89株副猪嗜血杆菌临床分离株血清型、基因型鉴定与分析

doi:10.11843/j.issn.0366-6964.2020.11.019

Abstract

Abstract: In this study, we aimed to determine the tissue distribution of clinical isolates of Haemophilus parasuis (HPS) to explore the epidemic distribution and correlation of its serotypes and genotypes, and to provide scientific basis for the effective prevention and control of Glässer's disease. According to the 89 strains of HPS isolated from the clinic, the serotypes of HPS were identified by PCR, and the number of HPS strains in different parts of the isolates was counted. Sequence type (ST) identification analysis, site polymorphism analysis, BURST cluster analysis and UPGMA phylogenetic tree cluster analysis were performed by using the multilocus sequence typing method. Nine serotypes (1, 2, 4, 5, 7, 11, 12, 13, and 14) and undetermined serotypes (NT) were identified from 89 HPS clinical isolates. Serotypes 4,13,7 and 5 were the predominant serotypes, accounting for 28.09%, 22.47%, 13.48% and 10.11%, respectively, and sixty-four strains of HPS were isolated from the lung tissues. ST267, ST268, ST387 and ST365 were dominant genotypes, accounting for 26.97%, 21.35%, 8.99% and 5.62%, respectively, there were 3-13 alleles at each locus, and the polymorphisms ranged from 3(g3pd) to 71(6pgd). The BURST analysis showed that the 89 HPS were divided into 2 single ST types and 11 clonal groups (CC). The phylogenetic tree of UPGMA had 4 branches, the dominant ST type was found in 2, 3 and 4 branches, which was corresponded to the HPS isolates with virulent serotype. The epidemic serotypes and genotypes of HPS are diversified and have obvious genetic heterogeneity. At the same time, ST types and serotypes have some crossover and are related to HPS clinical pathogenicity.

Key words: Haemophilus parasuis, isolated strain, serotype, genotype

CLC Number:

S852.612

WANG Di, CHEN Zhang, XING Gang, HE Changsheng, LIU Xiaolu, WEI Jianzhong, SUN Pei, LIU Xuelan, LI Yu. Identification and Analysis of Serotype and Genotype of 89 Clinical Isolates of Haemophilus parasuis[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(11): 2802-2811.

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Identification and Analysis of Serotype and Genotype of 89 Clinical Isolates of Haemophilus parasuis

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