用RT-PCR检测海兰褐蛋鸡禽戊型肝炎病毒

doi:doi: 10.11843/j.issn.0366-6964.2016.08.012

Abstract

Abstract:

We aimed to identify the existence of avian hepatitis E virus (HEV) in broiler breeders in China.In the current study，a total of 40 fecal and 10 serum samples were collected from Hailan brown laying hens suffered from the big liver and spleen disease to detect the avian HEV ORF2 gene by reverse transcription PCR (RT-PCR).The positive PCR products were cloned and sequenced.Artificial inoculation experiment was carried out on two-week-old SPF chickens with the liver of suspected HEV infected chickens.As the result，27 of 40 fecal samples and 4 of 10 serum samples were detected positive for avian HEV RNA.Sequences analysis revealed that 2 HEV RNA positive samples shared 78.1%-98.3% nucleotide sequence identities to avian HEV strain reported in NCBI.Phylogenetic analysis showed that these avian HEVs related to China and Europe isolates belonged to avian HEV genotype 3.All of four SPF chickens were positive of avian HEV helicase gene RNA at 14 days after intravenous inoculation.These results confirmed the existence of avian HEV infection in laying hens in China，which provide experimental research basis of avian HEV.

CLC Number:

S852.659.6

YANG Shu-qing，SUN Jia，KONG Xiang-wei，YUN Lu-xiang，SUN Shu-hong. Detection of Avian Hepatitis E Virus in Hailan Brown Layer Chickens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1618-1622.

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Detection of Avian Hepatitis E Virus in Hailan Brown Layer Chickens

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