母猪死胎和木乃伊全基因组关联分析

doi:10.11843/j.issn.0366-6964.2025.03.023

Abstract

Abstract:

The aim of this research was to identify candidate genes and genetic markers associated with stillbirth and mummified fetus traits in the first litter of Large White sows. A total of 1 805 Large White sows were genotyped using the "Zhongxin No. 1" 50K SNP chip. After filtering and selecting the data, a genome-wide association study (GWAS) was conducted, incorporating phenotypic data of stillbirths and mummified fetuses from the first litter. The results showed that 24 SNPs were significantly associated with the number of stillbirths, and 18 SNPs were significantly associated with mummified fetus traits, identifying a total of 34 candidate genes. Among these, 25 genes were related to stillbirths (such as SIL1, FSTL4, DHX38, PLK2, PDE4D, etc.), while 9 genes were associated with mummified fetus traits (including LRRTM4, ERC2, ARHGEF3, U6, etc.). A comparison with the Pig QTL database (Pig QTLdb) revealed that some of the candidate genes were located within previously reported genomic regions associated with stillbirth and mummified fetus traits, and significant SNPs exhibited linkage disequilibrium. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses indicated that the genes annotated upstream and downstream of the significant SNPs were closely associated with biological processes such as immune responses, cell signaling, apoptosis and cell division, as well as disease development. This study screened the candidate genes related to the traits of stillbirth and mummy in Large White pig population, and provided an important reference for molecular breeding.

Key words: sow, first parity, the number of stillborn, mummy, GWAS, SNP, QTL

CLC Number:

S828.3

ZHOU Taizeng, YANG Yiting, ZHU Yuehua, QIAN Hongxi, LIU Yihui, GAN Mailin, ZHU Li, SHEN Linyuan. Genome-wide Association Study of Stillbirths and Mummies in Sows[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(3): 1231-1241.

Figures/Tables 8

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References 39

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性状 Trait	窝数 The number of nests	均值 Mean	变异系数 Coefficient of variation	标准差 Standard deviation	最小值 Minimum	最大值 Maximum
死胎Stillborn	1 805	1.089	1.398	1.522	0	14
木乃伊Mummy	1 805	0.463	2.658	1.229	0	18

SNP	染色体 Chromosome	位置 Position	频率 Frequency	效应 Effect	P-value	等位基因 Allele gene	候选基因 Candidate gene
CNC10015399	1	266284487	0.365	0.218	1.690×10^-4	T/C	MAPKAP1
CNC10022904	2	136056964	0.238	0.228	4.817×10^-4	A/G	FSTL4
CNC10022911	2	136443463	0.270	0.237	1.188×10^-4	A/G	TCF7、SKP1
CNCB10002244	2	140800714	0.077	0.462	1.288××10^-5	G/A	CTNNA1、LRRTM2
CNCB10002245	2	140818901	0.077	0.462	1.250×10^-5	C/T	CTNNA1、LRRTM2
CNC10023000	2	140880523	0.077	0.462	1.288×10^-5	A/G	CTNNA1、SIL1
CNCB10002246	2	140914537	0.077	0.462	1.288×10^-5	G/T	SIL1
CNC10042275	4	111347753	0.244	0.230	3.443×10^-4	C/T	FNDC7、HENMT1
CNCB10004339	6	14980246	0.380	0.217	2.687×10^-4	C/T	DHODH、TXNL4B、DHX38、PMFBP1
CNC10071783	7	80726302	0.240	-0.260	3.804×10^-4	A/G	RYR3
CNC10092856	9	130414550	0.358	-0.204	4.193×10^-4	A/G	RPS6KC1、ANGEL2
CNC10092857	9	130527089	0.358	-0.204	4.193×10^-4	A/G	VASH2、LVCR1
CNC10100524	10	20759440	0.094	0.321	4.534×10^-4	G/A	LHX9、C1orf53
CNC10160815	16	37286024	0.185	-0.318	6.683×10^-5	T/C	PLK2
CNCB10011377	16	38483053	0.211	-0.287	1.049×10^-4	G/T	PDE4D
CNC10161206	16	56466146	0.072	0.399	1.833×10^-4	T/C	TENM2
CNC10180344	18	16144023	0.057	0.444	3.188×10^-4	C/T	CHCHD3
CNCB10012226	18	48373518	0.288	0.206	4.401×10^-4	T/A	FAM221A

SNP	染色体 Chromosome	位置 Position	频率 Frequency	效应 Effect	P-value	等位基因 Allele gene	候选基因 Candidate gene
CNC10021238	2	60582816	0.371	0.154	3.458×10^-4	G/A	MYO9B、HAUS8
CNC10013054	3	65253390	0.237	0.208	3.825×10^-5	T/C	U1
CNC10031386	3	66636195	0.194	0.205	1.437×10^-4	T/C	LRRTM4
CNCB10007328	10	8008894	0.275	-0.171	3.907×10^-4	A/G	SPATA17
CNC10130837	13	38240463	0.071	0.322	2.214×10^-4	A/G	ERC2
CNC10130848	13	38604017	0.071	0.322	2.214×10^-4	G/C	ARHGEF3、U6
CNCB10008586	13	40637583	0.243	0.172	4.920×10^-4	T/C	CFAP20DC

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Genome-wide Association Study of Stillbirths and Mummies in Sows

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