马齿苋水提物对热应激小鼠空肠结构及吸收功能的影响

doi:10.11843/j.issn.0366-6964.2020.04.024

Abstract

Abstract: The present study was conducted to investigate the effects of aqueous extract of Portulaca oleracea L. (AEP) on the structure and function of jejunum in mouse under the heat stress conditions. A total of 40 Kunming mice were randomly allocated to 4 groups:control group (C), heat stress group (HS), aqueous extract of Portulaca oleracea L. (AEP), and vitamin C group (Vc), heat stress (in groupHS, AEP &Vc) was conducted by exposing mice to (40±1)℃ for 0.5 h, and after 6 days of continuous heat stress, the mice were transferred to room temperature for treatment. Seven days after the administration, blood samples were collected from the orbital sinus and the jejunum of the mice was collected. The contents of D-xylose and glucose in the serum of mice were measured; the pathological changes of jejunum were observed by HE staining; qRT-PCR was used to detect the relative transcription levels of ZO-1, SGLT1 and GLUT2 mRNA in the jejunal mucosa. The results showed that compared with group C, the jejunal mucosal villi height of the HS group mice was significantly reduced (P<0.01); the level of D-xylose in the serum were significantly reduced (P<0.01); the relative transcription levels of ZO-1, SGLT1 and GLUT2 mRNA in the jejunal mucosa were significantly reduced (P<0.01); compared with the HS group mice, the jejunum villi of the AEP group mice were significantly increased (P<0.01); the levels of D-xylose and glucose in serum were significantly increased (P <0.01 or P<0.05); the relative transcription levels of ZO-1, SGLT1 and GLUT2 mRNA in the jejunal mucosa were significantly increased (P<0.05 or P<0.01). High temperature treatment can induce the jejunum mucosa to fall off and decrease the absorption function. After giving purslane extract treatment, the jejunum mucosa structure can be repaired by increasing the jejunum ZO-1 mRNA transcription, and absorption functioncan be improved by promoting the jejunum mucosa SGLT1 and GLUT2 mRNA transcription. AEP can repair the jejunum mucosa damage caused by heat stress to a certain level, and improve the intestinal absorption capacity of heat stress mice.

Key words: heat stress, mouse, aqueous extract of Portulaca oleracea L., jejunal structure, jejunal absorption

CLC Number:

S853.7

GUO Yue, LIU Xiaobao, LI Jian, HE Xin, ZHAO Xinghua. The Effects of Aqueous Extract of Portulaca oleracea L.on the Structure and Function of Jejunum in Mice with Heat Stress[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 873-880.

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The Effects of Aqueous Extract of Portulaca oleracea L.on the Structure and Function of Jejunum in Mice with Heat Stress

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