马疱疹病毒1型分离毒株对叙利亚金黄地鼠的致病性

doi:10.11843/j.issn.0366-6964.2025.01.030

Abstract

Abstract:

In order to understand the biological characteristics of EHV-1/China/YL2023 isolated from Yili and its pathogenicity to Syrian golden hamsters, the isolates were purified by plaque assay, virus culture and proliferation, saturated ammonium sulfate concentration and sucrose density gradient centrifugation. To investigate the pathogenicity of EHV-1/China/YL2023 virus isolate, Syrian golden hamsters were infected with different doses of EHV-1/China/YL2023 virus by intranasal inoculation. The results showed that the titer of purified EHV-1/China/YL2023 strain reached 10^6.64 TCID₅₀·mL^-1. Syrian mice aged 4-5 weeks infected with EHV-1/China/YL2023 strain showed depression, decreased food intake, drooling, curled up, hunched back, unkily dropped fur and varying degrees of neurological symptoms within 14 days. The average body weight of the recovered hamsters decreased by 25.9%, and the survival rate of the 10⁷-10⁵ TCID₅₀·0.1 mL^-1 dose groups was 50%.The pathological results showed that different doses of virus caused different degrees of damage to the lungs and brains of Syrian mice, including alveolar wall thickening, brain neuron swelling, a large number of neutrophils and lymphocytes infiltration, hemorrhage and congestion. The viral load measurement results of different tissues showed that the Syrian rats contained viral DNA in the lungs, brain and lymph nodes, and the content was high in the lungs and brain. In conclusion, the EHV-1/China/YL2023 isolates were highly pathogenic to Syrian golden hamsters aged 4 to 5 weeks. This study provides a basis for the study of the biological characteristics and pathogenic mechanism of EHV-1, and provides support for the future development of EHV-1 vaccine.

Key words: EHV-1, purification of plaque, multiplication of virus, pathogenicity

CLC Number:

LIU Jianhua, SA Ruixue, ZHANG Siyu, LI Yintao, DENG Zhichao, JIA Handuo, ZHAO Min, FU Yu, YANG Yiming, RAN Duoliang, JA Erken. Pathogenicity of Equine Herpesvirus Type 1 Isolates to Syrian Golden Hamsters[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(1): 327-334.

Figures/Tables 6

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

References 16

1	MARENZONI M L , DE WAURE C , TIMONEY P J . Efficacy of vaccination against equine herpesvirus type 1 (EHV-1) infection: systematic review and meta-analysis of randomised controlled challenge trials[J]. Equine Vet J, 2023, 55 (3): 389- 404. doi: 10.1111/evj.13870
2	MAHMOUD H Y A H , ANDOH K , HATTORI S , et al. Characterization of glycoproteins in equine herpesvirus-1[J]. J Vet Med Sci, 2013, 75 (10): 1317- 1321. doi: 10.1292/jvms.13-0168
3	BARRANDEGUY M , VISSANI A , OLGUIN C , et al. Experimental reactivation of equine herpesvirus-3 following corticosteroid treatment[J]. Equine Vet J, 2008, 40 (6): 593- 595. doi: 10.2746/042516408X333399
4	NEHAL S S. Preliminary trials for the production of equine viral abortion inactivated vaccine[D]. Benha: Virology Benha University, 2006.
5	OLADUNNI F S , HOROHOV D W , CHAMBERS T M . EHV-1: A constant threat to the horse industry[J]. Front Microbiol, 2019, 10, 2668. doi: 10.3389/fmicb.2019.02668
6	MIAO J X , CHARD L S , WANG Z M , et al. Syrian hamster as an animal model for the study on infectious diseases[J]. Front Immunol, 2019, 10, 2329. doi: 10.3389/fimmu.2019.02329
7	车传忠, 鲍子磊, 胡月, 等. 马疱疹病毒1型感染马疾病模型的建立及评价[J]. 中国畜牧兽医, 2021, 48 (6): 2196- 2203.
	CHE C Z , BAO Z L , HU Y , et al. Establishment and evaluation of equine herpesvirus type 1 infection equine disease model[J]. China Animal Husbandry & Veterinary Medcine, 2021, 48 (6): 2196- 2203.
8	胡月, 刘建华, 王雪竹, 等. 马疱疹病毒1型TaqMan实时荧光定量PCR检测方法的建立[J]. 中国兽医学报, 2021, 41 (12): 2345- 2350.
	HU Y , LIU J H , WANG X Z , et al. Establishment of TaqMan real-time PCR for detecting equid Herpesvirus 1[J]. Chinese Journal of Veterinary Science, 2021, 41 (12): 2345- 2350.
9	杨永龙, 刘建华, 宋焕堂, 等. 新疆伊犁地区马疱疹病毒1型的分离与鉴定[J]. 中国预防兽医学报, 2016, 38 (7): 550- 553.
	YANG Y L , LIU J H , SONG H T , et al. Isolation and identification of equine herpesvirus 1 in Xinjiang[J]. Chinese Journal of Preventive Veterinary Medicine, 2016, 38 (7): 550- 553.
10	段汝丽, 佟盼盼, 邓海峰, 等. Ⅷ群神经致病型马疱疹病毒1型的分离及鉴定[J]. 中国预防兽医学报, 2022, 44 (9): 940- 945.
	DUAN R L , TONG P P , DENG H F , et al. Isolation and identification of neuropathogenic equid herpesvirus 1 within group Ⅷ[J]. Chinese Journal of Preventive Veterinary Medicine, 2022, 44 (9): 940- 945.
11	HUSSEY S B , CLARK R , LUNN K F , et al. Detection and quantification of equine herpesvirus-1 viremia and nasal shedding by real-time polymerase chain reaction[J]. J Vet Diagn Invest, 2006, 18 (4): 335- 342. doi: 10.1177/104063870601800403
12	PAILLOT R , SUTTON G , THIEULENT C , et al. New EHV-1 variant identified[J]. Vet Rec, 2020, 186 (17): 573.
13	BURTON E A , WECHUCK J B , WENDELL S K , et al. Multiple applications for replication-defective herpes simplex virus vectors[J]. Stem Cells, 2001, 19 (5): 358- 377. doi: 10.1634/stemcells.19-5-358
14	AWAN A R , CHONG Y C , FIELD H J . The pathogenesis of equine herpesvirus type 1 in the mouse: a new model for studying host responses to the infection[J]. J General Virol, 1990, 71 (5): 1131- 1140. doi: 10.1099/0022-1317-71-5-1131
15	KIM S K , SHAKYA A K , O'CALLAGHAN D J . Immunization with attenuated equine herpesvirus 1 strain KyA induces innate immune responses that protect mice from lethal challenge[J]. J Virol, 2016, 90 (18): 8090- 8104. doi: 10.1128/JVI.00986-16
16	SALEH A G , EL-HABASHI N , ABD-ELLATIEFF H A , et al. Comparative study of the pathogenesis of rhinopneumonitis induced by intranasal inoculation of hamsters with equine herpesvirus-9, equine herpesvirus-1 strain Ab4p and zebra-borne equine herpesvirus-1[J]. J Comp Pathol, 2020, 180, 35- 45. doi: 10.1016/j.jcpa.2020.08.002

[1]	FAN Wei, LIU Xinxin, ZHAI Yilu, ZHANG Xinyu, WANG Wei, FU Jiaqi, SUN Fuliang. Isolation and Identification of Klebsiella pneumoniae of Sheep Origin and Establishment of a Method for the Extraction of Its Outer Membrane Vesicles [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(1): 353-364.
[2]	WANG Hao, WANG Shiyu, XIAO Jinlong, SHEN Jue, PAN Tianling, ZHANG Jingsong, XIAO Peng, GAO Hong. Evolutionary Analysis of High Pathogenicity Virulence Island Structure Gene of Escherichia coli from Pigs and Its Effect on TNF/NF-κB Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(1): 392-403.
[3]	Shan ZHANG, Dahu LIU, Baojing LIU, Lin LIANG, Ruiying LIANG, Xinming TANG, Xusheng QIU, Chan DING, Jiabo DING, Shaohua HOU. Isolation, Identification and Pathogenicity Analysis of a Pigeon Paramyxovirus-1 Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 4051-4060.
[4]	Bilin XIE, Zhimin LIN, Binbin LIN, Yijuan XU, Fengqiang LIN, Lu YAN, Huini WU, Cuiting LI, Haiou ZHOU, Zhaolong LI. Isolation, Identification and Pathogenicity Analysis of Riemerella anatipestifer Strain LC1 and CX1 [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 4196-4203.
[5]	Yan WANG, Yadong GAO, Chenghui JIANG, Qiaoying ZENG. Isolation and Pathogenicity of a Goose Derived Fowl Adenovirus Type 4 [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 4232-4240.
[6]	Fanfan ZHANG, Jiemao LI, Jia TAN, Jiangnan HUANG, Ling WU, Qipeng WEI, Zhaofeng KANG. Research Progress on Avian Metapneumovirus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3344-3353.
[7]	Yudian SUN, Ziyue SONG, Hongliang ZHANG, Zhihua QIN, Hu SHAN, Ruimei YANG. Isolation and ldentification of Duckling Short Beak and Dwarfism Syndrome Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3623-3630.
[8]	Weizhe LIU, Chenggang LUO, Rong YUAN, Yijie LIAO, Yimin WEN, Ying SUN, Enbo YU, Sanjie CAO, Xiaobo HUANG. Isolation and Identification of a Highly Pathogenic Strain of Porcine Epidemic Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(7): 3049-3063.
[9]	Wangqing BAN MA, Xi CHEN, Yi YUE, Yurong SU, Hua YUE, Cheng TANG. Isolation, Identification and Partial Biological Characteristics of a Bovine Respiratory Coronavirus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(7): 3094-3104.
[10]	Bohua LIU, Hanyu FU, Yuheng WANG, Suolangsizhu, Jiaqiang NIU, Yuhua BAO, Jiakui LI, Yefen XU. Isolation, Identification and Genome Analysis of Type B Pasteurella multocida Isolated from Yak in Tibetan Nakchu City [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(7): 3105-3118.
[11]	Kun YANG, Jingwen MA, Xinrui ZHOU, Liezhu LUO, Zhe LIU, Ziqiang HU, Xingchen WU, Libin LIANG, Shimin GAO. Pathogenicity of Three Recombinant Strains of Infectious Bursal Disease Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(6): 2550-2559.
[12]	Ning ZHOU, Cheng TANG, Jia XU, Hua YUE, Xi CHEN. Pathogenicity and Genomic Characteristics of Feline Panleukopenia Virus A91S Variant in Cats [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(6): 2560-2568.
[13]	XIONG Ting, HE Xianming, ZHAO Xiya, ZHUANG Tingting, HUANG Meizhen, LIANG Shijin, YU Chuanzhao, LIANG Xuejing, CHEN Ruiai. Whole Genome Analysis of Three Predominant Epidemic Strains of Chicken Infectious Bronchitis Virus and Their Pathogenicity [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2109-2122.
[14]	ZHENG Rui, LIU Zishi, ZHANG Kangyou, YAN Yong, WEI Ling, ZEREN Wengmu, DINGZE Demi, HUANG Jianjun, WANG Li, WEI Yong. Isolation, Identification and Biological Characterization of Colletotrichum jasminigenum in Stems of Peanuts [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2206-2213.
[15]	QIN Yi, HU Wenjie, FANG Xiaowei, GUO Qian, TIAN Lanxin, LIU Fang, FANG Chun. Effect of Deletion of the Lipoteichoic Acid Synthase ltaS Gene on the Pathogenicity of Listeria monocytogenes [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 670-679.

Pathogenicity of Equine Herpesvirus Type 1 Isolates to Syrian Golden Hamsters

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 16

Related Articles 15

Recommended Articles

Metrics

Comments