丝胶蛋白对承德无角黑山羊精液冷冻保存的影响

doi:10.11843/j.issn.0366-6964.2024.10.019

Abstract

Abstract:

The aim of this study was to explore the effect of sericin on cryopreservation of semen of Chengde hornless black goat. Semen samples of 3 healthy Chengde hornless black goats were collected by the sham vaginal method and randomly divided into 6 groups: control group and experimental groups with different sericin concentrations (0.2%, 0.4%, 0.6%, 0.8%, 1%) added to the OptiXcell 2 dilution. Each group was set up with 5 replicates. Sperm motility, acrosome integrity rate, plasma membrane integrity rate, total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) activity, malondialdehyde (MDA) content, mitochondrial membrane potential (MMP) and ATP content were detected after freezing and thawing. The results showed that the addition of different concentrations of sericin to the semen frozen dilution of Chengde hornless black goat could improve the quality of sperm after freezing and thawing. Compared with other concentrations, the addition of 0.6% sericin significantly increased sperm motility, acrosome integrity rate, plasma membrane integrity rate, mitochondrial membrane potential and ATP content after sperm freezing and thawing, and increased the activities of antioxidant enzymes T-AOC, GSH-Px, SOD and CAT to (13.61±0.39) mmol·g^-1, (26.52±1.30) U·mg^-1, (165.45±11.10) U·mg^-1 and (13.76 ±0.30) U·mg^-1, respectively. Meanwhile, the MDA value was reduced to (3.15±0.20) μmol·mg^-1. In summary, sericin at different concentrations can improve the quality of frozen semen of Chengde hornless black goat, and the most suitable concentration is 0.6%.

Key words: sericin, Chengde hornless black goat, antioxidants, semen freezing

CLC Number:

S827.3

Wentao ZHANG, Yang YU, Yatian QI, Wei ZHAO, Hongjun ZHANG, Chenyu TAO, Wei XIA, Junjie LI. Effect of Sericin on Cryopreservation of Semen of Chengde Hornless Black Goat[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(10): 4466-4474.

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References 41

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丝胶蛋白浓度/% Sericin concentration	精子活力/% Sperm viability
0	42.39±1.23^e
0.2	45.66±1.87^cd
0.4	50.22±1.33^b
0.6	54.42±1.33^a
0.8	47.12±1.17^c
1	44.15±1.49^d

丝胶蛋白浓度/% Sericin concentration	总抗氧化能力/(mmol·g^-1) T-AOC	谷胱甘肽过氧化物酶/ (U·mg^-1) GSH-Px	丙二醛/ (μmol·mg^-1) MDA	超氧化歧化酶/ (U·mg^-1) SOD	过氧化氢酶/ (U·mg^-1) CAT
0	5.90±0.14^c	18.34±1.38^d	7.01±0.52^e	105.12±10.56^e	3.64±0.20^d
0.2	7.48±0.23^c	21.66±1.37^c	5.46±0.36^d	121.34±9.94^d	7.14±0.16^c
0.4	10.09±0.45^b	24.75±1.22^b	3.73±0.12^b	140.87±10.45^b	9.46±0.33^b
0.6	13.61±0.39^a	26.52±1.30^a	3.15±0.20^a	165.45±11.10^a	13.76±0.30^a
0.8	12.23±0.67^a	23.03±0.10^c	3.92±0.26^b	138.12±10.37^bc	9.54±0.20^b
1	8.90±0.33^b	22.84±1.13^c	4.88±0.14^c	128.41±7.27^cd	7.10±0.18^c

丝胶蛋白浓度/% Sericin concentration	ATP/(μmol·mL^-1) ATP content
0	7.00±0.33^d
0.2	10.87±0.46^c
0.4	13.26±0.24^b
0.6	15.31±0.52^a
0.8	13.46±0.27^b
1	11.18±0.32^c

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Effect of Sericin on Cryopreservation of Semen of Chengde Hornless Black Goat

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