gD基因插入增强gI/gE/gM缺失重组伪狂犬病病毒对小鼠的免疫效果

doi:10.11843/j.issn.0366-6964.2026.01.028

Abstract

Abstract:

In this study， we aimed to develop recombinant PRV rPRV-AH-gI^-/gE^-/gM^-/gD⁺ with double gD and evaluate its immune efficacy. The recombinant virus rPRV-AH-gI^-/gE^-/gM^-/gD⁺ was constructed by deleting gM gene and inserting gD expression cassette at the deficient region. Four-week-old Kunming mice were immunized twice with 10^7.0 TCID₅₀ inactivated rPRV-AH-gI^-/gE^-， rPRV-AH-gI^-/gE^-/gM^-， rPRV-AH-gI^-/gE^-/gM^-/gD⁺ at an interval of 3 weeks. Commercial vaccine and DMEM were as the control group. After immunization， regular blood was collected， neutralizing antibody levels in sera were detected by neutralization test， and total antibody levels were detected by ELISA. At 6 weeks of first immunization， mice were challenged with 10^5.5TCID₅₀ PRV-AH. The inserted gD gene was expressed with the molecular weight of 53 ku in the rPRV-AH-gI^-/gE^-/gM^-/gD⁺. The neutralizing and total IgG antibody levels of rPRV-AH-gI^-/gE^-/gM^-/gD⁺ group were significantly higher than those of rPRV-AH-gI^-/gE and rPRV-AH-gI^-/gE^-/gM^- at 4 and 6 weeks after first immunization （P<0.01）. However， compared with the commercial vaccine group， the difference was not significant （P>0.05）. After challenged with PRV-AH， the protection rates were 100%， 70%， 60% and 90% of the rPRV-AH-gI^-/gE^-/gM^-/gD⁺， rPRV-AH-gI^-/gE^-/gM^-， rPRV-AH-gI^-/gE^- and commercial vaccine groups respectively. The results showed that the insertion of gD gene can improve the immune protection effect of the recombinant virus which is helpful in developing vaccine candidate for PRV control.

Key words: pseudorabies virus, double gD, homologous recombination, immune efficacy

CLC Number:

S852.659.1

LU Lingguang, LIU Jiaqi, LU Peiqi, WU Ruibin, HUANG Danni, LIU Jiayue, LIANG Wanqi, JU Chunmei. Immune Effect of gD Gene Insertion-enhanced gI/gE/gM Deleted Recombinant Pseudorabies Virus in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2026, 57(1): 327-336.

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References 31

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引物名称 Gene name	引物序列（5′→3′） Primer sequences
gM-LA1-F	CATCGTTCCCGTCTGGTGTC
gM-LA1-R	TTCGTCGAGGTCGTCGTGTC
gM-RA1-F	AGAGGCTGATGTGGAAGCTGTT
gM-RA1-R	TGATAGAGGTCTGCGGGTTCG
Sosoo-EGFP-F	ACCTGCAGGCATGCAAGCTTCGTTACATAACTTACGGTAAAT
Sosoo-EGFP-R	CCACATCAGCCTCTAAGCTTTAAGATACATTGATGAGTTTGG
Sosoo-gD-F	GCAGAAATCGTCGACCTGCAGGCATGCAAGCTTCAGATATACGCGTTGAC
Sosoo-gD-R	TCCTGGCCAACAGCTTCAACATCAGCCTCTACCTCAGAAG CCATAGAGC

Immune Effect of gD Gene Insertion-enhanced gI/gE/gM Deleted Recombinant Pseudorabies Virus in Mice

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