. Biological Characteristics and Genomic Analysis of a Lytic Proteus mirabilis Phage with C3 Morphotype[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 1069-1078.

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Biological Characteristics and Genomic Analysis of a Lytic Proteus mirabilis Phage with C3 Morphotype

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