小鼠黑色素细胞沉默及过表达色素上皮衍生因子对黑色素合成的影响

doi:10.11843/j.issn.0366-6964.2023.09.030

Abstract

Abstract: The purpose of this study was to investigate the interaction between pigment epithelium-derived factor (PEDF) and melanin synthesis regulatory factors, and to explore the regulatory effects of PEDF on melanin cell vitality and melanin synthesis, so as to provide a basis for the study of animal coat color formation mechanism. In this study, PEDF siRNA and PEDF overexpression vector were transfected into mouse melanocytes respectively, and the activity of melanocytes and content of melanin were detected. The expression and localization of PEDF, Wnt1, Wnt3α, MITF and β-catenin were detected by qRT-PCR, Western blotting and cellular immunofluorescence. After PEDF silencing, the cell vitality of melanocytes was enhanced, the content of melanin was increased, and the mRNA and protein expressions of Wnt1, Wnt3α, MITF and β-catenin were increased. After PEDF overexpression in melanocytes, the activity of melanocytes was weakened, the content of melanin was decreased, and the mRNA and protein expressions of Wnt1, Wnt3α, MITF and β-catenin were decreased. Cellular immunofluorescence results showed that PEDF, Wnt1, MITF and β-catenin were expressed in the cytoplasm of melanocytes. Wnt3α was expressed in the cytoplasm and cell membrane of melanocytes. This study could confirm that PEDF could reduce the activity of melanocytes, and inhibit the production of melanin by down-regulating the factors related to melanin production, Wnt1, Wnt3α, MITF and β-catenin.

Key words: pigment epithelium-derived factor, PEDF, silence and overexpression, melanin, mouse melanocyte

CLC Number:

Q342

LIANG Rui, FAN Xiaorui, ZHANG Jinqiang, PANG Quanhai. Effects of Mouse Melanocyte Silencing and Overexpression of Pigment Epithelium-Derived Factor on Melanin Synthesis[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3916-3930.

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Effects of Mouse Melanocyte Silencing and Overexpression of Pigment Epithelium-Derived Factor on Melanin Synthesis

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