2017—2023年我国猪圆环病毒3型的遗传变异分析

doi:10.11843/j.issn.0366-6964.2025.06.029

Abstract

Abstract:

The aim of this study was to understand the molecular epidemiological characteristics and genetic variation of porcine circovirus type 3 (PCV3) in China in recent years. In this study, 168 domestic (collected from 2017 to 2023) and 9 foreign PCV3 genome sequences were selected from GenBank database, and their phylogenetic tree construction, nucleotide homology comparison, amino acid sequence comparison, epitope prediction and gene recombination were analyzed by bioinformatics software, in order to clarify the transmission and genetic evolution law of PCV3 in China. The results showed that the genotypes of PCV3 in China can be divided into two subtypes, namely, PCV3a and PCV3b, and both of them can be further divided into different gene clusters: PCV3a-1~PCV3a-3 and PCV3b-1~PCV3b-5, among which PCV3a-1 and PCV3b-2 have the largest number and the highest proportion. The whole genome sequence similarity of 177 PCV3 is between 98.3% and 100%, and there are three main point mutation regions in the amino acid sequence encoded by ORF2 gene, without base deletion and insertion. In addition, the analysis of gene recombination shows that there is only one possible recombination event between these whole genome sequences of PCV3, which indicates that the prevalent genotypes of PCV3 in China are in a relatively stable stage. This study found a genotyping method that is likely to be widely recognized in the future through strict standard definition, and based on this genotyping method, the results obtained when analyzing the genetic variation of PCV3 in China from 2017 to 2023 are reliable, which not only provides a unified framework for the future research on genetic variation of PCV3, but also provides basic data for further revealing the molecular epidemiological characteristics, genetic diversity, vaccine design and prevention and control strategies of PCV3.

Key words: porcine circovirus type 3, genetic evolution analysis, phylogenetic tree, reorganization analysis

CLC Number:

S852.65

WANG Yan, HUANG Xinyu, WU Guiying, WU Wanping, Lü Qizhuang. Genetic Variation Analysis of Porcine Circovirus Type 3 in China from 2017 to 2023[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(6): 2857-2867.

Figures/Tables 7

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References 32

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年份 Year	不同亚型检测率/% Detection rate of different gene subtype
年份 Year	3a-1	3a-2	3a-3	3b-1	3b-2	3b-3	3b-4	3b-5
2017	12.31	30.77	1.54	1.54	1.54	30.77	6.15	15.38
2018	21.95	31.71	2.44	4.88	4.88	17.07	9.76	9.76
2019	23.53	41.18	5.88	0.00	17.65	0.00	0.00	11.76
2020	23.08	7.70	0.00	0.00	69.23	0.00	0.00	0.00
2021	12.50	25.00	0.00	12.50	0.00	25.00	0.00	25.00
2022	0.00	0.00	0.00	0.00	20.00	0.00	20.00	60.00
2023	73.68	0.00	0.00	0.00	0.00	21.05	0.00	5.26
总计 Total	22.62	25.60	1.79	23.81	9.52	19.64	5.36	13.10

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Genetic Variation Analysis of Porcine Circovirus Type 3 in China from 2017 to 2023

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