猪胚胎冷冻保存的研究进展

doi:10.11843/j.issn.0366-6964.2024.11.002

Abstract

Abstract:

Cryopreservation of embryos is important for long-distance embryo transfer and conservation of genetic resources. However, porcine embryos are sensitive to low temperatures due to their high cytoplasmic lipid content, which increases the difficulty of their preservation. Studies have indicated that techniques such as reducing lipid content and optimizing medium composition can significantly enhance the efficiency of porcine embryo cryopreservation. Additionally, protecting the cytoskeleton and restoring mitochondrial function are also crucial strategies that contribute to this advancement. These improvements collectively foster the broader application and sustainable development of this critical technology.Therefore, this article introduces embryo cryopreservation and summaries multiple methods and measures to improve embryo freezing efficiency. Meanwhile, the writers'team will focus more on the mechanism of cryopreservation of porcine embryos by discussing the intrinsic properties of embryos and the effects of cryopreservation on transcriptome alteration to help people better understand and advance research on porcine embryo cryopreservation.

Key words: porcine, embryo, cryopreservation, vitrification

CLC Number:

S828.3

Jianhua DONG, Xiaoyi FENG, Baigao YANG, Chongyang LI, Hongmei PAN, Lihua LÜ, Xueming ZHAO. Advances in Cryopreservation of Porcine Embryo[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 4796-4807.

Figures/Tables 3

Table 1

Table 2

Fig. 1

References 113

1	LÓPEZA,DUCOLOMBY,CASASE,et al.Effects of porcine immature oocyte vitrification on actin microfilament distribution and chromatin integrity during early embryo development in vitro[J].Front Cell Dev Biol,2021,9,636765. doi: 10.3389/fcell.2021.636765
2	MARTINEZE A,CUELLOC,PARRILLAI,et al.Design, development, and application of a non-surgical deep uterine embryo transfer technique in pigs[J].Anim Front,2013,3(4):40-47. doi: 10.2527/af.2013-0032
3	BLOCKEELC,CAMPBELLA,COTICCHIOG,et al.Should we still perform fresh embryo transfers in ART?[J].Hum Reprod,2019,34(12):2319-2329. doi: 10.1093/humrep/dez233
4	CHENZ J,SHIY H,SUNY,et al.Fresh versus frozen embryos for infertility in the polycystic ovary syndrome[J].N Engl J Med,2016,375(6):523-533. doi: 10.1056/NEJMoa1513873
5	高峰,何琪富,吴盛辉,等.哺乳动物配子冷冻保存并应用于珍稀濒危动物保护的技术策略[J].畜牧兽医学报,2022,53(8):2479-2489. doi: 10.11843/j.issn.0366-6964.2022.08.007
	GAOF,HEQ F,WUS H,et al.Mammalian gametes cryopreserved and applied to technical strategies for the protection of rare and endangered animals[J].Acta Veterinaria et Zootechnica Sinica,2022,53(8):2479-2489. doi: 10.11843/j.issn.0366-6964.2022.08.007
6	VININGL M,ZAKL J,HARVEYS C,et al.The role of apoptosis in cryopreserved animal oocytes and embryos[J].Theriogenology,2021,173,93-101. doi: 10.1016/j.theriogenology.2021.07.017
7	ZHUAN Q, LI J, DU X, et al. Antioxidant procyanidin B2 protects oocytes against cryoinjuries via mitochondria regulated cortical tension. J Anim Sci Biotechnol. 2022;13(1): 95.
8	KEREM,LIUP C,CHENY K,et al.Ultrastructural characterization of porcine growing and in vitro matured oocytes[J].Animals (Basel),2020,10(4):664.
9	CHENJ S,TSAIL K,YEHT Y,et al.Effects of electromagnetic waves on oocyte maturation and embryonic development in pigs[J].J Reprod Dev,2021,67(6):392-401. doi: 10.1262/jrd.2021-074
10	CAOL H,YANGT,HUANGS H,et al.Expression patterns of ZO-1/2 and their effects on porcine oocyte in vitro maturation and early embryonic development[J].Theriogenology,2021,161,262-270. doi: 10.1016/j.theriogenology.2020.12.009
11	ABEDPOURN,SHOOREIH,RAJAEIF.Detrimental effects of vitrification on integrin genes (α9 and β1) and in vitro fertilization in mouse oocytes[J].Mol Biol Rep,2023,50(6):4823-4829. doi: 10.1007/s11033-023-08377-6
12	GONZALEZ-PLAZAA,CAMBRAJ M,PARRILLAI,et al.The open Cryotop system is effective for the simultaneous vitrification of a large number of porcine embryos at different developmental stages[J].Front Vet Sci,2022,9,936753. doi: 10.3389/fvets.2022.936753
13	TAJIMAS,UCHIKURAK,KURITAT,et al.Insemination of recipient sows improves the survival to term of vitrified and warmed porcine expanded blastocysts transferred non-surgically[J].Anim Sci J,2020,91(1):e13453. doi: 10.1111/asj.13453
14	QIUJ,MATSUKAWAK,KOSHIMOTOC,et al.Equilibrium vitrification of mouse embryos at various developmental stages using low concentrations of cryoprotectants[J].J Reprod Dev,2021,67(2):109-114. doi: 10.1262/jrd.2020-152
15	MARTÍNEZ-RODEROI,GARCIA-MARTÍNEZT,ORDÓÑEZ-LEÓNE A,et al.A shorter equilibration period improves post-warming outcomes after vitrification and in straw dilution of in vitro-produced bovine embryos[J].Biology (Basel),2021,10(2):142.
16	SKRZYPEKK,NIBBELINKM C,LIEFERS-VISSERJ,et al.A high cell-bearing capacity multibore hollow fiber device for macroencapsulation of islets of Langerhans[J].Macromol Biosci,2020,20(8):2000021. doi: 10.1002/mabi.202000021
17	ALMIÑANAC,DUBUISSONF,BAUERSACHSS,et al.Unveiling how vitrification affects the porcine blastocyst: clues from a transcriptomic study[J].J Anim Sci Biotechnol,2022,13(1):46. doi: 10.1186/s40104-021-00672-1
18	ALMUBARAKA,LEES,YUI J,et al.Effects of Nobiletin supplementation on the freezing diluent on porcine sperm cryo-survival and subsequent in vitro embryo development[J].Theriogenology,2023,214,314-322.
19	ABDELHADYA W,MITTAN-MOREAUD W,CRANEP L,et al.Ice formation and its elimination in cryopreservation of oocytes[J].Sci Rep,2024,14,18809. doi: 10.1038/s41598-024-69528-8
20	CUELLOC,GONZÁLEZ-PLAZAA,CAMBRAJ M,et al.Vitrification of pig embryos dysregulates the microRNA transcriptome profile[J].Theriogenology,2024,226,243-252. doi: 10.1016/j.theriogenology.2024.06.001
21	NAKAGAWAY,KANEKOT.Improvement of survivability and developmental ability in vitrified rat oocytes[J].Cryobiology,2024,115,104882. doi: 10.1016/j.cryobiol.2024.104882
22	DI GUARDOF,RACCAA,COTICCHIOG,et al.Impact of cell loss after warming of human vitrified day 3 embryos on obstetric outcome in single frozen embryo transfers[J].J Assist Reprod Genet,2022,39(9):2069-2075. doi: 10.1007/s10815-022-02572-3
23	HOCHIS.Cryodevices developed for minimum volume cooling vitrification of bovine oocytes[J].Anim Sci J,2022,93(1):e13683. doi: 10.1111/asj.13683
24	MORADOS,APARICIOA,PINCHETTID,et al.Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming[J].Open Vet J,2023,13(11):1416-1424. doi: 10.5455/OVJ.2023.v13.i11.4
25	MARCANTONINIG,BARTOLINID,ZATINIL,et al.Natural cryoprotective and cytoprotective agents in cryopreservation: a focus on melatonin[J].Molecules,2022,27(10):3254. doi: 10.3390/molecules27103254
26	GONZALEZ-PLAZAA,CAMBRAJ M,GARCIA-CANOVASM,et al.Cryotop vitrification of large batches of pig embryos simultaneously provides excellent postwarming survival rates and minimal interference with gene expression[J].Theriogenology,2023,206,1-10. doi: 10.1016/j.theriogenology.2023.04.011
27	VAN NGUYENT,DOL T K,NGUYENN A T,et al.The effects of an in vitro oocyte maturation system and chlorogenic acid supplementation during embryo culture on the development of porcine cloned embryos derived from native vietnamese ban pigs[J].Vet Med Int,2023,2023(1):5702970. doi: 10.1155/2023/5702970
28	WIESAKT,GORYSZEWSKA-SZCZUREKE.Effect of vitrification on the expression of genes in porcine blastocysts derived from in vitro matured oocytes[J].Syst Biol Reprod Med,2022,68(4):239-246. doi: 10.1080/19396368.2022.2072788
29	RIENZIL,GRACIAC,MAGGIULLIR,et al.Oocyte, embryo and blastocyst cryopreservation in ART: systematic review and meta-analysis comparing slow-freezing versus vitrification to produce evidence for the development of global guidance[J].Hum Reprod Update,2017,23(2):139-155.
30	XIANGD C,JIAB Y,GUOJ X,et al.Transcriptome analysis of mRNAs and long non-coding RNAs during subsequent embryo development of porcine cloned zygotes after vitrification[J].Front Genet,2021,12,753327. doi: 10.3389/fgene.2021.753327
31	SOMFAIT,HARAGUCHIS,DANG-NGUYENT Q,et al.Vitrification of porcine immature oocytes and zygotes results in different levels of DNA damage which reflects developmental competence to the blastocyst stage[J].PLoS One,2023,18(3):e0282959. doi: 10.1371/journal.pone.0282959
32	GOMISJ,CUELLOC,SANCHEZ-OSORIOJ,et al.Forskolin improves the cryosurvival of in vivo-derived porcine embryos at very early stages using two vitrification methods[J].Cryobiology,2013,66(2):144-150. doi: 10.1016/j.cryobiol.2012.12.009
33	XUH X,WANGX G,TAOR X,et al.Optimal stage for cryotop vitrification of porcine embryos[J].Cell Reprogram,2022,24(3):132-141. doi: 10.1089/cell.2022.0001
34	USHIJIMAH,YOSHIOKAH,ESAKIR,et al.Improved survival of vitrified in vivo-derived porcine embryos[J].J Reprod Dev,2004,50(4):481-486. doi: 10.1262/jrd.50.481
35	HOCHIS,IDEM,UENOS,et al.High survival of bovine mature oocytes after nylon mesh vitrification, as assessed by intracytoplasmic sperm injection[J].J Reprod Dev,2022,68(5):335-339. doi: 10.1262/jrd.2022-053
36	CUELLOC,MARTINEZC A,CAMBRAJ M,et al.Effects of vitrification on the blastocyst gene expression profile in a porcine model[J].Int J Mol Sci,2021,22(3):1222. doi: 10.3390/ijms22031222
37	TUC F,PENGS H,CHUANGC K,et al.Reproductive technologies needed for the generation of precise gene-edited pigs in the pathways from laboratory to farm[J].Anim Biosci,2023,36(2):339-349. doi: 10.5713/ab.22.0389
38	LÓPEZA,BETANCOURTM,DUCOLOMBY,et al.DNA damage in cumulus cells generated after the vitrification of in vitro matured porcine oocytes and its impact on fertilization and embryo development[J].Porc Health Manag,2021,7(1):56. doi: 10.1186/s40813-021-00235-w
39	KAWAKAMIM,KATOY,TSUNODAY.The effects of time of first cleavage, developmental stage, and delipidation of nuclear-transferred porcine blastocysts on survival following vitrification[J].Anim Reprod Sci,2008,106(3-4):402-411. doi: 10.1016/j.anireprosci.2007.06.002
40	GAOM,CHENM J,CHENQ Z,et al.Integration of parallel metabolomics and transcriptomics reveals metabolic patterns in porcine oocytes during maturation[J].Front Endocrinol (Lausanne),2023,14,1131256. doi: 10.3389/fendo.2023.1131256
41	MARCO-JIMÉNEZF,GARCIA-DOMINGUEZX,GARCÍA-VALEROL,et al.A 3D-Printed Large Holding Capacity Device for Minimum Volume Cooling Vitrification of Embryos in Prolific Livestock Species[J].Animals (Basel),2023,13(5):791.
42	HÖLKERM,PETERSENB,HASSELP,et al.Duration of in vitro maturation of recipient oocytes affects blastocyst development of cloned porcine embryos[J].Cloning Stem Cells.,2005,7(1):35-44. doi: 10.1089/clo.2005.7.35
43	TAJIMAS,MOTOYAMAS,WAKIYAY,et al.Piglet production by non-surgical transfer of vitrified embryos, transported to commercial swine farms and warmed on site[J].Anim Sci J,2021,Dec92(1):e13588. doi: 10.1111/asj.13588
44	NGUYENV K,VUH,NGUYENH T T,et al.Comparison of the microdrop and minimum volume cooling methods for vitrification of porcine in vitro-produced zygotes and blastocysts after equilibration in low concentrations of cryoprotectant agents[J].J Reprod Dev,2018,64(5):457-462. doi: 10.1262/jrd.2018-047
45	NAJAFZADEHV,SECHERJ B M,PIHLM,et al.Vitrification yields higher cryo-survival rate than slow freezing in biopsied bovine in vitro produced blastocysts[J].Theriogenology,2021,171,44-54. doi: 10.1016/j.theriogenology.2021.04.020
46	KAJDASZA,WARZYCHE,DEREBECKAN,et al.Lipid stores and lipid metabolism associated gene expression in porcine and bovine parthenogenetic embryos revealed by fluorescent staining and RNA-seq[J].Int J Mol Sci,2020,21(18):6488. doi: 10.3390/ijms21186488
47	IBAYASHIM,AIZAWAR,MITSUIJ,et al.Homeostatic regulation of lipid droplet content in mammalian oocytes and embryos[J].Reproduction,2021,162(6):R99-R109. doi: 10.1530/REP-21-0238
48	DUNNINGK R,RUSSELLD L,ROBKERR L.Lipids and oocyte developmental competence: the role of fatty acids and β-oxidation[J].Reproduction,2014,148(1):R15-R27. doi: 10.1530/REP-13-0251
49	WANGC,NIUY,CHID,et al.Influence of delipation on the energy metabolism in pig parthenogenetically activated embryos[J].Reprod Domest Anim,2015,50(5):826-833. doi: 10.1111/rda.12596
50	RAZAS H A,ABD EL-AZIZA H,ABDELNOURS A,et al.The role of forskolin as a lipolytic stimulator during in vitro oocyte maturation and the in vitro embryo production of livestock[J].Reprod Domest Anim,2021,56(12):1486-1496. doi: 10.1111/rda.14021
51	AMSTISLAVSKYS,MOKROUSOVAV,BRUSENTSEVE,et al.Influence of cellular lipids on cryopreservation of mammalian oocytes and preimplantation embryos: a review[J].Biopreserv Biobank,2019,17(1):76-83. doi: 10.1089/bio.2018.0039
52	CASTILLO-MARTÍNM,BONETS,MORATÓR,et al.Supplementing culture and vitrification-warming media with L-ascorbic acid enhances survival rates and redox status of IVP porcine blastocysts via induction of GPX1 and SOD1 expression[J].Cryobiology,2014,68(3):451-458. doi: 10.1016/j.cryobiol.2014.03.001
53	LIJ,XIONGS,ZHAOY,et al.Effect of the re-vitrification of embryos at different stages on embryonic developmental potential[J].Front Endocrinol (Lausanne),2021,12,653310. doi: 10.3389/fendo.2021.653310
54	MENEZOY,CLEMENTP,DALEB,et al.Modulating oxidative stress and epigenetic homeostasis in preimplantation IVF embryos[J].Zygote,2022,30(2):149-158. doi: 10.1017/S0967199421000356
55	MENEZOY,ELDERK,CLEMENTP,et al.Biochemical hazards during three phases of assisted reproductive technology: repercussions associated with epigenesis and imprinting[J].Int J Mol Sci,2022,23(16):8916. doi: 10.3390/ijms23168916
56	PAVANELIA P P,RECUEROS,CHAVESB R,et al.The presence of seminal plasma during liquid storage of pig spermatozoa at 17 ℃ modulates their ability to elicit in vitro capacitation and trigger acrosomal exocytosis[J].Int J Mol Sci,2020,21(12):4520. doi: 10.3390/ijms21124520
57	HARDYM L M,DAYM L,MORRISM B.Redox regulation and oxidative stress in mammalian oocytes and embryos developed in vivo and in vitro[J].Int J Environ Res Public Health,2021,18(21):11374. doi: 10.3390/ijerph182111374
58	SOTO-HERASS,PARAMIOM T.Impact of oxidative stress on oocyte competence for in vitro embryo production programs[J].Res Vet Sci,2020,132,342-350. doi: 10.1016/j.rvsc.2020.07.013
59	JIANGY,HEY T,PANX C,et al.Advances in oocyte maturation in vivo and in vitro in mammals[J].Int J Mol Sci,2023,24(10):9059. doi: 10.3390/ijms24109059
60	NIUY J,ZHOUW J,NIEZ W,et al.Melatonin enhances mitochondrial biogenesis and protects against rotenone-induced mitochondrial deficiency in early porcine embryos[J].J Pineal Res,2020,68(2):e12627. doi: 10.1111/jpi.12627
61	SUNM H,JIANGW J,LIX H,et al.ATF6 aggravates apoptosis in early porcine embryonic development by regulating organelle homeostasis under high-temperature conditions[J].Zool Res,2023,44(5):848-859. doi: 10.24272/j.issn.2095-8137.2023.080
62	KAGEYAMAM,ITOJ,SHIRASUNAK,et al.Mitochondrial reactive oxygen species regulate mitochondrial biogenesis in porcine embryos[J].J Reprod Dev,2021,67(2):141-147. doi: 10.1262/jrd.2020-111
63	YANGS G,BAEJ W,PARKH J,et al.Mito-TEMPO protects preimplantation porcine embryos against mitochondrial fission-driven apoptosis through DRP1/PINK1-mediated mitophagy[J].Life Sci,2023,315,121333. doi: 10.1016/j.lfs.2022.121333
64	LIUR P,WANGJ,WANGX Q,et al.Xanthoangelol promotes early embryonic development of porcine embryos by relieving endoplasmic reticulum stress and enhancing mitochondrial function[J].Reprod BioMed Online,2023,47(2):103211. doi: 10.1016/j.rbmo.2023.04.002
65	ZHOUD J,SUNM H,LEES H,et al.ROMO1 is required for mitochondrial metabolism during preimplantation embryo development in pigs[J].Cell Div,2021,16(1):7. doi: 10.1186/s13008-021-00076-7
66	WANGC R,JIH W,HES Y,et al.Chrysoeriol improves in vitro porcine embryo development by reducing oxidative stress and autophagy[J].Vet Sci,2023,10(2):143. doi: 10.3390/vetsci10020143
67	NIUY J,WANGC F,XIONGQ,et al.Distribution and content of lipid droplets and mitochondria in pig parthenogenetically activated embryos after delipation[J].Theriogenology,2015,83(1):131-138. doi: 10.1016/j.theriogenology.2014.09.002
68	TOSTIE,MÉNÉZOY.Gamete activation: basic knowledge and clinical applications[J].Hum Reprod Update,2016,22(4):420-439. doi: 10.1093/humupd/dmw014
69	WASSARMANP M,LITSCHERE S.Zona pellucida genes and proteins: essential players in mammalian oogenesis and fertility[J].Genes (Basel),2021,12(8):1266. doi: 10.3390/genes12081266
70	NGUYENN T,HIRATAM,TANIHARAF,et al.In vitro development of zona pellucida-free porcine zygotes cultured individually after vitrification[J].Cryo Letters,2020,41(2):86-91.
71	SCIORIOR,MANNAC,FAUQUEP,et al.Can cryopreservation in Assisted Reproductive Technology (ART) induce epigenetic changes to gametes and embryos?[J].J Clin Med,2023,12(13):4444. doi: 10.3390/jcm12134444
72	ESTUDILLOE,JIMÉNEZA,BUSTAMANTE-NIEVESP E,et al.Cryopreservation of gametes and embryos and their molecular changes[J].Int J Mol Sci,2021,22(19):10864. doi: 10.3390/ijms221910864
73	TIRGARP,SARMADIF,NAJAFIM,et al.Toward embryo cryopreservation-on-a-chip: a standalone microfluidic platform for gradual loading of cryoprotectants to minimize cryoinjuries[J].Biomicrofluidics,2021,15(3):034104. doi: 10.1063/5.0047185
74	ZHANGL,QIX,NINGW,et al.Single-cell transcriptome profiling revealed that vitrification of somatic cloned porcine blastocysts causes substantial perturbations in gene expression[J].Front Genet,2020,11,640. doi: 10.3389/fgene.2020.00640
75	PEROM E,ZULLOG,ESPOSITOL,et al.Inhibition of apoptosis by caspase inhibitor Z-VAD-FMK improves cryotolerance of in vitro derived bovine embryos[J].Theriogenology,2018,108,127-135. doi: 10.1016/j.theriogenology.2017.11.031
76	DENGD M,XIEJ,TIANY,et al.Effects of meiotic stage-specific oocyte vitrification on mouse oocyte quality and developmental competence[J].Front Endocrinol (Lausanne),2023,14,1200051. doi: 10.3389/fendo.2023.1200051
77	TAJIMAS,MOTOYAMAS,WAKIYAY,et al.Piglet production by non-surgical transfer of vitrified embryos, transported to commercial swine farms and warmed on site[J].Anim Sci J,2020,91(1):e13476. doi: 10.1111/asj.13476
78	LEEK,UHK,FARRELLK.Current progress of genome editing in livestock[J].Theriogenology,2020,150,229-235. doi: 10.1016/j.theriogenology.2020.01.036
79	CHENP R,REDELB K,KERNSK C,et al.Challenges and considerations during in vitro production of porcine embryos[J].Cells,2021,10(10):2770. doi: 10.3390/cells10102770
80	HARAK,ABEEY,KUMADAN,et al.Extrusion and removal of lipid from the cytoplasm of porcine oocytes at the germinal vesicle stage: centrifugation under hypertonic conditions influences vitrification[J].Cryobiology,2005,50(2):216-222. doi: 10.1016/j.cryobiol.2005.01.003
81	OECKLJ,BAST-HABERSBRUNNERA,FROMMET,et al.Isolation, culture, and functional analysis of murine thermogenic adipocytes[J].STAR Protoc,2020,1(3):100118. doi: 10.1016/j.xpro.2020.100118
82	TATSUMIT,TAKAYAMAK,ISHⅡS,et al.Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse[J].Development,2018,145(4):dev161893. doi: 10.1242/dev.161893
83	TIBBOA J,MIKAD,DOBIS,et al.Phosphodiesterase type 4 anchoring regulates cAMP signaling to Popeye domain-containing proteins[J].J Mol Cell Cardiol,2022,165,86-102. doi: 10.1016/j.yjmcc.2022.01.001
84	ISAT,SOMFAIT,OYADOMARIM,et al.Production of Agu piglets after transfer of embryos produced in vitro[J].Anim Sci J,2022,93(1):e13685. doi: 10.1111/asj.13685
85	TATSUMIT,TAKAYAMAK,ISHⅡS,et al.Forced lipophagy reveals that lipid droplets are required for early embryonicdevelopment in mouse[J].Development,2018,145(4):dev161893. doi: 10.1242/dev.161893
86	EL-SOKARYM M M,EL-NABYA A H,HAMEEDA R A E,et al.Impact of L-carnitine supplementation on the in vitro developmental competence and cryotolerance of buffalo embryos[J].Vet World,2021,14(12):3164-3169.
87	RAKHMANOVAT,MOKROUSOVAV,OKOTRUBS,et al.Effects of forskolin on cryopreservation and embryo development in the domestic cat[J].Theriogenology,2023,210,192-198. doi: 10.1016/j.theriogenology.2023.07.035
88	NAMULAZ,HIRATAM,LEQ A,et al.Zona pellucida treatment before CRISPR/Cas9-mediated genome editing of porcine zygotes[J].Vet Med Sci,2022,8(1):164-169. doi: 10.1002/vms3.659
89	ROMEKM,GAJDAB,KRZYSZTOFOWICZE,et al.New technique to quantify the lipid composition of lipid droplets in porcine oocytes and pre-implantation embryos using Nile Red fluorescent probe[J].Theriogenology,2011,75(1):42-54. doi: 10.1016/j.theriogenology.2010.06.040
90	CAMBRAJ M,GILM A,CUELLOC,et al.Cytokine profile in peripheral blood mononuclear cells differs between embryo donor and potential recipient sows[J].Front Vet Sci,2024,11,1333941. doi: 10.3389/fvets.2024.1333941
91	FUX W,WUG Q,LIJ J,et al.Positive effects of Forskolin (stimulator of lipolysis) treatment on cryosurvival of in vitro matured porcine oocytes[J].Theriogenology,2011,75(2):268-275. doi: 10.1016/j.theriogenology.2010.08.013
92	YANGC X,LIANGH,WUZ W,et al.Identification of lncRNAs involved in maternal-to-zygotic transition of in vitro-produced porcine embryos by single-cell RNA-seq[J].Reprod Domest Anim.,2022,57(1):111-122. doi: 10.1111/rda.14034
93	ZHUANQ,MAH J,CHENJ,et al.Cytoplasm lipids can be modulated through hormone-sensitive lipase and are related to mitochondrial function in porcine IVM oocytes[J].Reprod Fertil Dev,2020,32(7):667-675. doi: 10.1071/RD19047
94	LIJ Y,XIONGS,ZHAOY H,et al.Effect of the re-vitrification of embryos at different stages on embryonic developmental potential[J].Front Endocrinol (Lausanne),2021,12,653310. doi: 10.3389/fendo.2021.653310
95	CUELLOC,MARTINEZC A,NOHALEZA,et al.Effective vitrification and warming of porcine embryos using a pH-stable, chemically defined medium[J].Sci Rep,2016,6,33915. doi: 10.1038/srep33915
96	SANCHEZ-OSORIOJ,CUELLOC,GILM A,et al.Vitrification and warming of in vivo-derived porcine embryos in a chemically defined medium[J].Theriogenology,2010,73(3):300-308. doi: 10.1016/j.theriogenology.2009.07.031
97	MARTINEZC A,CUELLOC,PARRILLAI,et al.Exogenous melatonin in the culture medium does not affect the development of in vivo-derived pig embryos but substantially improves the quality of in vitro-produced embryos[J].Antioxidants (Basel),2022,11(6):1177. doi: 10.3390/antiox11061177
98	CUELLOC,MARTINEZC A,CAMBRAJ M,et al.Vitrification effects on the transcriptome of in vivo-derived porcine morulae[J].Front Vet Sci,2021,8,771996. doi: 10.3389/fvets.2021.771996
99	XIANGD C,JIAB Y,QUANG B,et al.Effect of knockout serum replacement during postwarming recovery culture on the development and quality of vitrified parthenogenetic porcine blastocysts[J].Biopreserv Biobank,2019,17(4):342-351. doi: 10.1089/bio.2018.0132
100	BUDANIM C,TIBONIG M.Effects of supplementation with natural antioxidants on oocytes and preimplantation embryos[J].Antioxidants (Basel),2020,9(7):612. doi: 10.3390/antiox9070612
101	GAOL,DUM,ZHUANQ,et al.Melatonin rescues the aneuploidy in mice vitrified oocytes by regulating mitochondrial heat product[J].Cryobiology,2019,89,68-75. doi: 10.1016/j.cryobiol.2019.05.005
102	YOONS Y,EUMJ H,CHAS K,et al.Prematuration culture with phosphodiesterase inhibitors after vitrification may induce recovery of mitochondrial activity in vitrified mouse immature oocytes[J].Biopreserv Biobank,2018,16(4):296-303. doi: 10.1089/bio.2018.0010
103	PANB,QAZII H,GUOS C,et al.Melatonin improves the first cleavage of parthenogenetic embryos from vitrified-warmed mouse oocytes potentially by promoting cell cycle progression[J].J Anim Sci Biotechnol,2021,12(1):84. doi: 10.1186/s40104-021-00605-y
104	LINQ Y,LEQ A,TAKEBAYASHIK,et al.Short-term preservation of porcine zygotes at ambient temperature using a chemically defined medium[J].Anim Sci J,2022,93(1):e13711. doi: 10.1111/asj.13711
105	KIKUCHIK,KASHIWAZAKIN,NAGAIT,et al.Selected aspects of advanced porcine reproductive technology[J].Reprod Domest Anim,2008,43(S2):401-406. doi: 10.1111/j.1439-0531.2008.01191.x
106	WHALEYD,DAMYARK,WITEKR P,et al.Cryopreservation: an overview of principles and cell-specific considerations[J].Cell Transplant,2021,30,963689721999617. doi: 10.1177/0963689721999617
107	CZERNIKM,WINIARCZYKD,SAMPINOS,et al.Author correction: mitochondrial function and intracellular distribution is severely affected in in vitro cultured mouse embryos[J].Sci Rep,2022,12(1):21276. doi: 10.1038/s41598-022-25820-z
108	NGUYENH T,NGUYENN T,NGUYENL V,et al.The effects of pretreatment with Cyclosporin A and Docetaxel before vitrification of porcine immature oocytes on subsequent embryo development[J].Reprod Biol,2023,23(4):100798. doi: 10.1016/j.repbio.2023.100798
109	DUARTEF V,CIAMPID,DUARTEC B.Mitochondria as central hubs in synaptic modulation[J].Cell Mol Life Sci,2023,80(6):173. doi: 10.1007/s00018-023-04814-8
110	FUJINOY,KOJIMAT,NAKAMURAY,et al.Metal mesh vitrification (MMV) method for cryopreservation of porcine embryos[J].Theriogenology,2008,70(5):809-817. doi: 10.1016/j.theriogenology.2008.05.045
111	PANGY W,ANL,WANGP,et al.Treatment of porcine donor cells and reconstructed embryos with the antioxidant melatonin enhances cloning efficiency[J].J Pineal Res.,2013,54(4):389-397. doi: 10.1111/jpi.12024
112	JOES Y,YANGS G,LEEJ H,et al.Stabilization of F-actin cytoskeleton by paclitaxel improves the blastocyst developmental competence through P38 MAPK activity in porcine embryos[J].Biomedicines,2022,10(8):1867. doi: 10.3390/biomedicines10081867
113	PARRILLAI,GILM A,CUELLOC,et al.Immunological uterine response to pig embryos before and during implantation[J].Reprod Domest Anim,2022,57(S5):4-13. doi: 10.1111/rda.14142

物种 Species	评价指标 Evaluation indicator	新鲜胚胎/% Fresh embryo	冷冻胚胎/% Frozen embryo	参考文献 Reference
牛Cattle	妊娠率	51.35	35.89	[11]
猪Pig	囊胚腔恢复率	100.00	31.03	[12]
猪Pig	孵化率	90.90	66.70	[13]
羊Sheep	孵化率	91.89	77.50	[14]
羊Lamb	妊娠率	54.30	5.00	[15]
鼠Mouse	孵化率	92.90	89.40	[16]

胚胎阶段 Embryonic stage	玻璃化方法 Vitrification freezing	存活率/% Survival rate	囊胚率/% Blastocyst rate	孵化率/% Hatchability	参考文献 Reference
受精卵 Zygote	MD	98.5±0.7	21.1±1.5		[29]
	MVC	90.1±5.8	19.1±4.1		[30]
	SSV	93.4±63.5	15.8±62.1
2细胞	SOPS		66.1±6.0	4.9±3.4	[31]
2-Cell	SSV		76.5±5.2	3.6±2.5
4细胞	SOPS		53.8±6.9	9.1±6.2
4-Cell	SSV		34.5±6.3	5.0±5.0
桑葚胚 Morula	SOPS		94.6±3.0	58.5±6.8	[32]
	SSV		96.6±2.3	48.3±6.6	[32]
	MVC	95	74		[33]
	HFV		73.8	32.3	[22]
	HFV		77.6	10.6	[34]
	HFV		90.3	40.9	[35]
	MVC		31.5		[36]
囊胚 Blastocyst	SOPS	81.0±3.7		46.2±0.5	[37]
	MVAC	85.0	45.0		[38]
	Cryotop	79±6	21±6		[39]
	MD	26.8±2.7		2.5±2.5	[9]
	MVC	52.2±6.1		2.3±1.6	[9]
膨大囊胚 Expanded blastocyst	OPS	86.2		74.1	[40]
	MMV	84.4			[41]
	MVAC	88.9±3.9		69.4±7.3	[42]

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Advances in Cryopreservation of Porcine Embryo

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