牦牛源正呼肠孤病毒2型的检测和分离鉴定

doi:10.11843/j.issn.0366-6964.2021.08.030

Abstract

Abstract: This study aimed to investigate the infection of mammalian orthoreovirus (MRV) in Northwest Sichuan yaks and isolate MRV from positive samples. 72 fecal samples of diarrhea yaks from 15 pastures in northwest Sichuan and 15 serum samples of diarrhea yaks from 5 pastures were collected for detecting MRV by PT-PCR, and the positive samples were further serotyped by PCR assay. The results showed that the detection rates of MRV in diarrhea samples and serum samples were 20.83% (15/72) and 40% (6/15), respectively; and MRV2 was 60% (9/15) in MRV-positive diarrhea samples and 83.33% (5/6) in MRV-positive serum samples, respectively; other serotypes were not detected. One strain of MRV2 (TCID₅₀ is 4×10^-8.56·mL^-1) was isolated from a fecal sample, and its complete genome was successfully sequenced with 23 587 bp in length. In phylogenetic analysis of MRV genomes, the strain yak/AB14/2018 is closely related to China swine strains. Compared with all S1 sequences availble in GenBank, this strain shared 4 amino acids variations. In conclusion, MRV was detected from yaks, and one MRV2 strain was successfully isolated, contributing to further research the biological characteristics of MRV2.

Key words: yak, mammalian orthoreovirus, detection, serotype 2, isolation

CLC Number:

S852.659.4

LÜ Ting, CHEN Hongyin, TANG Cheng, YUE Hua. Detection, Isolation and Identification of Mammalian Orthoreovirus Serotype 2 in Yaks[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2361-2368.

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Detection, Isolation and Identification of Mammalian Orthoreovirus Serotype 2 in Yaks

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