木犀草素对秦川牛精子冷冻保存的影响

doi:10.11843/j.issn.0366-6964.2025.07.018

Abstract

Abstract:

The aim of this experiment was to investigate the effect of adding luteolin (LUT) to the diluent on the cryopreservation effect of semen from Qinchuan cattle. In this study, fresh semen was collected from 6 Qinchuan breeding bulls aged 3-5 years, weighing (586±20) kg and in good health. The tested semen was mixed, diluted with freeze-diluent with different concentrations of LUT added, and frozen for preservation. According to the different concentrations of LUT added in the cryodilution solution, this experiment was divided into 5 groups: 0 mg·mL^-1 LUT group (control group), 0.02 mg·mL^-1 LUT group, 0.04 mg·mL^-1 LUT group, 0.08 mg·mL^-1 LUT group and 0.1 mg·mL^-1 LUT group, with 3 replicates set in each group. After thawing, sperm motility was measured using a bovine automatic sperm quality analyser; plasma membrane integrity was detected using the sperm hypotonic swelling test; sperm acrosome integrity and mitochondrial viability were detected using fluorescent staining; and spermatozoa were tested for catalase (CAT) activity, glutathione peroxidase (GSH-Px) activity, and malondialdehyde (MDA) content using a kit. Compared with the control group, sperm viability, linear motility speed, plasma membrane integrity and acrosome integrity were significantly increased in the 0.02 mg·mL^-1 LUT and 0.04 mg·mL^-1 LUT groups (P < 0.05). Meanwhile, sperm mitochondrial viability, CAT and GSH-Px activity in the 0.02 mg·mL^-1 LUT group, 0.04 mg·mL^-1 LUT group and 0.08 mg·mL^-1 LUT group were significantly increased (P < 0.05), and MDA content was significantly decreased (P < 0.05) in all experimental groups. The optimum amount of LUT added was 0.02 mg·mL^-1. The addition of LUT to the diluent could significantly improve the antioxidant capacity of spermatozoa in bovine semen, protect the structural integrity of spermatozoa, alleviate the damage to spermatozoa caused by the freezing-thawing process, and then enhance the motility of spermatozoa after thawing, thus effectively improving the quality of the frozen semen.

Key words: Qinchuan cattle, luteolin, semen, cryopreservation

CLC Number:

S823.3

WU Silin, YANG Benshun, YE Miaomiao, LIANG Entang, LI Fuqiang, MA Weidong, ZAN Linsen, ZHAO Chunping, YANG Wucai. Effect of Luteolin on Semen Cryopreservation of Qinchuan Bull[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(7): 3244-3251.

Figures/Tables 8

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References 25

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木犀草素浓度/(mg·mL^-1) LUT concentration	精子活率/% Motility	精子活力/% Promotility	精子的前向运动平均速率/(μm·s^-1) Velocity
0	50.70±0.70^c	39.45±0.15^b	29.00±3.08^b
0.02	62.45±1.42^a	47.79±1.43^a	34.00±2.12^a
0.04	58.27±0.25^b	48.33±1.12^a	33.60±2.60^a
0.08	53.09±2.62^c	40.70±2.23^b	31.60±2.60^ab
0.1	52.55±2.75^c	40.45±2.20^b	32.20±1.09^ab

木犀草素浓度/(mg·mL^-1) LUT concentration	质膜完整性/% Plasma membrane integrity
0	50.23±0.85^c
0.02	58.91±0.58^a
0.04	56.35±1.95^b
0.08	52.07±0.64^c
0.1	50.55±0.59^c

木犀草素浓度/(mg·mL^-1) LUT concentration	顶体完整率/% Acrosome integrity
0	56.22±2.69^c
0.02	68.93±2.05^a
0.04	68.27±2.23^a
0.08	61.39±1.07^b
0.1	59.32±2.63^bc

木犀草素浓度/(mg·mL^-1) LUT concentration	线粒体活率/% Mitochondrial activity
0	48.08±0.13^d
0.02	56.23±1.11^a
0.04	53.78±0.51^b
0.08	51.93±0.48^c
0.1	49.13±0.50^d

木犀草素浓度/(mg·mL^-1) LUT concentration	过氧化氢酶活性 /(U·mL^-1) CAT	谷胱甘肽过氧化物酶活性/ (U·mL^-1) GSH-Px	丙二醛含量/ (mol·mL^-1) MDA
0	64.32±22.18^c	51.02±0.31^d	1.17±0.04^a
0.02	238.92±21.70^a	60.68±0.48^a	0.55±0.01^cd
0.04	154.40±16.03^b	59.54±0.94^ab	0.44±0.04^d
0.08	164.14±16.51^b	55.89±0.46^bc	0.67±0.09^c
0.1	74.18±20.01^c	52.88±5.95^cd	0.86±0.14^b

Effect of Luteolin on Semen Cryopreservation of Qinchuan Bull

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