白藜芦醇对急性热应激条件下鸭肝抗氧化能力和细胞凋亡的影响

doi:10.11843/j.issn.0366-6964.2023.01.022

Abstract

Abstract: This study was conducted to explore the effects of dietary resveratrol supplementation on liver anti-oxidant capacity and anti-apoptosis of Shanma ducks under acute heat stress, and to determine the effects of resveratrol supplementation on SIRT1 signaling pathway, antioxidant enzyme system and apoptosis related genes in the liver of Shanma ducks. A total of 120 healthy female Shanma ducks with similar weight at 60 days old were randomly divided into two groups, 60 in each group. The control (C) group was fed a basic diet, and the resveratrol (RES) group was fed a basic diet supplemented with 400 mg·kg^-1 resveratrol. Animals in two groups were kept at a temperature of (24±2) ℃. After 15 days, there was no significant difference in the weight of the animals between the two groups. Then the animals in each group were randomly divided into 3 groups,20 in each group, and placed in an artificial climate room at 39 ℃ for 0, 30, and 60 min, respectively. After heat stress treatment, duck serum samples and liver tissues were collected, and the mRNA, protein level and apoptosis number of SIRT1 signal pathway and apoptosis related genes were detected using qRT-PCR, Western blot and TUNEL techniques. Biochemical method was adopted to detect the oxidation index in serum and liver. The results showed that the mRNA expression of HSP70and HSP90 in RES group was higher than those in control group. At the same time, resveratrol activated the SIRT1 pathway and increased the mRNA expressions of downstream PGC-1α and TFAM, and significantly increased the nuclear proteins expression of Nrf1 and Nrf2 under heat stress for 60 min. Resveratrol increased the levels of T-AOC in serum. Resveratrol significantly increased the levels of SOD, CAT, AIHR and decreased the content of MDA under heat stress for 60 min，and under the same heat stress condition， the T-AOC, CAT and GSH-Px in liver of RES group were higher than those of C group, while MDA content in RES group was lower than that in C group. Under the condition of acute heat stress, the number of hepatocyte apoptosis in RES group was significantly lower than that in C group, and resveratrol decreased the mRNA expression of Bax, Bak1, Bax/Bcl-2, increased the protein expression of Bcl-2, and decreased the protein expression of Bax, Caspase3, Bax/Bcl-2. In conclusion, the addition of 400 mg·kg^-1 resveratrol to the diet can activate the SIRT1 signaling pathway in duck liver, reduce the expression of pro-apoptotic genes and increase the expression of anti-apoptotic genes. Resveratrol can improve the anti-oxidant capacity and anti-apoptosis ability of duck liver, and can improve the oxidative damage of duck liver caused by acute heat stress.

Key words: resveratrol, heat stress, antioxidant, apoptosis

CLC Number:

S834.4

ZHOU Wanting, YANG Chen, PENG Cuitian, FU Xinliang, ZHONG Zhuohua, XU Danning, HUANG Yunmao, TIAN Yunbo, LIU Wenjun. Effects of Resveratrol on Anti-oxidation and Anti-apoptosis of Hepatocytes of Ducks on Exposure to Acute Heat Stress[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 239-251.

References 41

[1]	ROUSHDY E M,ZAGLOOL A W,HASSAN F A M.Thermal stress consequences on growth performance,immunological response,antioxidant status,and profitability of finishing broilers:transcriptomic profile change of stress-related genes[J].Trop Anim Health Prod,2020,52(6):3685-3696.
[2]	WOLC A,ARANGO J,SETTAR P,et al.Genome wide association study for heat stress induced mortality in a white egg layer line[J].Poult Sci,2019,98(1):92-96.
[3]	LALIOTIS G P,BIZELIS I,ROGDAKIS E.Comparative approach of the de novo fatty acid synthesis (Lipogenesis) between ruminant and non ruminant mammalian species:From biochemical level to the main regulatory lipogenic genes[J].Curr Genomics,2010,11(3):168-183.
[4]	DING H R,WANG J L,REN H Z,et al.Lipometabolism and glycometabolism in liver diseases[J].BioMed Res Int,2018,2018:1287127.
[5]	EMAMI N K,JUNG U,VOY B,et al.Radical response:Effects of heat stress-induced oxidative stress on lipid metabolism in the avian liver[J].Antioxidants (Basel),2020,10(1):35.
[6]	XIE J J,TANG L,LU L,et al.Differential expression of heat shock transcription factors and heat shock proteins after acute and chronic heat stress in laying chickens (Gallus gallus)[J].PLoS One,2014,9(7):e102204.
[7]	ABDO S E,EL-KASSAS S,EL-NAHAS A F,et al.Modulatory effect of monochromatic blue light on heat stress response in commercial broilers[J].Oxid Med Cell Longev,2017,2017:1351945.
[8]	LU Z,HE X F,MA B B,et al.Increased fat synthesis and limited apolipoprotein B cause lipid accumulation in the liver of broiler chickens exposed to chronic heat stress[J].Poult Sci,2019,98(9):3695-3704.
[9]	YANG L,TAN G Y,FU Y Q,et al.Effects of acute heat stress and subsequent stress removal on function of hepatic mitochondrial respiration,ROS production and lipid peroxidation in broiler chickens[J].Comp Biochem Physiol C Toxicol Pharmacol,2010, 151(2):204-208.
[10]	马政,亢守亭,蔡锋隆,等.白藜芦醇对肉鸡生长性能、血清生化指标和抗氧化能力的影响[J].动物营养学报,2022,34(3):1559-1568.MA Z,KANG S T,CAI F L,et al.Effects of resveratrol on growth performance,serum biochemical indexes and antioxidant capacity of broilers[J].Chinese Journal of Animal Nutrition,2022,34(3):1559-1568.(in Chinese)
[11]	FARGHALI H,KEMELO M K,CANOV N K.SIRT1 modulators in experimentally induced liver injury[J].Oxid Med Cell Longev,2019,2019:8765954.
[12]	CHEN Y P,CHENG Y F,WEN C,et al.Protective effects of dietary mannan oligosaccharide on heat stress-induced hepatic damage in broilers[J].Environ Sci Pollut Res,2020,27(23):29000-29008.
[13]	AKBARIAN A,MICHIELS J,DEGROOTE J,et al.Association between heat stress and oxidative stress in poultry;Mitochondrial dysfunction and dietary interventions with phytochemicals[J].J Anim Sci Biotechnol,2016,7(1):37.
[14]	张宇,徐子洁,黄晓瑜,等.白藜芦醇对热应激诱导的山羊小肠上皮细胞炎性反应调节作用的研究[J].畜牧兽医学报,2020,51(8):1886-1894.ZHANG Y,XU Z J,HUANG X Y,et al.Regulatory effect of resveratrol on inflammatory response induced by heat stress in goat small intestinal epithelial cells[J].Acta Veterinaria et Zootechnica Sinica,2020,51(8):1886-1894.(in Chinese)
[15]	郭爱伟,刘莉莉,杨亚晋,等.植物多酚的生物活性及其在家禽生产中的应用[J].动物营养学报,2019,31(2):491-499.GUO A W,LIU L L,YANG Y J,et al.Research progress on bioactivity of plant polyphenols and application in poultry production[J].Chinese Journal of Animal Nutrition,2019,31(2):491-499.(in Chinese)
[16]	MENG Q W,SUN S S,BAI Y S,et al.Effects of dietary resveratrol supplementation in sows on antioxidative status,myofiber characteristic and meat quality of offspring[J].Meat Sci,2020,167:108176.
[17]	NASERIFAR M,HOSSEINZADEH H,ABNOUS K,et al.Oral delivery of folate-targeted resveratrol-loaded nanoparticles for inflammatory bowel disease therapy in rats[J].Life Sci,2020,262:118555.
[18]	SHAITO A,POSADINO A M,YOUNES N,et al.Potential adverse effects of resveratrol:a literature review[J].Int J Mol Sci,2020, 21(6):2084.
[19]	邓菊婴,胡睿智,肖定福,等.白藜芦醇缓解家禽热应激作用机制与应用研究进展[J].动物营养学报,2022,34(5):2773-2780.DENG J Y,HU R Z,XIAO D F,et al.Research progress on mechanism and application of resveratrol in alleviating heat stress in poultry[J].Chinese Journal of Animal Nutrition,2022,34(5):2773-2780.(in Chinese)
[20]	HWANG J W,YAO H W,CAITO S,et al.Redox regulation of SIRT1 in inflammation and cellular senescence[J].Free Radic Biol Med,2013,61:95-110.
[21]	ZHANG B,ZHAI M G,LI B Y,et al.Honokiol ameliorates myocardial ischemia/reperfusion injury in type 1 diabetic rats by reducing oxidative stress and apoptosis through activating the SIRT1-Nrf2 signaling pathway[J].Oxid Med Cell Longev,2018,2018:3159801.
[22]	HE S P,LI S,AROWOLO M A,et al.Effect of resveratrol on growth performance,rectal temperature and serum parameters of yellow-feather broilers under heat stress[J].Anim Sci J,2019,90(3):401-411.
[23]	CHEN X L,ZENG Z Y,HUANG Z Q,et al.Effects of dietary resveratrol supplementation on immunity,antioxidative capacity and intestinal barrier function in weaning piglets[J].Anim Biotechnol,2021,32(2):240-245.
[24]	FARAG M R,ALAGAWANY M.Physiological alterations of poultry to the high environmental temperature[J].J Therm Biol, 2018,76:101-106.
[25]	LI G M,LIU L P,YIN B,et al.Heat stress decreases egg production of laying hens by inducing apoptosis of follicular cells via activating the FasL/Fas and TNF-α systems[J].Poult Sci,2020,99(11):6084-6093.
[26]	卢倩,正旭城,梁雅茜,等.冷、热应激对水禽的影响及营养调控研究进展[J].饲料研究,2022,45(2):123-126.LU Q,ZHENG X C,LIANG Y Q,et al.Research progress on effect of cold and heat stress on waterfowl and nutritional regulation[J]. Feed Research,2022,45(2):123-126.(in Chinese)
[27]	GONZALEZ-RIVAS P A,CHAUHAN S S,HA M,et al.Effects of heat stress on animal physiology,metabolism,and meat quality:a review[J].Meat Sci,2020,162:108025.
[28]	刘迎生,焦洪超,林海,等.早期热应激对肉仔鸡后期生长发育的影响[J].畜牧兽医学报,2021,52(7):2052-2058.LIU Y S,JIAO H C,LIN H,et al.Effect of early heat stress on the growth and development of broilers[J].Acta Veterinaria et Zootechnica Sinica,2021,52(7):2052-2058.(in Chinese)
[29]	GOEL A.Heat stress management in poultry[J].J Anim Physiol Anim Nutr (Berl),2021,105(6):1136-1145.
[30]	DRIEDONKS N,XU J M,PETERS J L,et al.Multi-level interactions between heat shock factors,heat shock proteins,and the redox system regulate acclimation to heat[J].Front Plant Sci,2015,6:999.
[31]	ZININGA T,RAMATSUI L,SHONHAI A.Heat shock proteins as immunomodulants[J].Molecules,2018,23(11):2846.
[32]	ZHANG Q,ZHANG C,GE J,et al.Ameliorative effects of resveratrol against cadmium-induced nephrotoxicity via modulating nuclear xenobiotic receptor response and PINK1/Parkin-mediated mitophagy[J].Food Funct,2020,11(2):1856-1868.
[33]	KANG D,KIM S H,HAMASAKI N.Mitochondrial transcription factor A (TFAM):Roles in maintenance of mtDNA and cellular functions[J].Mitochondrion,2007,7(1-2):39-44.
[34]	WESTERHEIDE S D,ANCKAR J,STEVENS S M,et al.Stress-inducible regulation of heat shock factor 1 by the deacetylase SIRT1[J].Science,2009,323(5917):1063-1066.
[35]	ZHANG C,CHEN K K,ZHAO X H,et al.Protective effects of resveratrol against high ambient temperature-induced spleen dysplasia in broilers through modulating splenic redox status and apoptosis[J].J Sci Food Agric,2018,98(14):5409-5417.
[36]	储蓄,张军霞,王晶.动物氧化应激及其营养调控措施研究进展[J].畜牧兽医学报,2021,52(12):3346-3356.CHU X,ZHANG J X,WANG J.Research progress of animal oxidative stress and its nutritional regulation[J].Acta Veterinaria et Zootechnica Sinica,2021,52(12):3346-3356.(in Chinese)
[37]	BLOKHINA O,VIROLAINEN E,FAGERSTEDT K V.Antioxidants,oxidative damage and oxygen deprivation stress:a review[J]. Ann Bot, 2003,91(2):179-194.
[38]	HALLIWELL B.Reactive species and antioxidants.Redox biology is a fundamental theme of aerobic life[J].Plant Physiol,2006, 141(2):312-322.
[39]	豆梦莹,张才,李元晓,等.精氨酸对热应激处理的奶牛原代小肠上皮细胞增殖和凋亡的影响[J].畜牧兽医学报,2022, 53(2):493-504.DOU M Y,ZHANG C,LI Y X,et al.Effects of L-Arginine on proliferation and apoptosis of primary intestinal epithelial cells of dairy cows treated with heat stress in vitro[J].Acta Veterinaria et Zootechnica Sinica,2022,53(2):493-504.(in Chinese)
[40]	CHENG L,JIN Z X,ZHAO R,et al.Resveratrol attenuates inflammation and oxidative stress induced by myocardial ischemia-reperfusion injury:role of Nrf2/ARE pathway[J].Int J Clin Exp Med,2015,8(7):10420-10428.
[41]	XI H,FAN X,ZHANG Z,et al.Bax and Bcl-2 are involved in the apoptosis induced by local testicular heating in the boar testis[J].Reprod Domest Anim,2017,52(3):359-365.

Effects of Resveratrol on Anti-oxidation and Anti-apoptosis of Hepatocytes of Ducks on Exposure to Acute Heat Stress

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 41

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	DONG Shucan, MAO Shuaixiang, WU Cuiying, LI Yaokun, SUN Baoli, GUO Yongqing, DENG Ming, LIU Dewu, LIU Guangbin. The Effect of the Androgen Receptor Inhibitor Enzalutamide on Proliferation and Apoptosis of Goat Ovarian Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2022-2031.
[2]	WANG Jiying, YIN Ruiru, XIE Xing, WANG Haiyan, LIU Hudong, HU Hui, XIONG Qiyan, FENG Zhixin, SHAO Guoqing, YU Yanfei. Effects of LDH in Mesomycoplasma (Mycoplasma) hyopneumoniae on Apoptosis of Porcine Bronchial Epithelial Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2195-2205.
[3]	LI Qiuyun, TIAN Xinyuan, LIAO Wensheng, ZHANG Huanrong, REN Yupeng, YANG Falong, ZHU Jiangjiang, XIANG Hua. Effects of SOCS2 on Proliferation, Cycle and Apoptosis of Turbinate Bone Cells in Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2226-2240.
[4]	LAN Xinrui, ZHAO Baobao, ZHANG Bihan, LIN Xiaoyu, MA Huiming, WANG Yongsheng. Effects of β-sitosterol on Porcine Oocyte Maturation and Embryonic Development in Vitro [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1629-1637.
[5]	WANG Xiao, ZHANG Hao, LUAN Qingjiang, LI Hui, YANG Ding, WANG Tingyue, TIAN Jing, ZHAO Meng, CHEN Lu, TIAN Rugang. A Comprehensive Review of the Impact of Cold and Heat Stress on the Physiological Parameters and Gene Expression in Beef Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 894-904.
[6]	JIANG Lijun, ZONG Yunhe, LI Yunlei, CHEN Jilan, GENG Zhaoyu, SUN Yanyan, JIN Sihua. Research Progress of Antioxidant Application in Poultry Semen Storage [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 913-923.
[7]	HU Qiaoyan, ZHAI Xiangqin, LI Yidan, HAN Jiale, LEI Chuzhao, DANG Ruihua. Effects of bta-miR-101 on Proliferation, Apoptosis and Secretion of Bovine Testicular Sertoli Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1040-1051.
[8]	PIAN Huifang, DU Xubin, LI Yan, ZHANG Yuchen, HE Hui, YU Debing. Effects of Betaine on Performance, Egg Quality and Antioxidant Capacity of Late-phase Laying Hens [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1085-1094.
[9]	HUO Yuannan, QIU Meijia, ZHANG Jiaojiao, YANG Weirong, WANG Xianzhong. Arginine and Its Metabolites Attenuate Heat Stress-induced Apoptosis of Immature Boar Sertoli Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 587-597.
[10]	WANG Dong, LIU Kexin, HE Yanjun, DENG Shouxiang, LIU Yun, MA Weiming. Effects of Dietary Sodium Humate Supplementation on Liver Tissue Inflammation and Antioxidant Capacity of Salmonella Typhimurium-Infected Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 629-639.
[11]	QIU Wenyue, SU Yiman, YE Jiali, ZHANG Xinting, PANG Xiaoyue, WANG Rongmei, XIE Zimao, ZHANG Hui, TANG Zhaoxin, SU Rongsheng. Study on Asiatic Acid Alleviates LPS-induced Acute Kidney Injury by Regulating Apoptosis and Autophagy of Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 809-821.
[12]	XIAO Yimei, WANG Shengnan, XU Yuewen, HE Xiaolin, YIN Fuquan. Research on the Influence of Heat Stress on Male Reproduction [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 11-21.
[13]	CHEN Songbiao, LIU Feifei, SHANG Ke, YU Zuhua, HE Lei, WEI Ying, CHEN Jian, ZHANG Chunjie, CHENG Xiangchao, DING Ke. Molecular Mechanism of the “Battle” between Virus Infection and Host Antiviral Immunity-Apoptosis, Necroptosis and Pyroptosis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 59-70.
[14]	DUAN Xiangru, KANG Jia, YANG Ruochen, SHAN Xinyu, LI Taichun, ZHAO Wen, ZHANG Yingjie, LIU Yueqin. Effect of L-cysteine on Proliferation, Apoptosis and the Secretion of Steroid Hormone in Ovine Ovarian Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 179-191.
[15]	HAN Kunliang, LAN Wei, HU Xin, CUI Yadong, KONG Xiangfeng. Effects of Compound Chinese Herb Ultrafine Powder on Antioxidant Ability and Related Gene Expression in Laying Hens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3784-3792.