Acta Veterinaria et Zootechnica Sinica ›› 2025, Vol. 56 ›› Issue (7): 3484-3494.doi: 10.11843/j.issn.0366-6964.2025.07.040
• Basic Veterinary Medicine • Previous Articles Next Articles
ZHAO Yanping1,2(), WANG Xiaoping3, YU Jialin1,2, GONG Zhaoqian1,2, WU Xiaoling1,2, DENG Guangcun1,2,*(
)
Received:
2024-09-19
Online:
2025-07-23
Published:
2025-07-25
Contact:
DENG Guangcun
E-mail:3414153939@qq.com;dgc@nxu.edu.cn
CLC Number:
ZHAO Yanping, WANG Xiaoping, YU Jialin, GONG Zhaoqian, WU Xiaoling, DENG Guangcun. Role of Fatty Acid-Binding Protein 4 on Chemokine Secretion in Bacillus Calmette-Guérin Infected Macrophages[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(7): 3484-3494.
Table 1
Primer sequence (Mus musculus)"
引物名称 Primer name | 引物序列(5′→3′) Sequence |
CCL2 | F: GCCTGCTGTTCACAGTTGC |
R: GAGTGGGGCGTTAACTGCAT | |
CCL3 | F: GATTCCACGCCAATTCATCG |
R: TTCAGTTCCAGGTCAGTGATG | |
CXCL2 | F: AATGCCTGAAGACCCTGC |
R: TTTTGACCGCCCTTGAGAG | |
CXCL10 | F: TTGCCCTTGGTCTTCTGA |
R: GTCGCACCTCCACATAGC | |
CXCL11 | F: CGGCTGCGACAAAGTTGAAG |
R: TATGAGGCGAGCTTGCTTGG | |
β-Actin | F: GTGCTATGTTGCTCTAGACTTCG |
R: ATGCCACAGGATTCCATACC |
Fig. 1
Effects of vary dose BCG infection on the expression of FABP4 and chemokine in RAW264.7 macrophages A. Expression of FABP4 in macrophages RAW264.7 were detected by Western blot; B-E. mRNA expression of CCL2, CXCL10, CXCL11, CCL3 and CXCL2 in macrophages RAW264.7 were detected by qRT-PCR; F-G. The expression of RAW264.7 CCL3 and CXCL2 proteins in macrophages was detected by ELISA; ns. P > 0.05, *. P < 0.05, **. P < 0.01, ***. P < 0.001"
Fig. 2
Effects of BCG infection for varied time points on the expression of FABP4 and chemokine in RAW264.7 macrophages A. Expressions of FABP4 in macrophages RAW264.7 were detected by Western blot at different BCG infection times; B-E: mRNA expressions of CCL2, CXCL10, CXCL11, CCL3 and CXCL2 in macrophages RAW264.7 were detected by qRT-PCR at different BCG infection times; F-G: ELISA was used to detect the difference of expression of RAW264.7 CCL3 and CXCL2 proteins in macrophages at different times of BCG infection; ns. P > 0.05, *. P < 0.05, **. P < 0.01, ***. P < 0.001"
Fig. 3
Effect of si-FABP4 on chemokines expression of RAW264.7 in BCG-infected macrophages A-E. mRNA expression differences of CCL2, CXCL10, CXCL11, CCL3 and CXCL2 in macrophages RAW264.7 infected with BCG treated with si-FABP4 were detected by qRT-PCR; F-G. The expression levels of RAW264.7 CCL3 and CXCL2 proteins in macrophages infected with BCG treated with si-FABP4 were detected by ELISA; ns. P > 0.05, *. P < 0.05, **. P < 0.01, ***. P < 0.001"
Fig. 5
Effect of BMS-309403 treatment on cytokine/chemokine expression in lung tissue of mice infected with BCG A. Intraperitoneal injection of BMS-309403 and airway perfusion of BCG infected mouse model construction process; B. Representative Western blot images of CCL3, CXCL2, IL-1β, IL-18 proteins expression in lung of mice with BCG infection for 7 days; C-F. Histogram of gray value of protein expression; (magnification, 200×). *. P < 0.05, **. P < 0.01, ***. P < 0.001"
Fig. 6
Effect of FABP4 on inflammatory infiltration and bacterial load in lung tissue after BCG Infection in mice A. Histomorphological evaluation of mouse lung tissue; B. HE staining representation of mouse lung tissue (magnification: 200×) (Arrow points to the positive part); C. Quantitative analysis diagram of mouse lung bacterial load; ***. P < 0.001"
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