牛凸隆病毒HE基因分子特征分析

doi:10.11843/j.issn.0366-6964.2021.07.029

Abstract

Abstract: The purpose of this study was to characterize the molecular characteristics of the HE genes of bovine torovirus (BToV). An RT-PCR assay was used to detect BToV from 94 diarrheic fecal samples collected in Liaoning, Henan, and Sichuan provinces and full-length hemagglutinin-esterase (HE) genes were further amplified from the positive samples. Approximate 10.64% (10/94) samples were detected as BToV positive, and 15 full-length HE genes were sequenced from 10 positive samples, including 9 genotype Ⅱ strains and 6 genotype Ⅲ strains. Among the 10 positive samples, 5 samples were co-infected with genotype Ⅱ and Ⅲ strains, 1 sample was infected with genotype Ⅲ only and 4 samples were infected with genotype Ⅱ strains only. Compared with other available genotype Ⅲ BToV HE genes in the GenBank database, 4 unique amino acid variations were found in the HE gene, including 2 variations in the lectin domain which may affect HE receptor binding. Recombination analysis showed that BToV genotype Ⅲ strains may be derived from the recombination of genotype Ⅱand genotype Ⅰstrains in the HE gene. This study is the first confirmation of genotype Ⅲ BToV in China and the co-infection of genotype Ⅱ and Ⅲ strains. These findings contributed to the diagnosis and control of bovine diarrhea in China and enhance the current understanding of the genetic evolution of BToV.

Key words: bovine torovirus, HE gene, genotype Ⅲ, recombination

CLC Number:

S852.659.6

ZHAO Long, TANG Cheng, YUE Hua. Molecular Characteristics Analysis of HE Genes of Bovine Torovirus[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(7): 2065-2072.

References 31

[1]	HU Z M, YANG Y L, XU L D, et al. Porcine Torovirus (PToV)-a brief review of etiology, diagnostic assays and current epidemiology[J]. Front Vet Sci, 2019, 6:120.
[2]	HOET A E, SMILEY J, THOMAS C, et al. Association of enteric shedding of bovine torovirus (Breda virus) and other enteropathogens with diarrhea in veal calves[J]. Am J Vet Res, 2003, 64(4):485-490.
[3]	AITA T, KUWABARA M, MURAYAMA K, et al. Characterization of epidemic diarrhea outbreaks associated with bovine torovirus in adult cows[J]. Arch Virol, 2012, 157(3):423-431.
[4]	ITO T, OKADA N, OKAWA M, et al. Detection and characterization of bovine torovirus from the respiratory tract in Japanese cattle[J]. Vet Microbiol, 2009, 136(3-4):366-371.
[5]	HOET A E, CHO K O, CHANG K O, et al. Enteric and nasal shedding of bovine torovirus (Breda virus) in feedlot cattle[J]. Am J Vet Res, 2002, 63(3):342-348.
[6]	WOODE G N, REED D E, RUNNELS P L, et al. Studies with an unclassified virus isolated from diarrheic calves[J]. Vet Microbiol, 1982, 7(3):221-240.
[7]	NOGUEIRA J S, ASANO K M, DE SOUZA S P, et al. First detection and molecular diversity of Brazilian bovine torovirus (BToV) strains from young and adult cattle[J]. Res Vet Sci, 2013, 95(2):799-801.
[8]	LI H, ZHANG B, YUE H, et al. First detection and genomic characteristics of bovine torovirus in dairy calves in China[J]. Arch Virol, 2020, 165(7):1577-1583.
[9]	GÜLAÇTI I, IŞIDAN H, SÖZDUTMAZ I. Detection of bovine torovirus in fecal specimens from calves with diarrhea in Turkey[J]. Arch Virol, 2014, 159(7):1623-1627.
[10]	LOJKIĆ I, KREŠIĆN, ŠIMIĆ I, et al. Detection and molecular characterisation of bovine corona and toroviruses from croatian cattle[J]. BMC Vet Res, 2015, 11(1):202.
[11]	DUCKMANTON L, CARMAN S, NAGY É, et al. Detection of bovine torovirus in fecal specimens of calves with diarrhea from ontario farms[J]. J Clin Microbiol, 1998, 36(5):1266-1270.
[12]	SHI Z H, WANG W J, CHEN C X, et al. First report and genetic characterization of bovine torovirus in diarrhoeic calves in China[J]. BMC Vet Res, 2020, 16(1):272.
[13]	PÉREZ E, KUMMELING A, JANSSEN M M H, et al. Infectious agents associated with diarrhoea of calves in the canton of Tilarán, Costa Rica[J]. Prev Vet Med, 1998, 33(1-4):195-205.
[14]	HASCHEK B, KLEIN D, BENETKA V, et al. Detection of bovine torovirus in neonatal calf diarrhoea in lower austria and styria (Austria)[J]. J Vet Med B Infect Dis Vet Public Health, 2006, 53(4):160-165.
[15]	DRAKER R, ROPER R L, PETRIC M, et al. The complete sequence of the bovine torovirus genome[J]. Virus Res, 2006, 115(1):56-68.
[16]	LANGEREIS M A, ZENG Q H, GERWIG G J, et al. Structural basis for ligand and substrate recognition by torovirus hemagglutinin esterases[J]. Proc Nat Acad Sci U S A, 2009, 106(37):15897-15902.
[17]	DE GROOT R J. Structure, function and evolution of the hemagglutinin-esterase proteins of corona- and toroviruses[J]. Glycoconj J, 2006, 23(1-2):59-72.
[18]	HOET A E, SAIF L J. Bovine torovirus (Breda virus) revisited[J]. Anim Health Res Rev, 2004, 5(2):157-171.
[19]	CORNELISSEN L A, WIERDA C M, VAN DER MEER F, et al. Hemagglutinin-esterase, a novel structural protein of torovirus[J]. J Virol, 1997, 71(7):5277-5286.
[20]	HERRLER G, KLENK H D. Structure and function of the Hef glycoprotein of influenza C virus[J]. Adv Virus Res, 1991, 40:213-234.
[21]	RIMSTAD E, MARKUSSEN T. Infectious salmon anaemia virus-molecular biology and pathogenesis of the infection[J]. J Appl Microbiol, 2020, 129(1):85-97.
[22]	ZENG Q H, LANGEREIS M A, VAN VLIET A L W, et al. Structure of coronavirus hemagglutinin-esterase offers insight into corona and influenza virus evolution[J]. Proc Nat Acad Sci U S A, 2008, 105(26):9065-9069.
[23]	LANGEREIS M A, ZENG Q H, HEESTERS B, et al. The murine coronavirus hemagglutinin-esterase receptor-binding site:a major shift in ligand specificity through modest changes in architecture[J]. PLOS Pathog, 2012, 8(1):e1002492.
[24]	DEREGT D, BABIUK L A. Monoclonal antibodies to bovine coronavirus:characteristics and topographical mapping of neutralizing epitopes on the E2 and E3 glycoproteins[J]. Virology, 1987, 161(2):410-420.
[25]	MATSUZAKI Y, SHIMOTAI Y, KADOWAKI Y, et al. Antigenic changes among the predominantly circulating C/Sao Paulo lineage strains of influenza C virus in Yamagata, Japan, between 2015 and 2018[J]. Infect Genet Evol, 2020, 81:104269.
[26]	SMITS S L, LAVAZZA A, MATIZ K, et al. Phylogenetic and evolutionary relationships among torovirus field variants:evidence for multiple intertypic recombination events[J]. J Virol, 2003, 77(17):9567-9577.
[27]	ITO M, TSUCHIAKA S, NAOI Y, et al. Whole genome analysis of Japanese bovine toroviruses reveals natural recombination between porcine and bovine toroviruses[J]. Infect Genet Evol, 2016, 38:90-95.
[28]	刘华南, 曹伟军, 杨帆, 等. 凸隆病毒研究进展[J]. 畜牧兽医学报, 2013, 44(8):1173-1181.LIU H N, CAO W J, YANG F, et al. Research progress of torovirus[J]. Acta Veterinaria et Zootechnica Sinica, 2013, 44(8):1173-1181. (in Chinese)
[29]	赵龙, 汤承, 岳华. 牦牛源牛凸隆病毒的检测及其基因组特征[J]. 畜牧兽医学报, 2021, 52(2):469-477.ZHAO L, TANG C, YUE H. Detection and genomic characteristics of bovine torovirus in yak[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(2):469-477. (in Chinese)
[30]	DUCKMANTON L M, TELLIER R, LIU P, et al. Bovine torovirus:sequencing of the structural genes and expression of the nucleocapsid protein of Breda virus[J]. Virus Res, 1998, 58(1-2):83-96.
[31]	SMITS S L, GERWIG G J, VAN VLIET A L W, et al. Nidovirus sialate-O-acetylesterases:Evolution and substrate specificity of coronaviral and toroviral receptor-destroying enzymes[J]. J Biol Chem, 2005, 280(8):6933-6941.

Molecular Characteristics Analysis of HE Genes of Bovine Torovirus

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 31

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	FAN Yandi, YANG Danjiao, YE Zhongming, ZHANG Min, LAN Lan, WANG Jinghao, ZHOU Han, KANG Runmin, YU Jifeng, ZHANG Zhidong, LI Yanmin, ZHOU Long. Metagenomic Analysis of Viruses in Clinical Samples of Respiratory Diseases in Tibetan Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3836-3847.
[2]	FAN Wensheng, LIU Sijia, QIU Shenben, HUANG Aifang, WANG Yan, LIU Minfang, MEI Minmin, CHEN Xinliang, WEI Ping, MO Meilan. Genetic Evolution and Spatio-temporal Transmission Analysis of the Chinese Infectious Bronchitis Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2543-2554.
[3]	LIU Jiankui, XU Ye, LIU Chen, YU Hui, YANG Yuan, HE Le, LI Jiarui, DAN Huijuan, DAI Ailing, YANG Xiaoyan, WEI Chunhua. Genomic Characteristics of Porcine Reproductive and Respiratory Syndrome Virus in Fujian Province from 2017 to 2021 based on Whole Genome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1579-1589.
[4]	CAO Li, CHENG Runan, WU Zhouhui, WANG Jiawei, WANG Yu, ZHANG Yonghong, WU Qingmin, WANG Zhen. Construction and Biological Characteristics of sapC Gene Deletion Strain of Salmonella Typhimurium [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2282-2289.
[5]	LINGHU Yuanfeng, PAN Yong, YANG Yang, DUAN Shiyu, ZHANG Jiali, ZHANG Baotai, YANG Qi. Preliminary Exploration of Binding Sites of Salmonella Typhimurium Chaperone Protein Hfq on Small RNA GcvB [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4110-4115.
[6]	ZHAO Long, TANG Cheng, YUE Hua. Detection and Genomic Characteristics of Bovine Torovirus in Yak [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(2): 469-477.
[7]	ZHAO Long, LI Hao, TANG Cheng, YUE Hua. Research Progress of Bovine Torovirus [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2753-2761.
[8]	WANG Lixuan, ZHOU Qun, FU Nengsheng, CAO Hui, HE Xinyi, FANG Pengfei, HU Chengzhe, ZHANG Bin. Detection and Genetic Evolution of Teschovirus of Tibetan Pig Origin in Sichuan [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(1): 185-194.
[9]	GAO Shandian, WANG Jinming, TIAN Zhancheng, DU Junzheng, ZHANG Zhonghui, WANG Jiandong, KANG Xiaodong, LI Youquan, GUAN Guiquan, LIU Guangyuan, LUO Jianxun, YIN Hong. Genomic Characteristics of a Bovine Viral Diarrhea Virus Isolate [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(8): 1913-1922.
[10]	GUO Zhenhua, RUAN Haiyu, GENG Rui, CHEN Xinxin, QIAO Songlin, DENG Ruiguang, ZHANG Gaiping. Genetic Characteristics of a Recombinant Porcine Reproductive and Respiratory Syndrome Virus between NADC30-like and JXA1-like [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(7): 1677-1687.
[11]	ZHOU Long, KANG Runmin, ZHANG Yi, XIE Bo, YU Jifeng, LI Chun, ZHANG Bin, TANG Cheng, WANG Hongning. Whole Genome Analysis of Eight Porcine Reproductive and Respiratory Syndrome Viruses Isolated in Sichuan and Guizhou Provinces [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1382-1390.
[12]	TAN Xiangmei, WU Xia, CHEN Pengfei, ZHAO Xiongwei, ZHOU Shuting, YU Lingxue, TONG Wu, GAO Fei, JIANG Yifeng, YU Hai, TONG Guangzhi, ZHOU Yanjun. Genome Characterization of a Recombinant Porcine Reproductive and Respiratory Syndrome Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(12): 3181-3186.
[13]	ZHU Kemeng, JIN Jin, WANG Ning, XIN Jiuqing, LIU Henggui. Establishment of E. coli Model for the Research of Lipoate-Protein Ligases [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1073-1081.
[14]	HE Qifu, TANG Cheng, GUO Zijing, TAN Shuo, ZHANG Bin, YUE Hua. Amplification, Sequence Analysis of Bovine Coronavirus Genes and Isolation of the Viruses from Yak [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 343-353.
[15]	ZHOU Zhong-wen, LIN Gui-fang, WANG Hao-hao, SU Shuai, CUI Zhi-zhong, SUN Shu-hong. Biocharacteristics of Recombinant Marek's Disease Virus Expressing the env Gene of Reticuloendotheliosis Virus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1482-1491.