我国主要流行的4种血清型禽腺病毒-I群感染SPF鸡的致病性比较研究

doi:10.11843/j.issn.0366-6964.2022.05.027

Abstract

Abstract: This study was conducted to investigate the pathogenicity of four serotypes of fowl adenovirus (FAdV-4, -8a, -8b and -11) that popular in China in chicken embryos and SPF chickens. Here, 12-day-old SPF chicken embryos, 10-day-old SPF chickens were infected with FAdV-4, -8a, -8b and -11 strains, respectively, the mortality of them were calculated, and body weight of embryo were measured, then gross lesions, histopathological examination and viral load in the tissues of chicken were tested. The results showed that embryo mortality was more than 45% for FAdV-8b and -11, and embryo weight loss was the greatest for FAdV-8a and smallest for FAdV-4. Chicken mortality was only present with FAdV-4 infections (53.3%) and no chicken was dead in other groups infected with FAdV-8a, -8b and -11. Besides lesions in the liver, hydropericardium, gizzard erosions, proventricular swelling and pancreatic necrosis were characteristic of chickens infected with FAdV-4, -8a, -8b and -11, respectively, and characterized by cell necrosis in parenchymal tissues accompanied by inflammatory cell infiltration. All immune organs in infected chickens possessed lesions. Compared with lesions caused by infection with FAdV-4, serious lesions were observed in chicken infected with FAdV-8a, -8b and -11. The results showed that viral loads in pancreas of chicken infected with FAdV-8b and -11 were higher than that infected with FAdV-4, and the viral loads in heart, liver and kidney of chicken infected with FAdV-4 at 5 days post infection were higher than those at 3 and 7 days post infection, and the viral load in the organs were consistent with the degree of damage in all the chickens infected, except the chicken infected with FAdV-8a. Here, we describe for the first time that the pathogenicity of these four serotypes FAdVs in embryos do not represent their pathogenicity to chickens. More attention also should be paid to FAdV-8a, -8b and -11 infections due to their severe lesions in the embryos that of the result of vertical transmission, and in the organs of the digestive and immune system that does not result in direct mortality but retard to growth and prone to secondary infection with other pathogens.

Key words: fowl adenovirus, pathogenicity, serotype, SPF chicken, embryos

CLC Number:

WANG Jianlin, JIANG Zhiyao, LUAN Qingdong, YIN Yanbo. Comparative Pathological Analysis of SPF Chicken Host Infected with Serotype 4, 8a, 8b and 11 Fowl Adenovirus Popular in China[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1598-1607.

References 23

[1]	ABSALÓN A E, MORALES-GARZÓN A, VERA-HERNÁNDEZ P F, et al. Complete genome sequence of a non-pathogenic strain of Fowl Adenovirus serotype 11:minimal genomic differences between pathogenic and non-pathogenic viruses[J]. Virology, 2017, 501:63-69.
[2]	MASE M, NAKAMURA K. Phylogenetic analysis of fowl adenoviruses isolated from chickens with gizzard erosion in Japan[J]. J Vet Med Sci, 2014, 76(11):1535-1538.
[3]	OKUDA Y, ONO M, SHIBATA I, et al. Pathogenicity of serotype 8 fowl adenovirus isolated from gizzard erosions of slaughtered broiler chickens[J]. J Vet Med Sci, 2004, 66(12):1561-1566.
[4]	ONO M, OKUDA Y, YAZAWA S, et al. Outbreaks of adenoviral gizzard erosion in slaughtered broiler chickens in Japan[J]. Vet Rec, 2003, 153(25):775-779.
[5]	SCHACHNER A, MATOS M, GRAFL B, et al. Fowl adenovirus-induced diseases and strategies for their control-a review on the current global situation[J]. Avian Pathol, 2018, 47(2):111-126.
[6]	LI C J, LI H Y, WANG D D, et al. Characterization of fowl adenoviruses isolated between 2007 and 2014 in China[J]. Vet Microbiol, 2016, 197:62-67.
[7]	WANG J L, WANG S C, ZOU K Y, et al. Variant serotypes of fowl adenovirus isolated from commercial poultry between 2007 and 2017 in some regions of China[J]. Avian Dis, 2018, 62(2):171-176.
[8]	LIU Y K, WAN W Y, GAO D S, et al. Genetic characterization of novel fowl aviadenovirus 4 isolates from outbreaks of hepatitis-hydropericardium syndrome in broiler chickens in China[J]. Emerg Microbes Infec, 2016, 5(11):e117.
[9]	NIU Y J, SUN Q Q, ZHANG G H, et al. Pathogenicity and immunosuppressive potential of fowl adenovirus in specific pathogen free chickens[J]. Poult Sci, 2017, 96(11):3885-3892.
[10]	STEER P A, SANDY J R, O'ROURKE D, et al. Chronological analysis of gross and histological lesions induced by field strains of fowl adenovirus serotypes 1, 8b and 11 in one-day-old chickens[J]. Avian Pathol, 2015, 44(2):106-113.
[11]	MATOS M, GRAFL B, LIEBHART D, et al. Selected clinical chemistry analytes correlate with the pathogenesis of inclusion body hepatitis experimentally induced by fowl aviadenoviruses[J]. Avian Pathol, 2016, 45(5):520-529.
[12]	MENDELSON C, NOTHELFER H B, MONREAL G. Identification and characterization of an avian adenovirus isolated from a 'spiking mortality syndrome' field outbreak in broilers on the Delmarva Peninsula, USA[J]. Avian Pathol, 1995, 24(4):693-706.
[13]	ALEMNESH W, HAIR-BEJO M, AINI I, et al. Pathogenicity of fowl adenovirus in specific pathogen free chicken embryos[J]. J Comp Pathol, 2012, 146(2-3):223-229.
[14]	MAZAHERI A, PRUSAS C, VOß M, et al. Some strains of serotype 4 fowl adenoviruses cause inclusion body hepatitis and hydropericardium syndrome in chickens[J]. Avian Pathol, 1998, 27(3):269-276.
[15]	REED L J, MUENCH H. A simple method of estimating fifty per cent endpoints[J]. Am J Epidemiol, 1938, 27(3):493-497.
[16]	MEULEMANS G, BOSCHMANS M, VAN DEN BERG T P, et al. Polymerase chain reaction combined with restriction enzyme analysis for detection and differentiation of fowl adenoviruses[J]. Avian Pathol, 2001, 30(6):655-660.
[17]	ZHAO J, RUAN S F, GUO Y, et al. Serological and phylogenetic analysis indicating prevalence of fowl adenovirus in China[J]. Vet Rec, 2018, 182(13):381.
[18]	HUANG Q H, MA X X, HUANG X Y, et al. Pathogenicity and complete genome sequence of a fowl adenovirus serotype 8b isolate from China[J]. Poult Sci, 2019, 98(2):573-580.
[19]	RADWAN M M, EL-DEEB A H, MOUSA M R, et al. First report of fowl adenovirus 8a from commercial broiler chickens in Egypt:molecular characterization and pathogenicity[J]. Poult Sci, 2019, 98(1):97-104.
[20]	ZHANG H W, JIN W J, DING K, et al. Genetic characterization of fowl adenovirus strains isolated from poultry in China[J]. Avian Dis, 2017, 61(3):341-346.
[21]	ZHANG T, JIN Q Y, DING P Y, et al. Molecular epidemiology of hydropericardium syndrome outbreak-associated serotype 4 fowl adenovirus isolates in central China[J]. Virol J, 2016, 13(1):188.
[22]	LI P H, ZHENG P P, ZHANG T F, et al. Fowl adenovirus serotype 4:epidemiology, pathogenesis, diagnostic detection, and vaccine strategies[J]. Poult Sci, 2017, 96(8):2630-2640.
[23]	NAKAMURA K, TANAKA H, MASE M, et al. Pancreatic necrosis and ventricular erosion in adenovirus-associated hydropericardium syndrome of broilers[J]. Vet Pathol, 2002, 39(3):403-406.

Comparative Pathological Analysis of SPF Chicken Host Infected with Serotype 4, 8a, 8b and 11 Fowl Adenovirus Popular in China

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 23

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Chenjian, LI Yinxia, DING Qiang, LIU Weijia, WANG Huili, HE Nan, WU Jiashun, CAO Shaoxian. Efficient Preparation of CRISPR/Cas9-mediated Goat SOCS2 Gene Edited Embryos [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 129-141.
[2]	CHEN Yuetong, LIU Xiaohan, WANG Zhiyang, ZHAO Yuxin, ZHOU Tiezhong, HU Zengjin, ZHU Yue, WANG Shaohui, TIAN Mingxing, DING Siyu, QI Jingjing, YU Shengqing. Isolation, Identification, Pathogenicity and Drug Susceptibility of Mycoplasma gallisepticum from Dead Chicken Embryos in Large-scale Chicken Farms in Guangdong Province [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 290-299.
[3]	YANG Heng, LI Zhanhong, SONG Zi'ang, GAO Lin, LI Zhuoran, LIAO Defang, XIAO Lei, LI Huachun. Development and Application of Serotyping Real-time Quantitative RT-PCR Methods for Palyam Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 395-400.
[4]	ZHANG Yi, WANG Chenyan, YANG Xiao, SHAO Guoqing, HOU Bo. Phylogenetic Analysis and Pathogenicity of an Avian Infectious Laryngotracheitis Virus Strain WF03 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3435-3444.
[5]	WANG Jiali, ZHOU Ning, CHEN Xi, YUE Hua, TANG Cheng. Isolation, Identification and Pathogenicity of Canine Adenovirus Type 2 Strain with Natural Deletion in E3 Gene [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2982-2990.
[6]	CAO Xiuyun, LIU Jiwen, TANG Zhihui, ZHENG Ziyi, YAN Liping, SONG Suquan. Isolation, Identification and Pathogenicity Analysis of a Duck Adenovirus Type 3 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2050-2061.
[7]	YUAN Sheng, LI Anqi, Lü Wenke, YANG Lulu, ZHOU Feng, HUANG Liangzong, BAI Aiquan, WEN Feng, HUANG Shujian, GUO Jinyue. Analysis of Variation in Major Virulence-related Genes of a Strain of Pseudorabies Virus and Its Pathogenicity to Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2195-2199.
[8]	KU Xugang, YU Xuexiang, SUN Qi, LI Panpan, ZHANG Mengjia, LUO Rui, QIAN Ping, HE Qigai. Isolation and Pathogenicity Analysis of Porcine Circovirus Type 3 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1568-1578.
[9]	NI Zheng, YE Weicheng, CHEN Liu, YUN Tao, HUA Jionggang, ZHU Yinchu, ZHANG Cun. Genetic Variation and Pathogenicity of a Pseudorabies Virus Variant Strain in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1766-1770.
[10]	JIANG Zenghai, TENG Lin, HE Anwen, LIU Yanyan, YUE Min, HE Qigai. Genomic Analysis of Salmonella Typhimurium Isolates and Salmonella Serotype 4, [5], 12: i:- Isolates from Pig-borne Food Chain [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1199-1209.
[11]	MENG Lingzhai, CHEN Chunli, YU Mengmeng, WANG Zhanxin, WANG Suyan, LIU Peng, HE Tana, GUO Ru, CHEN Yuntong, LIU Changjun, QI Xiaole, WU Zhiqiang, GAO Yulong. Pathogenicity of Avian Metapneumovirus Subtype B on Yellow Feather Broilers and Evaluation on Immune Effect of Inactivated Vaccine [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5154-5161.
[12]	CHI Lili, WANG Junna, ZHANG Yuming, LIU Jian, CHEN Zhiyuan, LI Shufan, YIN Yanbo, XU Shouzhen. Prokaryotic Expression and Immunogenicity Analysis of Truncated Fusion Protein of FAdV-4 Fiber2 and FAdV-8b Fiber [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5162-5170.
[13]	YANG Menglin, ZHENG Shiqi, PENG Kai, WANG Wei, HUANG Yanhua, PENG Jie. Isolation and Identification of Pigeon-derived Salmonella Typhimurium and Pathogenic Analysis [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4880-4888.
[14]	LI Shun, LI Guizhen, YUAN Yaoxian, LI Kangjian, MA Chunquan, LI Shoujun, HUANG Liangzong, MA Jun. Effect of the Length of PA-X Gene on Replication and Pathogenicity of H3N2 Canine Influenza Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 272-280.
[15]	CHENG Baoyu, LI Zihe, CUI Yanlei, LI Jiahui, YANG Xinwei, YU Yongle, YANG Haiyan, SHAN Hu, ZHANG Chuanmei. Genetic Evolution of Feline Parvovirus and Pathogenicity of an Isolated Strains [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3121-3131.