基于组学数据筛选康乐黄鸡16周龄体重候选基因与关键通路

doi:10.11843/j.issn.0366-6964.2025.10.011

Abstract

Abstract:

This study aimed to identify functional genes and key signaling pathways related to body weight at 16 weeks of age in Kangle yellow chickens based on omics data, in order to provide a basis for the selection and breeding of local chicken breeds for market weight traits. To detect SNPs significantly associated with body weight at 16-week in 434 Kangle yellow chickens, genome-wide association study (GWAS) was performed using the "Jingxin No. 1" 55K SNP chip. A total of 13 potential SNPs significantly associated with target traits were detected, located on chromosome 3 (81 641 565 bp), chromosome 4 (1 860 041 bp, 33 409 710 bp, 33 716 131 bp, 33 925 946 bp), chromosome 6 (23 563 123 bp, 35 324 210 bp), chromosome 11 (15 294 113 bp, 15 346 007 bp), chromosome 12 (2 503 065 bp, 2 504 749 bp) and chromosome 18 (1 994 686 bp, 2 307 607 bp). The 825 candidate genes near SNP sites were screened. Gene function annotation analysis showed the most significant enrichment of biological processes was motor activity. The significant enrichment of 17 KEGG pathways were found, including tight junction, glycosaminoglycan degradation and metabolic pathways (P < 0.05). Combined with previous transcriptomic sequencing data, and published literature, KCNQ5, P2RY10, ITM2A, RBPMS, PGAM1, CYP17A1, BNIP3, ATMIN, BCO1, TNNC1, GNAI2, MYH1F, MYH1A, MYH10, and PIK3R5 were preliminarily identified as important candidate genes for body weight traits at 16 weeks of age in Kangle yellow chickens. Neuroactive ligand-receptor interaction, metabolic pathway, adrenergic signaling in cardiomyocytes and tight junction were key pathways. In this study, 13 SNPs, 15 key candidate genes and 4 key pathways were preliminarily identified related to the body weight at 16-week of Kangle yellow chicken. These results provided theoretical basis and technical support for molecular marker-assisted breeding of growth traits in Kangle yellow chickens.

Key words: Kangle yellow chicken, SNP, GWAS, body weight traits, candidate gene, gene pathway

CLC Number:

S831.2

MA Jing'e, WAN Shuxing, LEI Wenjing, ZHANG Yingzhi, YU Zixuan, LIU Zige, XU Jiguo. Identification of Candidate Genes and Key Pathways Associated with Body Weight at 16 Weeks of Age in Kangle Yellow Chickens Based on Omics Data[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(10): 4889-4902.

Figures/Tables 7

Fig. 1

Table 1

Table 2

Fig. 2

Fig. 3

Fig. 4

Table 3

Gene functional analysis of key candidate genes"

基因 Gene	染色体 Chromosome	区域/bp Position	功能 Function
TMEM30A	3	80 772 707~80 786 202	TMEM30A在小鼠骨骼肌再生中有重要作用^[28]
KCNQ5	3	81 552 966~81 817 022	猪生长相关候选基因^[29]
P2RY10	4	1 435 115~1 442 506	与阳原驴体尺性状和延边牛生长性状相关^[30-31]
ITM2A	4	1 468 932~1 477 914	参与德州驴骨骼肌的生长和发育^[32]
TBX22	4	1 505 219~1 510 388	Wnt信号通路通过TBX22调节鸡胚上颌骨形态发生和生长^[33]
PHKA1	4	1 863 361~1 879 695	可能参与调节鸡肉早期生长^[34]
SH3D19	4	33 241 343~33 322 918	与斑马鱼胚胎发育、体节发生相关^[35]
RBPMS	4	34 740 148~34 745 550	对于斑马鱼心脏发育具有重要调控作用^[36]
PGAM1	6	23 308 079~23 310 457	参与藏羊细胞增殖以及精子发生^[37]
BTRC	6	23 789 857~23 907 913	与鸡生长性状相关^[11]
CYP17A1	6	24 341 475~24 343 952	参与鸡卵泡和睾丸的生长和发育^[38-39]
SLK	6	25 039 625~25 086 733	与成人肌肉发育有关^[40]
EBF3	6	35 109 889~35 223 910	与鸡胚骨骼发育有关^[41]
BNIP3	6	35 758 032~35 763 615	与动物肌肉发育有关^[42]
ATMIN	11	15 376 764~15 396 951	对于小鼠正常肾脏发育至关重要^[43]
BCO1	11	15 458 803~15 472 637	参与调节鸡成肌细胞的增殖^[44]
CDH13	11	15 966 410~16 399 105	调节牛早期生殖发育^[45]
KCNG4	11	16 658 646~16 675 825	与俄罗斯原住民卡拉柴山羊臀部高度、体长和胸围相关^[46]
TNNC1	12	975 844~981 282	调控鹌鹑肌肉分化和生长性能^[47]
MUSTN1	12	1 237 476~1 240 740	与鸡、鸭肌肉发育有关^[48-49]
GNAI2	12	3 602 465~3 612 992	为绵羊生长相关基因^[50-51]
MYH1F	18	407 274~424 973	在家禽肌肉生成中起关键作用^[52]
MYH1A	18	458 828~477 172	海扬黄鸡生长发育的候选基因^[53]
MYH10	18	1 725 821~1 818 952	可能参与调节鸡肉组织早期生长^[34]
PIK3R5	18	1 984 538~2 033 615	与绵羊体型特征相关的基因^[54]
ARHGAP44	18	2 210 621~2 231 195	调控猪的腰肌面积^[55]

Table 3

References 57

1	谢欣怡,陈俊赫,王文浩.康乐黄鸡肌肉生长抑制素基因多态性对上市日龄体重的遗传效应分析[J].畜牧与兽医,2023,55(10):6-9.
	XIEX Y,CHENJ H,WANGW H.Polymorphism of MSTN gene in Kangle yellow chicken and its genetic effect of market weight of the bird[J].Animal Husbandry and Veterinary Medicine,2023,55(10):6-9.
2	谢鑫峰,钟梓奇,王子轶,等.基于全基因组关联分析研究文昌鸡初生重性状相关的候选基因[J].畜牧与兽医,2024,56(7):1-5.
	XIEX F,ZHONGZ Q,WANGZ Y,et al.Genome-wide association analysis revealed candidate genes related with birthweight traits in Wenchang chicken[J].Animal Husbandry and Veterinary Medicine,2024,56(7):1-5.
3	彭秋玲,汪会文,王䶮,等.利用线粒体CoI基因探讨江西五个地方鸡种的遗传多样性[J].粮油与饲料科技,2022(3):9-13.
	PENGQ L,WANGH W,WANGY,et al.Exploring the genetic diversity of five local chicken breeds in Jiangxi using mitochondrial CoI gene[J].Grain Oil And Feed Technology,2022(3):9-13.
4	曹原, 彭瑞妮, 郑文亚, 等. Diquat诱导的氧化应激对康乐黄鸡睾丸组织结构及睾酮合成相关基因表达的影响[C]. 西安: 中国畜牧兽医学会兽医产科学分会第八届会员代表大会暨第十五次学术研讨会, 2021.
	CAO Y, PENG R N, ZHENG W Y, et al. Effects of Diquat-Induced oxidative stress on testicular tissue structure and testosterone synthesis-related gene expression in Kangle yellow chicken[C]. Xian: The 8th Member Representative Congress and the 15th Academic Symposium of the Veterinary Obstetrics and Gynecology Branch of the Chinese Association of Animal Science and Veterinary Medicine, 2021. (in Chinese)
5	孙汉,欧阳建华,潘珂,等.从万载康乐黄鸡的产蛋规律探讨地方鸡种产蛋性能的选择[J].中国畜牧杂志,2004(8):53-55.
	SUNH,OUYANGJ H,PANK,et al.Exploring the selection of egg-laying performance of local chicken breeds from the egg-laying pattern of Wanzai Kangle yellow chicks[J].Chinese Journal of Animal Science,2004(8):53-55.
6	周洁蕊,马忠文,李园园,等.蚯蚓发酵液对康乐黄鸡生产性能、蛋品质及脂质指标的影响[J].中国兽医学报,2022,42(8):1697-1702.
	ZHOUJ R,MAZ W,LIY Y,et al.Effect of fermented earthworm broth on production performance, egg quality and lipid index of Kangle yellow chicken[J].Chinese Journal of Veterinary Science,2022,42(8):1697-1702.
7	孙汉,欧阳建华,熊建华,等.羽速基因对万载康乐黄鸡蛋用性能的影响[J].动物科学与动物医学,2002(11):12-14.
	SUNH,OUYANGJ H,XIONGJ H,et al.Effect of the feather speed gene on production performance in Wanzai Kangle yellow chicks[J].Swine Industry Science,2002(11):12-14.
8	欧阳建华,熊建华,孙汉,等.羽速基因对万载康乐黄鸡肉用性能的影响[J].江西农业大学学报(自然科学),2002(4):508-512.
	OUYANGJ H,XIONGJ H,SUNH,et al.A study on the effect of the feather speed gene on meat traits in Wanzai Kangle yellow chicks[J].Acta Agriculturae Universitatis Jiangxiensis,2002(4):508-512.
9	杨梦园,宁中华.鸡重要性状GWAS分析的研究进展[J].中国家禽,2023,45(4):105-110.
	YANGM Y,NINGZ H.Research progress on GWAS analysis of important traits in chicken[J].China Poultry,2023,45(4):105-110.
10	杨燕. 京海黄鸡分子标记与生长及屠宰性状关系的研究[D]. 扬州: 扬州大学, 2007.
	YANG Y. Study on the relationship between molecular markers and growth & carcass characteristics in Jinghai yellow chicken[D]. Yangzhou: Yangzhou University, 2007. (in Chinese)
11	ZHANGG X,FANQ C,ZHANGT,et al.Genome-wide association study of growth traits in the Jinghai Yellow chicken[J].Genet Mol Res,2015,14(4):15331-15338. doi: 10.4238/2015.November.30.10
12	WANGW H,WANGJ Y,ZHANGT,et al.Genome-wide association study of growth traits in Jinghai Yellow chicken hens using SLAF-seq technology[J].Animal genetics,2019,50(2):175-176. doi: 10.1111/age.12346
13	ZHANGG X,DINGF X,WANGJ Y,et al.Polymorphism in exons of the myostatin gene and its relationship with body weight traits in the Bian chicken[J].Biochem Genet,2011,49(1-2):9-19. doi: 10.1007/s10528-010-9380-x
14	范晨宇,单艳菊,章明,等.立华麻黄鸡体重和肉品质性状全基因组关联分析[J].畜牧兽医学报,2023,54(12):4982-4992. doi: 10.11843/j.issn.0366-6964.2023.12.010
	FANGC Y,SHANY J,ZHANGM,et al.Genome-wide association study of body weight and meat quality traits in Lihua Mahuang chickens[J].Acta Veterinaria et Zootechnica Sinica,2023,54(12):4982-4992. doi: 10.11843/j.issn.0366-6964.2023.12.010
15	ZHONGC H,LIX C,GUAND L,et al.Age-dependent genetic architectures of chicken body weight explored by multidimensional GWAS and molQTL analyses[J].J Genet Genomics,2024,51(12):1423-1434. doi: 10.1016/j.jgg.2024.09.003
16	WANGJ,LIUJ,LEIQ X,et al.Elucidation of the genetic determination of body weight and size in Chinese local chicken breeds by large-scale genomic analyses[J].BMC Genomics,2024,25(1):296. doi: 10.1186/s12864-024-10185-6
17	樊庆灿,王金玉,张跟喜,等.运用四种线性模型对京海黄鸡上市体重进行全基因组关联分析[J].畜牧兽医学报,2014,45(7):1053-1059. doi: 10.11843/j.issn.0366-6964.2014.07.004
	FANQ C,WANGJ Y,ZHANGG X,et al.A genome-wide association study of market weight using four statistic models in Jinghai yellow chicken[J].Acta Veterinaria et Zootechnica Sinica,2014,45(7):1053-1059. doi: 10.11843/j.issn.0366-6964.2014.07.004
18	NY/T 33-2004, 鸡饲养标准[S].
	NY/T 33-2004, Feeding standard of chicken[S]. (in Chinese)
19	PURCELLS,NEALEB,TODD-BROWNK,et al.PLINK: a tool set for whole-genome association and population-based linkage analyses[J].Am J Hum Genet,2007,81(3):559-575. doi: 10.1086/519795
20	ZHOUX,STEPHENSM.Genome-wide efficient mixed-model analysis for association studies[J].Nat Genet,2012,44(7):821-824. doi: 10.1038/ng.2310
21	YEJ,COULOURISG,ZARETSKAYAI,et al.Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction[J].BMC Bioinformatics,2012,13(1):134. doi: 10.1186/1471-2105-13-134
22	BUD,LUOH,HUOP,et al.KOBAS-i: intelligent prioritization and exploratory visualization of biological functions for gene enrichment analysis[J].Nucleic Acids Res,2021,49(1):317-325.
23	SZKLARCZYKD,KIRSCHR,KOUTROULIM,et al.The STRING database in 2023: protein-protein association networks and functional enrichment analyses for any sequenced genome of interest[J].Nucleic Acids Res,2023,51(D1):D638-D646. doi: 10.1093/nar/gkac1000
24	MAJEEDA,MUKHTARS.Protein-protein interaction network exploration using Cytoscape[J].Methods Mol Biol,2023,2690(1):419-427.
25	马荆鄂,曾配君,万淑敏,等.基于肝脏转录组测序筛选康乐黄鸡开产相关基因和关键通路[J].华北农学报,2025,40(3):207-214.
	MAJ E,ZENGP J,WANS M,et al.Screening of genes and key pathways related to egg-laying in Kangle yellow chickens based on liver transcriptome sequencing[J].Acta Agriculturae Boreali-Sinica,2025,40(3):207-214.
26	马荆鄂,兰岚,刘梓阁,等.基于垂体转录组测序筛选康乐黄鸡开产性状相关基因和信号通路[J].广东农业科学,2025,52(4):73-87.
	MAJ E,LANL,LIUZ G,et al.Pituitary transcriptome analysis of genes and pathways related to traits at the first egg in Kangle yellow chicken[J].Guangdong Agricultural Sciences,2025,52(4):73-87.
27	XIONGX W,ZHOUM,ZHUX N,et al.RNA sequencing of the pituitary gland and association analyses reveal PRKG2 as a candidate gene for growth and carcass traits in Chinese Ningdu yellow chickens[J].Front Vet Sci,2022,9(1):892024.
28	SUNK X,JIANGX Y,LIX,et al.Deletion of phosphatidylserine flippase β-subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration[J].Zool Res,2021,42(5):650-659. doi: 10.24272/j.issn.2095-8137.2021.195
29	SHIL Y,WANGL G,FANGL Z,et al.Integrating genome-wide association studies and population genomics analysis reveals the genetic architecture of growth and backfat traits in pigs[J].Front Genet,2022,13(1):1078696.
30	宋双,常丝雨,赵威森,等.阳原驴P2RY10基因多态性及其与体尺性状的相关分析[J].中国畜牧杂志,2023,59(6):91-94.
	SONGS,CHANGS Y,ZHAOW S,et al.Analysis of P2RY10 gene polymorphism and its correlation with body size traits in Yangyuan donkeys[J].Chinese Journal of Animal Science,2023,59(6):91-94.
31	王卓. 延边牛FABP5、FABP6、DGAT1、USP43、P2RY10和FUBP3多态性与生长性状关联分析[D]. 延吉: 延边大学, 2023.
	WANG Z. Association analysis of polymorphisms FABP5, FABP6, DGAT1, USP43, P2RY10, and FUBP3 genes with growth traits in Yanbian cattle[D]. Yanji: Yanbian University, 2023. (in Chinese)
32	YUJ,YANGG,LIS P,et al.Identification of Dezhou donkey muscle development-related genes and long non-coding RNA based on differential expression analysis[J].Anim Biotechnol,2023,34(7):2313-2323. doi: 10.1080/10495398.2022.2088549
33	SHIMOMURAT,KAWAKAMIM,TATSUMIK,et al.The role of the Wnt signaling pathway in upper jaw development of chick embryo[J].Acta Histochem Cytochem,2019,52(1):19-26. doi: 10.1267/ahc.18038
34	XUEQ,ZHANGG X,LIT T,et al.Transcriptomic profile of leg muscle during early growth in chicken[J].PLoS One,2017,12(3):e0173824. doi: 10.1371/journal.pone.0173824
35	冷平,陈欣,夏佳敏,等.斑马鱼sh3d19基因的结构和表达分析[J].成都医学院学报,2022,17(5):550-555.
	LENGP,CHENX,XIAJ M,et al.Structure and expression analysis of sh3d19 gene in zebrafish[J].Journal of Chengdu Medical College,2022,17(5):550-555.
36	黄姣, 霍锦倩, 刘姣, 等. RBPMS基因在斑马鱼心脏早期发育过程中的作用初步研究[C]. 西安: 2018年中国水产学会学术年会, 2018.
	HUANG J, HUO J Q, LIU J, et al. Preliminary study of RBPMS gene on the cardiacdevelopment of zebrafish[C]. Xian: 2018 Annual Meeting of the China Society of Fisheries, 2018. (in Chinese)
37	ANX J,LIT T,CHENN N,et al.PGAM1 regulates the glycolytic metabolism of SCs in tibetan sheep and its influence on the development of SCs[J].Gene,2021,804(1):145897.
38	LIC,CAOY F,RENY G,et al.The adiponectin receptor agonist, AdipoRon, promotes reproductive hormone secretion and gonadal development via the hypothalamic-pituitary-gonadal axis in chickens[J].Poult Sci,2023,102(2):102319. doi: 10.1016/j.psj.2022.102319
39	冯宇. 绵羊CYP17A1基因克隆分析及其在睾丸表达的研究[D]. 长春: 吉林农业大学, 2024.
	FENG Y. Cloning analysis of sheep CYP17A1 genes and their expression in the testes[D]. Changchun: Jilin Agricultural University, 2024. (in Chinese)
40	CHRISTOPHERJ S,KHALIDN A,ROSHANS,et al.Distinct roles for Ste20-like kinase SLK in muscle function and regeneration[J].Skelet Muscle,2013,3(1):16. doi: 10.1186/2044-5040-3-16
41	EL-MAGDM A,ALLENS,MCGONNELLI,et al.Bmp4 regulates chick Ebf2 and Ebf3 gene expression in somite development[J].Dev Growth Differ,2013,55(8):710-722. doi: 10.1111/dgd.12077
42	OOSTL J,KUSTERMANNM,ARMANIA,et al.Fibroblast growth factor 21 controls mitophagy and muscle mass[J].J Cachexia Sarcopenia Muscle,2019,10(3):630-642. doi: 10.1002/jcsm.12409
43	GOGGOLIDOUP,HADJIRINN F,BAKA,et al.Atmin mediates kidney morphogenesis by modulating Wnt signaling[J].Hum Mol Genet,2014,23(20):5303-5316. doi: 10.1093/hmg/ddu246
44	PRAUDC,AHMADIEHS A,VOLDOIREE,et al.Beta-carotene preferentially regulates chicken myoblast proliferation withdrawal and differentiation commitment via BCO1 activity and retinoic acid production[J].Exp Cell Res,2017,358(2):140-146. doi: 10.1016/j.yexcr.2017.06.011
45	COENS,KEOGHK,LONERGANP,et al.Early life nutrition affects the molecular ontogeny of testicular development in the young bull calf[J].Sci Rep,2023,13(1):6748. doi: 10.1038/s41598-022-23743-3
46	EASAA A,SELIONOVAM,AIBAZOVM,et al.Identification of genomic regions and candidate genes associated with body weight and body conformation traits in Karachai goats[J].Genes (Basel),2022,13(10):1773. doi: 10.3390/genes13101773
47	PARKJ W,LEEJ H,KIMS W,et al.Muscle differentiation induced up-regulation of calcium-related gene expression in quail myoblasts[J].Asian-Australas J Anim Sci,2018,31(9):1507-1515. doi: 10.5713/ajas.18.0302
48	GUS,HUANGQ,JIEY C,et al.Transcriptomic and epigenomic landscapes of muscle growth during the postnatal period of broilers[J].Journal of Animal Science and Biotechnology,2024,15(5):1851-1865.
49	WANGZ X,LIANGW S,LIX X,et al.Characterization and expression of MUSTN1gene from different duck breeds[J].Anim Biotechnol,2022,33(4):723-730. doi: 10.1080/10495398.2020.1828905
50	YANGP F,SHANGM Y,BAOJ J,et al.Whole-genome resequencing revealed selective signatures for growth traits in Hu and Gangba sheep[J].Genes (Basel),2024,15(5):551. doi: 10.3390/genes15050551
51	杨培福. 基于全基因组重测序筛选绵羊生长性状候选基因[D]. 北京: 中国农业科学院, 2024.
	YANG P F. Screening of sheep growth traits based on whole-genome resequencing candidate gene[D]. Beijing: Chinese Academy of Agricultural Sciences, 2024. (in Chinese)
52	RENP,CHENM Y,LIJ J,et al.MYH1F promotes the proliferation and differentiation of chicken skeletal muscle satellite cells into myotubes[J].Anim Biotechnol,2023,34(7):3074-3084. doi: 10.1080/10495398.2022.2132953
53	YINX M,WUY L,ZHANGS S,et al.Transcriptomic profile of leg muscle during early growth and development in Haiyang yellow chicken[J].Arch Anim Breed,2021,64(2):405-416. doi: 10.5194/aab-64-405-2021
54	KOMINAKISA,HAGER-THEODORIDESA L,ZOIDISE,et al.Combined GWAS and 'guilt by association'-based prioritization analysis identifies functional candidate genes for body size in sheep[J].Genet Sel Evol,2017,49(1):41. doi: 10.1186/s12711-017-0316-3
55	LUANM H,RUAND L,QIUY B,et al.Genome-wide association study for loin muscle area of commercial crossbred pigs[J].Anim Biosci,2023,36(6):861-868. doi: 10.5713/ab.22.0407
56	罗威,郑茗,唐盈盈,等.中国地方家鸡群体遗传与表型多样性及其保护现状[J].养禽与禽病防治,2023(8):29-35.
	LUOW,ZHENGM,TANGY Y,et al.Genetic and phenotypic diversity and conservation status of native Chinese domestic chicken populations[J].Poultry Husbandry and Disease Control,2023(8):29-35.
57	CARLBORGO,KERJES,SCHVTZK,et al.A global search reveals epistatic interaction between QTL for early growth in the chicken[J].Genome Res,2003,13(3):413-421. doi: 10.1101/gr.528003

染色体 Chromosome	物理位置/bp Position	P值 P-value	基因型(个体数) Genotype (Number)	基因型频率 Genotype frequency	16周龄体重(平均值±标准差)/g Body weight at 16 weeks (Mean±standard deviation)
3	81 641 565	4.00×10^-8	CC (386)	0.970	790.73±138.97^a
			TC (12)	0.030	920.83±80.26^b
4	1 860 041	2.88×10^-9	CC (376)	0.945	968.09±135.34^a
			TC (21)	0.053	988.10±177.89^a
			TT (1)	0.002	1 000^b
4	33 409 710	2.83×10^-6	CC (385)	0.967	970.78±138.95^a
			TC (13)	0.033	923.08±86.86^b
4	33 716 131	2.83×10^-6	GG (385)	0.967	970.78±138.95^a
			AG (13)	0.033	923.08±86.86^b
4	33 925 946	2.83×10^-6	TT (385)	0.967	970.78±138.95^a
			CT (13)	0.033	923.08±86.86^b
6	23 563 123	6.00×10^-7	GG(8)	0.020	969.74±138.33^a
			AG (390)	0.980	943.75±107.35^b
6	35 324 210	7.68×10^-8	AA (1)	0.002	1 000^a
			AC (21)	0.053	973.81±111.93^b
			CC (376)	0.945	968.88±139.30^ab
11	15 294 113	3.48×10^-7	TT(386)	0.969	970.73±138.45^a
			CT(12)	0.031	920.83±105.00^b
11	15 346 007	3.48×10^-7	CC(386)	0.969	970.73±138.45^a
			TC(12)	0.031	920.83±150.00^b
12	2 503 065	1.79×10^-12	GG(2)	0.005	950±50^a
			GT(10)	0.027	995±145.69^b
			TT(358)	0.968	968.65±137.86^c
12	2 504 749	1.79×10^-12	AA(358)	0.968	968.65±137.86^a
			CA(10)	0.027	995±145.69^b
			CC(2)	0.005	950±50^c
18	1 994 686	1.30×10^-7	TT(387)	0.972	970.80±138.14^a
			CT(11)	0.028	913.64±113.00^b
18	2 307 607	5.84×10^-9	AG(8)	0.020	925±129.90^a
			GG(390)	0.980	970.13±137.84^b

引物名称 Primer name	SNP位置/bp Position	方向 Direction	引物序列(5′→3′) Primer sequence	退火温度/℃ Temperature	片段长度/bp Length
1	Chr3:81 641 565	Forward	CATGCAAAGCACCGAACTCC	60	233
		Reverse	AGAAAAGCGCTCCAGTGTCT
2	Chr4:1 860 041	Forward	CTGGGTCCCTATATCCACATACATT	61	418
		Reverse	GCCAGCTGGACAAAAGCCTG
3	Chr4:33 409 710	Forward	TAAAGTTGAGGTGCTTGACC	55	322
		Reverse	TGGACAGTTACACAGAACAT
4	Chr4:33 716 131	Forward	GGACATTTGCACACACCGTT	60	280
		Reverse	TTGCTTGCTCCTGCTAGCTC
5	Chr4:33 925 946	Forward	TACGTTCAGAGTGACGTTTGTATG	60	393
		Reverse	CTTCAAGCCAGGATGGAGCAT
6	Chr6:23 563 123	Forward	GTGAACGTGTCAGCCAACTG	60	372
		Reverse	GCCTGAAGACAAGCGCATAG
7	Chr6:35 324 210	Forward	GTTGGCAATGGGGTGTTTGA	59	280
		Reverse	CCTGGGTTCTGGGCTAACTT
8	Chr11:15 294 113	Forward	CATATCAGCACCCCAAGCCTC	60	297
		Reverse	TGCAGAAAACACCTACAGCTTC
9	Chr11:15 346 007	Forward	ATCTCAGCCACAGTGGTCCT	61	235
		Reverse	AGTATGGGGGCAGTTTGGGA
10	Chr12:2 503 065	Forward	TTTCCAGTTGTTCCAGCTGAC	59	295
		Reverse	TGCCAACCTGAAAGGTCTTCT
11	Chr12:2 504 749	Forward	GTGCCTTGAGCTAGCATTGTG	61	523
		Reverse	TTTCAGACATGCCGTGCGTT
12	Chr18:1 994 686	Forward	GCTCACATCATGCAGAGAAACA	60	534
		Reverse	CCAGGCCAAGACACTGGAAG
13	Chr18:2 307 607	Forward	TGTCAAAGCCAACTCCCTCTT	60	339
		Reverse	GACAGCGTCACTTCCCACG

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Identification of Candidate Genes and Key Pathways Associated with Body Weight at 16 Weeks of Age in Kangle Yellow Chickens Based on Omics Data

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