牛体外胚胎冷冻保存的研究进展

doi:10.11843/j.issn.0366-6964.2023.02.004

Abstract

Abstract: In vitro embryo cryopreservation technology is an important part of embryo transfer technology, which plays an important role in assisted reproduction technology, and it is also important for germplasm resource conservation, enhancing genetic improvement and promoting international circulation of high-quality germplasm. However, the high lipid content, elevated levels of reactive oxygen species and mechanical damage in in vitro embryo cryopreservation lead to low efficiency of in vitro embryo cryopreservation, which greatly limits the wide application of in vitro embryo cryopreservation technology. Numerous studies have shown that the survival rate and developmental capacity of frozen embryos can be effectively improved by means of lipid removal, optimization of in vitro embryo culture medium, artificial collapse of blastocyst cavities and optimization of freezing procedures. Therefore, this paper outlines the research progress of in vitro embryo cryopreservation technology and the problems in cryopreservation process, and summarizes the methods that can improve the efficiency of in vitro embryo cryopreservation, aiming to provide the reference for improving the efficiency of in vitro embryo cryopreservation.

Key words: cattle, in vitro embryo, cryopreservation, freezing efficiency

CLC Number:

S823.3

FENG Xiaoyi, XU Xi, ZHANG Hang, YANG Baigao, ZHANG Peipei, HAO Haisheng, DU Weihua, ZHU Huabin, CUI Kai, ZHAO Xueming. Advances in Cryopreservation of Bovine Embryo in vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 451-462.

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Advances in Cryopreservation of Bovine Embryo in vitro

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