致脑膜炎猪链球菌2型的分子分型鉴定及其生物学特性

doi:10.11843/j.issn.0366-6964.2024.03.031

Abstract

Abstract: The study was design to understand the molecular typing, pathogenicity and biological characteristics of Streptococcus suis meningitidis type 2 from swine. In this study, a strain of Streptococcus suis PX0923 was isolated from the brain tissue of diseased pigs, and its Serotype and molecular typing were identified by electron microscopy, 16S rRNA gene sequencing, multiplex PCR, PCR-RFLP and multiple site sequence analysis (MLST), the pathogenicity and drug sensitivity of the isolated strain were analyzed by virulence gene, biofilm formation ability, drug resistance gene detection, mouse infection test, antibiotic and Chinese medicine susceptibility test. The results showed that the isolated strain PX0923 belongs to Gram-positive bacteria. Under electron microscopy, continuous arrangement of oval shaped mycelium can be seen in chains. In terms of 16S rRNA gene sequence analysis and phylogenetic tree construction, the isolated strain was identified as S. suis type 2. MLST (multilocus sequence typing) analysis confirmed that it is type ST7 and merged with type ST1. The isolated strain PX0923 carried seven virulence genes, epf, mrp, sly, gapdh, fbps, orf2 and sao, and showed α hemolytic activity. After artificial infection of PX0923, it was found that the infected mice presented meningitis and other symptoms similar to the natural diseases, such as bleeding in the lung, spleen and liver of the infected mice. The LD₅₀ of the isolated strain was 1.08×10⁹ CFU per mice, these findings suggest that PX0923 may cause meningitis in infected animals. PX0923 carrys resistant genes aadA1 and bla-TEM. PX0923 is resistant to 12 drugs such as Gentamicin, kanamycin and tetracycline, and sensitive to traditional Chinese medicine scorpion and panax notoginseng. In summary, Streptococcus suis PX0923 from pigs was isolated as serotype 2, and MLST was classified as ST7, and carries a variety of virulence genes and drug resistance genes. Artificial infection of mice can cause multiple tissue damage such as meningitis in mice. It is sensitive to various antibiotics and traditional Chinese medicine scorpion, Panax notoginseng. The research results provide a reference basis for the prevention and treatment of streptococci and vaccine research. The results of this study provide a molecular biological basis for the epidemic and source analysis of S. suis, and provide a reference for the prevention and control of S. suis Meningitidis type 2 infection

Key words: Streptococcus suis type 2, molecular subtyp, ST-7, serotype, resistance, traditional Chinese medicine

CLC Number:

S852.611

LI Pengxu, LI Shijing, SUN Jun, XIANG Wei, ZHAO Miaomiao, HOU Tianmu, LI Huaming, GUANG Min, CHEN Ruige, XU Mengran, WU Xiaomin, JIANG Hexiang, LEI Liancheng, ZHANG Fuxian. Molecular Subtyping and Identification of Streptococcus suis Meningitidis Type 2 and Its Biological Characteristics[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1192-1207.

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Molecular Subtyping and Identification of Streptococcus suis Meningitidis Type 2 and Its Biological Characteristics

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