捻转血矛线虫重组磷脂酰肌醇转移蛋白对山羊外周血单个核细胞模式识别受体和细胞因子转录水平的影响

doi:10.11843/j.issn.0366-6964.2023.01.023

Abstract

Abstract: To study the effects of recombinant phosphatidylinositol transfer protein (HCPITP) from Haemonchus contortus on goat immunity, prokaryotic expression recombinant plasmid pET-28a-HCPITP was constructed and the recombinant protein was obtained. The differences of transcription level of HCPITP gene in egg, the third-stage larvae, female and male adult worm were analyzed. Goat peripheral blood mononuclear cells (PBMCs) were isolated and incubated with recombinant HCPITP, the effects of recombinant protein on the proliferation of goat PBMCs, the effects of recombinant protein on the transcription levels of pattern recognition receptors (PRRs) and cytokines in goat PBMCs were analyzed. Results showed that the relative transcription levels of HCPITP mRNA in female, male, the third-stage larvae, and egg were 1, 10.2, 0.47 and 0.58, respectively. The recombinant protein showed reactogenicity by Western blot analysis. It was found that recombinant HCPITP significantly inhibited the proliferation of ConA on PBMCs. Recombinant HCPITP promoted the transcription of IL-2, IL-4, IL-6, IL-17, TLR1 and TLR10 in goat PBMCs. In conclusion, the transcription level of HCPITP gene shows obvious stage differences, recombinant HCPITP has a certain effect on the proliferation and mRNA transcription of PRRs and cytokines of goat PBMCs.

Key words: Haemonchus contortus, phosphatidylinositol transfer protein, pattern recognition receptors, immunomodulation

CLC Number:

S852.73

XIE Xinran, ZHANG Yue, LU Mingmin, XU Lixin, SONG Xiaokai, LI Xiangrui, YAN Ruofeng. Effects of Recombinant Phosphatidylinositol Transfer Protein from Haemonchus contortus on the Transcription of Pattern Recognition Receptors and Cytokines in Goat Peripheral Blood Mononuclear Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 252-262.

References 35

[1]	ALI R, ROOMAN M, MUSSARAT S, et al. A systematic review on comparative analysis, toxicology, and pharmacology of medicinal plants against Haemonchus contortus[J]. Front Pharmacol, 2021, 12:644027.
[2]	ZENEBE S, FEYERA T, ASSEFA S. In vitro anthelmintic activity of crude extracts of aerial parts of Cissus quadrangularis L. and leaves of Schinus molle L. against Haemonchus contortus[J]. Biomed Res Int, 2017, 2017:1905987.
[3]	HSUAN J, COCKCROFT S. The PITP family of phosphatidylinositol transfer proteins[J]. Genome Biol, 2001, 2(9):REVIEWS3011.
[4]	COCKCROFT S, GARNER K. Potential role for phosphatidylinositol transfer protein (PITP) family in lipid transfer during phospholipase C signalling[J]. Adv Biol Regul, 2013, 53(3):280-291.
[5]	MICHELL R H. Inositol phospholipids and cell surface receptor function[J]. Biochim Biophys Acta, 1975, 415(1):81-147.
[6]	THOMAS G M H, CUNNINGHAM E, FENSOME A, et al. An essential role for phosphatidylinositol transfer protein in phospholipase C-mediated inositol lipid signaling[J]. Cell, 1993, 74(5):919-928.
[7]	KAUFFMANN-ZEH A, THOMAS G M H, BALL A, et al. Requirement for phosphatidylinositol transfer protein in epidermal growth factor signaling[J]. Science, 1995, 268(5214):1188-1190.
[8]	HAY J C, FISETTE P L, JENKINS G H, et al. ATP-dependent inositide phosphorylation required for Ca2+-activated secretion[J]. Nature, 1995, 374(6518):173-177.
[9]	FENSOME A, CUNNINGHAM E, PROSSER S, et al. ARF and PITP restore GTPγS-stimulated protein secretion from cytosol-depleted HL60 cells by promoting PIP2 synthesis[J]. Curr Biol, 1996, 6(6):730-738.
[10]	HAY J C, MARTIN T F J. Phosphatidylinositol transfer protein required for ATP-dependent priming of Ca2+-activated secretion[J]. Nature, 1993, 366(6455):572-575.
[11]	IWATA R, ODA S, KUNITOMO H, et al. Roles for class IIA phosphatidylinositol transfer protein in neurotransmission and behavioral plasticity at the sensory neuron synapses of Caenorhabditis elegans[J]. Proc Natl Acad Sci U S A, 2011, 108(18):7589-7594.
[12]	ABERGEL Z, SHAKED M, SHUKLA V, et al. The phosphatidylinositol transfer protein PITP-1 facilitates fast recovery of eating behavior after hypoxia in the nematode Caenorhabditis elegans[J]. FASEB J, 2021, 35(1):e21202.
[13]	CHAPARRO J J, VILLAR D, ZAPATA J D, et al. Multi-drug resistant Haemonchus contortus in a sheep flock in Antioquia, Colombia[J]. Vet Parasitol Reg Stud Reports, 2017, 10:29-34.
[14]	DOS SANTOS J M L, VASCONCELOS J F, FROTA G A, et al. Quantitative molecular diagnosis of levamisole resistance in populations of Haemonchus contortus[J]. Exp Parasitol, 2019, 205:107734.
[15]	DUARTE E R, MATIAS A D, BASTOS G A, et al. Anthelmintic efficacy of trichlorfon and blood parameters of young lambs infected with Haemonchus contortus[J]. Vet Parasitol, 2019, 272:40-43.
[16]	WANG W, WANG S, ZHANG H, et al. Galectin Hco-gal-m from Haemonchus contortus modulates goat monocytes and T cell function in different patterns[J]. Parasit Vectors, 2014, 7:342.
[17]	EHSAN M, GADAHI J A, LU M M, et al. Recombinant elongation factor 1 alpha of Haemonchus contortus affects the functions of goat PBMCs[J]. Parasite Immunol, 2020, 42(5):e12703.
[18]	WEN Z H, ALEEM M T, AIMULAJIANG K, et al. The GT1-TPS structural domain protein from Haemonchus contortus could Be suppressive antigen of goat PBMCs[J]. Front Immunol, 2022, 12:787091.
[19]	MOLL H. Dendritic cells and host resistance to infection[J]. Cell Microbiol, 2003, 5(8):493-500.
[20]	JACOBS H J, ASHMAN K, MEEUSEN E. Humoral and cellular responses following local immunization with a surface antigen of the gastrointestinal parasite Haemonchus contortus[J]. Vet Immunol Immunopathol, 1995, 48(3-4):323-332.
[21]	GILL H S. Cell-mediated immunity in merino lambs with genetic resistance to Haemonchus contortus[J]. Int J Parasitol, 1994, 24(5):749-756.
[22]	KAWAI T, AKIRA S. Toll-like receptors and their crosstalk with other innate receptors in infection and immunity[J]. Immunity, 2011, 34(5):637-650.
[23]	REHWINKEL J, GACK M U. RIG-I-like receptors:their regulation and roles in RNA sensing[J]. Nat Rev Immunol, 2020, 20(9):537-551.
[24]	DROUIN M, SAENZ J, CHIFFOLEAU E. C-type lectin-like receptors:head or tail in cell death immunity[J]. Front Immunol, 2020, 11:251.
[25]	DUXBURY Z, WU C H, DING P T. A Comparative overview of the intracellular guardians of plants and animals:NLRs in innate immunity and beyond[J]. Annu Rev Plant Biol, 2021, 72:155-184.
[26]	HAYASHI F, SMITH K D, OZINSKY A, et al. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5[J]. Nature, 2001, 410(6832):1099-1103.
[27]	YIN F Y, LIU J L, GAO S D, et al. Exploring the TLR and NLR signaling pathway relevant molecules induced by the Theileria annulata infection in calves[J]. Parasitol Res, 2018, 117(10):3269-3276.
[28]	SIMONE O, BEJARANO M T, PIERCE S K, et al. TLRs innate immunereceptors and Plasmodium falciparum erythrocyte membrane protein 1(PfEMP1) CIDR1α-driven human polyclonal B-cell activation[J]. Acta Trop, 2011, 119(2-3):144-150.
[29]	ZHANG F K, HOU J L, GUO A J, et al. Expression profiles of genes involved in TLRs and NLRs signaling pathways of water buffaloes infected with Fasciola gigantica[J]. Mol Immunol, 2018, 94:18-26.
[30]	RAPHAEL I, NALAWADE S, EAGAR T N, et al. T cell subsets and their signature cytokines in autoimmune and inflammatory diseases[J]. Cytokine, 2015, 74(1):5-17.
[31]	O'SHEA J J, PAUL W E. Mechanisms underlying lineage commitment and plasticity of helper CD4+ T cells[J]. Science, 2010, 327(5969):1098-1102.
[32]	THORNTON A M, SHEVACH E M. Suppressor effector function of CD4+CD25+ immunoregulatory T cells is antigen nonspecific[J]. J Immunol, 2000, 164(1):183-190.
[33]	SHEVACH E M. CD4+CD25+ suppressor T cells:more questions than answers[J]. Nat Rev Immunol, 2002, 2(6):389-400.
[34]	LIAO W, LIN J X, LEONARD W J. IL-2 family cytokines:new insights into the complex roles of IL-2 as a broad regulator of T helper cell differentiation[J]. Curr Opin Immunol, 2011, 23(5):598-604.
[35]	LIAO W, LIN J X, WANG L, et al. Modulation of cytokine receptors by IL-2 broadly regulates differentiation into helper T cell lineages[J]. Nat Immunol, 2011, 12(6):551-559.

Effects of Recombinant Phosphatidylinositol Transfer Protein from Haemonchus contortus on the Transcription of Pattern Recognition Receptors and Cytokines in Goat Peripheral Blood Mononuclear Cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 35

Related Articles 8

Recommended Articles

Metrics

Comments

[1]	GONG Haoyang, WU Jiaxin, YANG Xiaoyu, XIE Weichun, WANG Xueying, LI Jiaxuan, JIANG Yanping, CUI Wen, LI Yijing, TANG Lijie. Research Progress in Antiviral Mechanism of Intestinal Microflora [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4910-4919.
[2]	CUI Enhui, XUE Yuhuan, LI Cixia, WANG Shuai, ZHU Xiaoyan, CHAI Xuejun, ZHAO Shanting. Network Pharmacological Analysis and Experimental Verification of Immunomodulatory Mechanisms of Eucommia ulmoides Leaf [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 403-413.
[3]	LIANG Gaoxing, RONG Shiqi, WANG Junwei, LI Yuan, YANG Xin, ZHAO Guanghui, SONG Junke. Multi-locus Gene Sequence Analysis of Female Haemonchus contortus with Morphological Differences in Vulval Flap from Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3140-3148.
[4]	YU Ruihong, MENG Zhen, SUN Mengke, CHEN Shixiong, ZHANG Junwen, HUANG Yifan, QIN Tao, REN Zhe. Structural Characterization and Immunomodulatory Activities of Polysaccharide Extracted from Lagotis brevituba Maxim [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3270-3281.
[5]	SHANG Lijun, YANG Tianren, YU Haitao, HUANG Shuo, ZENG Xiangfang, QIAO Shiyan. Immunomodulation Effect of Antimicrobial Peptide Sublancin and Astragalus Polysaccharides on Immunosuppressed Mice: A Comparative Study [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 406-414.
[6]	ZHAO Qi-ling，ZHANG Xin-chen，CHEN Meng-meng，SUN Jia-rui，BAO En-dong，ZHANG Shu-xia，Lü Ying-jun. Regulation of Interferon Signaling Pathway in Alveolar Macrophages of Piglets Infected with Procine Circovirus Type 2 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 587-594.
[7]	JING Zhi-zhong;HE Xiao-bing;FANG Yong-xiang;CHEN Guo-hua;ZENG Shuang;JIA Huai-jie . Progress on the Molecular Patterns and Pathways of Host Recognitionto Viral Nucleic Acids [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2011, 42(3): 311-322.
[8]	HAN Chun-yang;LIN De-gui;LIU Cui-yan;GUO Shun-xing. Immunomodulation and Antitumor Activity of Fermented Decoction of Sijunzi in S180-bearing Mice [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2008, 39(6): 814-818.