马链球菌马亚种3种抗原蛋白对小鼠免疫的免疫原性分析

doi:10.11843/j.issn.0366-6964.2021.03.019

Abstract

Abstract: This study aims to evaluate and compare the immunogenicity and protective efficacy of virulence proteins, SrtA, SeM and EAG of Streptococcus equi subsp. equi (S. equi). These 3 recombinant proteins were expressed, purified, and then were used alone or in combination to immunize mice. The mice serum antibody titer, antibody subtype, and immune molecules were detected after immunization. Challenge protection rate, bacterial loading of liver, spleen, kidney, and lung were detected after challenge. In addition, histopathological changes of liver, spleen, kidney, and lung were observed. The ELISA results showed that IgG, IgG1 and IgG2b levels induced by the combined immunization group were higher than those immunized alone group and SrtA immunization group induced higher IgG2a levels than SeM and EAG group. The results of challenge protection rate, bacterial load and histopathological observation showed that the combined immunization group was superior to the single immunization group. The fluorescence quantitative PCR results of immune related molecules showed that the transcription levels of MHCⅠ, TCR, TLR2, TLR3 and TLR4 induced by SrtA were higher than those induced by SeM,EAG immunized group and control group. The MHC Ⅰ and TLR3 induced by combined-immunized group were higher than those of single-immunized group. In conclusion, the SrtA was a better immune stimulation and induction antigen and three proteins immunized in combination has better immunogenicity and provide efficacious immune protection. This data laid theory foundation and experiment basis for the subunit vaccine development of strangles.

Key words: Streptococcus equi, subunit antigen protein, immunogenicity, mice model

CLC Number:

S852.611

LÜ Fenfen, MA Xiaohui, WANG Li, MA Chen, ZHANG Baojiang, SU Yan. Immunogenicity Analysis of Three Different Antigen Proteins of Streptococcus equi in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 752-762.

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Immunogenicity Analysis of Three Different Antigen Proteins of Streptococcus equi in Mice

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