奶牛源金黄色葡萄球菌新疆流行株的耐药特性、毒力基因及分子分型

doi:10.11843/j.issn.0366-6964.2018.01.021

Abstract

Abstract:

In order to know the antimicrobial resistance, virulence genes and the epidemiological characteristics of Staphylococcus aureus (SA) isolated from cows with mastitis and endometritis. The drug resistance profile for 155 clinical S. aureus isolates were determined using disk diffusion method, and methicillin-resistant Staphylococcus aureus (MRSA) strains were also identified. Antimicrobial genes, virulence genes, SCCmec genotyping and MLST genotyping were detected and analyzed by using PCR method. The results showed that a total of 22 MRSA strains have been detected among 155 SA isolates, with the detection rate of 14.2%. The antimicrobial resistant rates of most MRSA were significantly higher than that of the MSSA strains; and the detection rates of different virulence genes were different between MRSA and MSSA. The SCCmecⅠwas identified as a predominant genotype of SCCmec in Xinjiang region. Fourteen ST genotypes were detected, namely ST188, ST584, ST9, ST805, ST2373, ST968, ST2139, ST1, ST2700, ST903, ST2454, ST2990, ST63 and STX, respectively. ST1 and ST9 had higher detection rates than others. ST9 was higher among MSSA strains and ST1 was higher among MRSA strains. This study showed that SA strains were of multiple resistance with diverse distribution of virulence genes. While ST9 was the predominant genotype among MSSA strains and ST1-SCCmecⅠwas the predominant genotypes among MRSA strains in Xinjiang region.

CLC Number:

S852.611

MENG Dan, MENG Qing-ling, QIAO Jun, CAI Kuo-jun, WANG Deng-feng, MA Shuai, WU Ye-hui, CAI Xue-peng. Study on Resistance Characteristics, Virulence Genes and Molecular Classification of Staphylococcus aureus Isolated from Cows in Xinjiang[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(1): 181-194.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2018.01.021

https://www.xmsyxb.com/EN/Y2018/V49/I1/181

References

[1] GENG W J, YANG Y H, WU D J, et al. Molecular characteristics of community-acquired, methicillin-resistant Staphylococcus aureus isolated from Chinese children[J]. FEMS Immunol Med Microbiol, 2010, 58(3):356-362.
[2] ASⅡMWE B B, BALDAN R, TROVATO A, et al. Prevalence and molecular characteristics of Staphylococcus aureus, including methicillin resistant strains, isolated from bulk can milk and raw milk products in pastoral communities of South-West Uganda[J]. BMC Infect Dis, 2017, 17:422.
[3] THAPALIYA D, DALMAN M, KADARIYA J, et al. Characterization of Staphylococcus aureus in goose feces from state parks in Northeast Ohio[J]. EcoHealth, 2017, 14(2):303-309.
[4] 潘引君, 谢玲丽, 任志华, 等. 医院与社区获得性金黄色葡萄球菌耐药性分析[J]. 职业与健康, 2014, 30(7):898-900.
PAN Y J, XIE L L, REN Z H, et al. Analysis on drug resistance of hospital-acquired and community-acquired Staphylococcus aureus[J]. Occupation and Health, 2014, 30(7):898-900. (in Chinese)
[5] JENSEN S O, LYON B R. Genetics of antimicrobial resistance in Staphylococcus aureus[J]. Future Microbiol, 2009, 4(5):565-582.
[6] SUN W J, CHEN H B, LIU Y D, et al. Prevalence and characterization of heterogeneous vancomycin-intermediate Staphylococcus aureus isolates from 14 cities in China[J]. Antimicrob Agents Chemother, 2009, 53(9):3642-3649.
[7] 杨峰, 王旭荣, 李新圃, 等. 牛源金黄色葡萄球菌耐药性与相关耐药基因和菌株毒力基因的相关性研究[J]. 中国兽医科学, 2016, 46(2):247-252.
YANG F, WANG X R, LI X P, et al. Study on relationship between resistance and resistance-and virulence-related genes in Staphylococcus aureus from bovine mastitis cases[J]. Chinese Veterinary Science, 2016, 46(2):247-252. (in Chinese)
[8] MOMTAZ H, HAFEZI L. Meticillin-resistant Staphylococcus aureus isolated from Iranian hospitals:virulence factors and antibiotic resistance properties[J]. Bosn J Basic Med Sci, 2014, 14(4):219-226.
[9] 张蓉映, 朱健铭, 翁幸鐾. 耐甲氧西林金黄色葡萄球菌毒力与耐药基因分型研究[J]. 中华医院感染学杂志, 2014, 24(14):3384-3385, 3388.
ZHANG R Y, ZHU J M, WENG X B. Genotyping method depending on virulence factors and drug-resistant genes in methicillin-resistant Staphylococcus aureus[J]. Chinese Journal of Nosocomiology, 2014, 24(14):3384-3385, 3388. (in Chinese)
[10] MALACHOWA N, DELEO F R. Mobile genetic elements of Staphylococcus aureus[J]. Cell Mol Life Sci, 2010, 67(18):3057-3071.
[11] MAGRO G, BIFFANI S, MINOZZI G, et al. Virulence genes of S. aureus from dairy cow mastitis and contagiousness risk[J]. Toxins, 2017, 9(6):195.
[12] UDO E E, AL-SWEIH N, DHAR R, et al. Surveillance of antibacterial resistance in Staphylococcus aureus isolated in Kuwaiti hospitals[J]. Med Princ Pract, 2008, 17(1):71-75.
[13] KEHRENBERG C, CUNY C, STROMMENGER B, et al. Methicillin-resistant and-susceptible Staphylococcus aureus strains of clonal lineages ST398 and ST9 from swine carry the multidrug resistance gene cfr[J]. Antimicrob Agents Chemother, 2009, 53(2):779-781.
[14] 郝维聪. 奶牛隐性乳房炎的检测及预防[J]. 湖北畜牧兽医, 2016, 37(5):25-26.
HAO W C. Detection and prevention of mastitis of dairy cow[J]. Hubei Journal of Animal and Veterinary Sciences, 2016, 37(5):25-26. (in Chinese)
[15] 秦佳晨, 曹桂彬, 王新庄, 等. 牛子宫内膜炎临床诊断及采样方法的研究[C]//第五届中国牛业发展大会论文集. 滨州:中国畜牧业协会, 2010:6.
QIN J C, CAO G B, WANG X Z, et al. Clinical diagnosis and sampling method of endometritis in cattle[C]//National Cattle Industry Association. Binzhou:China Animal Agriculture Association, 2010:6. (in Chinese)
[16] 张晓平. 金黄色葡萄球菌耐药机制与分子流行病学研究[D]. 扬州:扬州大学, 2012.
ZHANG X P. Antimicrobial resistance and molecular epidemiology of Staphylococcus aureus[D]. Yangzhou:Yangzhou University, 2012. (in Chinese)
[17] DORMANESH B, SIROOSBAKHAT S, KHODAVERDI D E, et al. Methicillin-resistant Staphylococcus aureus isolated from various types of hospital infections in pediatrics:panton-valentine leukocidin, staphylococcal chromosomal cassette mec SCCmec phenotypes and antibiotic resistance properties[J]. Jundishapur J Microbiol, 2015, 8(11):e11341.
[18] STEPHAN R, ANNEMVLLER C, HASSAN A A, et al. Characterization of enterotoxigenic Staphylococcus aureus strains isolated from bovine mastitis in north-east Switzerland[J]. Vet Microbiol, 2001, 78(4):373-382.
[19] KHEMIRI M, AKROUT ALHUSAIN A, ABBASSI M S, et al. Clonal spread of methicillin-resistant Staphylococcus aureus-t6065-CC5-SCCmecV-agrⅡ in a Libyan hospital[J]. J Glob Antimicrob Resist, 2017, 10:101-105.
[20] ENRIGHT M C, DAY N P J, DAVIES C E, et al. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus[J]. J Clin Microbiol, 2000, 38(3):1008-1015.
[21] GOSTEV V, KRUGLOV A, KALINOGORSKAYA O, et al. Molecular epidemiology and antibiotic resistance of methicillin-resistant Staphylococcus aureus circulating in the Russian federation[J]. Infect Genet Evol, 2017, 53:189-194.
[22] OU Q, ZHOU J, LIN D, et al. A large meta-analysis of the global prevalence rates of S. aureus and MRSA contamination of milk[J]. Crit Rev Food Sci Nutr, 2017, 7:1-16.
[23] CONCEIÇÃO T, DE LENCASTRE H, AIRES-DE-SOUSA M. Healthy bovines as reservoirs of major pathogenic lineages of Staphylococcus aureus in portugal[J]. Microb Drug Resist, 2017, 23(7):845-851.
[24] XIAO M, WANG H, ZHAO Y, et al. National surveillance of methicillin-resistant Staphylococcus aureus in China highlights a still-evolving epidemiology with 15 novel emerging multilocus sequence types[J]. J Clin Microbiol, 2013, 51(11):3638-3644.
[25] COLLINS J, RUDKIN J, RECKER M, et al. Offsetting virulence and antibiotic resistance costs by MRSA[J]. ISME J, 2010, 4(4):577-584.
[26] 童俊, 占志平. 金黄色葡萄球菌毒力基因检测及分子分型研究[J]. 中华微生物学和免疫学杂志, 2015, 35(1):46-50.
TONG J, ZHAN Z P. Study of the virulence genes and molecular characteristics of Staphylococcus aureus strains[J]. Chinese Journal of Microbiology and Immunology, 2015, 35(1):46-50. (in Chinese)
[27] VANDENDRIESSCHE S, VANDERHAEGHEN W, LARSEN J, et al. High genetic diversity of methicillin-susceptible Staphylococcus aureus (MSSA) from humans and animals on livestock farms and presence of SCCmec remnant DNA in MSSA CC398[J]. J Antimicrob Chemother, 2013, 69(2):355-362.
[28] 闫虹, 苟建军, 张志坚, 等. 河南地区耐甲氧西林金黄色葡萄球菌SCCmec分型与耐药性分析[J]. 中华医院感染学杂志, 2016, 26(3):512-515.
YAN H, GOU J J, ZHANG Z J, et al. Antibiotic resistance and staphylococcal chromosomal cassette mec typing of methicillin-resistant Staphylococcus aureus isolates in Henan[J]. Chinese Journal of Nosocomiology, 2016, 26(3):512-515. (in Chinese)
[29] 王晓,俞英. 中国北方奶牛金葡菌乳房炎感染现状及耐药性和流行类型研究进展[J]. 畜牧兽医学报, 2015, 46(9):1477-1488.
WANG X,YU Y. Infectious status of mastitis in dairy cattle induced by Staphylococcus aureus and its advances on epidemiological patterns and antimicrobial resistance in Northern China[J]. Acta Veterinaria et Zootechnica Sinica, 2015, 46(9):1477-1488. (in Chinese)
[30] SAMUTELA M T, KALONDA A, MWANSA J, et al. Molecular characterisation of methicillin-resistant Staphylococcus aureus (MRSA) isolated at a large referral hospital in Zambia[J]. Pan Afr Med J, 2017, 26:108.
[31] SONG Z, GU F F, GUO X K, et al. Antimicrobial resistance and molecular characterization of Staphylococcus aureus causing childhood pneumonia in Shanghai[J]. Front Microbiol, 2017, 8:455.
[32] XAVIER A R E O, ALMEIDA A C, SOUZA C N, et al. Phenotypic and genotypic characterization of Staphylococcus aureus isolates in milk from flocks diagnosed with subclinical mastitis[J]. Genet Mol Res, 2017, 16(2):gmr16029709.
[33] LI T M, LU H Y, WANG X, et al. Molecular characteristics of Staphylococcus aureus causing bovine mastitis between 2014 and 2015[J]. Front Cell Infect Microbiol, 2017, 7:127.
[34] DHUP V, KEARNS A M, PICHON B, et al. First report of identification of livestock-associated MRSA ST9 in retail meat in England[J]. Epidemiol Infect, 2015, 143(14):2989-2992.

Study on Resistance Characteristics, Virulence Genes and Molecular Classification of Staphylococcus aureus Isolated from Cows in Xinjiang

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HE Xiaolan, ZHAO Yankun, MENG Lu, LIU Huimin, GAO Jiaojiao, ZHENG Nan. Research Progress in Heteroresistance of Staphylococcus aureus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1432-1445.
[2]	LI Pengxu, LI Shijing, SUN Jun, XIANG Wei, ZHAO Miaomiao, HOU Tianmu, LI Huaming, GUANG Min, CHEN Ruige, XU Mengran, WU Xiaomin, JIANG Hexiang, LEI Liancheng, ZHANG Fuxian. Molecular Subtyping and Identification of Streptococcus suis Meningitidis Type 2 and Its Biological Characteristics [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1192-1207.
[3]	Lü Ruoyi, SI Xiaohui, SUN Zhigang, SHI Xiaomin, LIU Xiaoye. Drug Resistance Situation of Streptococcus suis and Prevention Measures of Infections [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4920-4933.
[4]	HU Xiuhua, SUN Zhixin, ZHAO Mengyang, XIE Jiaqi, WANG Min, CHEN Hailiang, GE Xin, LIU Tianlong, WANG Shaolin. Pathogenicity and Resistance Analysis of Enterococcus faecium from Wild Squirrels [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 3012-3021.
[5]	LU Wanqing, ZHAO Shasha, JIANG Songhong, TONG Zhizi, HUANG Danni, GUO Jianhua, WU Junwei, ZHOU Yang. Effect of Staphylococcus aureus on IFN-α Production in BV2 Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2633-2641.
[6]	MAO Yanni, CHANG Jiawei, LI Na, WANG Xin, KANG Xinyun, MA Qiang, MA Liang, WANG Guiqin. Transcriptome Differential Expression Analysis of Staphylococcus aureus in Biofilm State and Planktonic State [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2697-2707.
[7]	DU Yu, PU Xiaofeng, CHEN Xiaomeng, Lü Fenfen, WANG Li, ZHANG Zehua, ZHANG Baojiang, SU Yan. Identification and Evolutionary Analysis of Three Novel SeM Genotypes of Donkey Streptococcus equi subsp. equi Isolates in Xinjiang [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 231-240.
[8]	ZHANG Haixia, ZHANG Zhenbiao, DAI Hegen, SONG Yu, LI Lei, XIA Zhaofei. Analysis of Epidemiological Characteristics of Clinical Staphylococcus pseudintermedius Isolated from Dogs [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2561-2568.
[9]	ZHANG Jing, CAI Ping, HE Yizhuo, CHEN Weitao, HUANG Yuchen, JIANG Hongxia. Prevalence of Antimicrobial Resistance and Virulence Factors in Enterococci from Poultry Farms in Guangdong Province [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2650-2659.
[10]	ZHANG Jinning, QIAN Mengying, TANG Yongjie, MI Siyuan, SHI Kerong, YU Ying. The Adhesion Effect of Staphylococcus aureus Surface Protein A on Dairy Cattle Mammary Epithelial Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1369-1377.
[11]	LÜ Fenfen, MA Xiaohui, WANG Li, MA Chen, ZHANG Baojiang, SU Yan. Immunogenicity Analysis of Three Different Antigen Proteins of Streptococcus equi in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 752-762.
[12]	ZHANG Hang, LI Xinpu, WU Xiaohu, DING Xuezhi, YAN Zuoting, WANG Shengyi, LI Hongsheng. Investigation of Biofilm Formation, Virulence Genes and agr Typing of Staphylococcus aureus from Bovine Mastitis Cases [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(11): 2903-2910.
[13]	MA Qiang, YANG Rui, WAN Jiahong, CHANG Jiawei, WEI Yanqin, WANG Guiqin. Study on β-lactamase and Its Action Mode of Staphylococcus aureus Isolated from Cows in Ningxia [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5): 1138-1148.
[14]	MA Qiang, WANG Yihui, CHANG Jiawei, WAN Jiahong, WEI Yanqin, WANG Guiqin. The Relationship between Cell Wall Thickening of Staphylococcus aureus and Resistance to β-lactams [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 602-611.
[15]	MA Weiwu, ZHOU Xuezhang. Expression of Panton-Valenine Leukocidin from Staphylococcus aureus and Its Effects on Bovine Mammary Epithelial Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(10): 2105-2112.