一株猪伪狂犬病病毒Bartha-K61疫苗变异株的分离鉴定及生物学特性分析

doi:10.11843/j.issn.0366-6964.2025.12.031

Abstract

Abstract: To study the variation of porcine pseudorabies virus (PRV) local isolates, a PRV virus strain named JH2403 was successfully isolated from diseased materials sent to a pig farm in Yantai City. Perform genetic evolution analysis on its gB, gC, gD, TK genes and study of the pathogenicity of the isolates in mice and piglets. Phylogenetic analysis showed that the gB, gC, gD, TK genes of the strain had 99.9% to 100% homology with the vaccine strain Bartha-K61, with a total of 3 amino acid mutations. The pathogenicity test of mice showed that 1×10³ TCID₅₀·mL^-1 virus solution with a concentration of 0.2 mL per mouse was infected, and the mortality rate on the 6th day was 100%. HE staining of brain tissue showed lymphocyte infiltration. Pathogenicity test of piglets proved that there were no deaths although there were obvious symptoms. A strain of porcine pseudorabies virus was successfully isolated, which had the highest homology with the vaccine strain Bartha-K61, which showed enhanced pathogenicity in mice and lower pathogenicity in piglets. This study provides a reference for the genetic evolution analysis and epidemiological study of PRV in Shandong Province.

Key words: pseudorabies virus, separation and identification, genetic variation, pathogenicity

CLC Number:

S852.659.1

HU Wenjia, SHI Jianhao, LI Jiani, YIN Yuchen, ZHANG Xinyue, DONG Haibing, YANG Haiyan, ZHANG Chuanmei. Isolation, Identification and Biological Characterization of a Porcine Pseudorabies Virus Bartha-K61 Vaccine Variant[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(12): 6293-6298.

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Isolation, Identification and Biological Characterization of a Porcine Pseudorabies Virus Bartha-K61 Vaccine Variant

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