四川盆地6个地方鸡群体遗传关系研究

doi:10.11843/j.issn.0366-6964.2022.02.005

Abstract

Abstract: With the convenience of transportation and market-oriented cross utilization, the genetic resources of local chickens in China are facing the problems of blood mixing and the unclear breed source and genetic relationship among breeds. The study aimed to provide a basis for the Third National Census of Livestock and Poultry Genetic Resources. A simplified genomic GBS sequencing was carried out on 118 individuals from 6 local chicken populations (10 males and 10 females in each population) located in Daba Mountains and Wumeng Mountains in China. The nucleotide polymorphism (Pi), observed heterozygosity (Ho), expected heterozygosity (He), polymorphic information content (PIC), number of observed alleles (Na), number of effective alleles (Ne), genetic differentiation (Fst), genetic distance (DR) and gene flow (Nm) of these populations were analyzed. It was found that the genetic diversity of black feather population of Yanshui chicken was low (He=0.154; PIC=0.125), and the genetic diversity of Daninghe chicken was the most abundant (He=0.197, PIC=0.162). The genetic differentiation between Chengkou mountain chicken and Daninghe chicken was the lowest (Fst =0.053), the gene flow was the highest (Nm=4.50), the genetic differentiation between Fengyan black bone chicken and black feather population of Yanshui chicken was the highest (Fst=0.183), and the corresponding gene flow was the lowest (Nm=1.12). Phylogenetic tree and principal component analysis showed that the white feather population and black feather population of Yanshui chicken were clustered into one class. Chengkou mountain chicken, Daninghe chicken and Jiuyuan black chicken were clustered into one class, while Fengyan black bone chicken was a separate class. Structure analysis showed that the blood of Chengkou mountain chicken and white feather population of Yanshui chicken was mixed. This study proved the genetic relationship of local breeds in adjacent areas. Fengyan black bone chicken located in Wumeng Mountain has a long genetic distance from Jiuyuan black chicken, Chengkou mountain chicken, Daninghe chicken, black feather and white feather populations of Yanshui chicken in Daba Mountain. This study provides a basis for the protection, development and utilization of local chicken breeds.

Key words: local chicken breeds, genetic diversity, simplified genome, gene flow, phylogenetic tree

CLC Number:

S831.2

YOU Guishuang, YI Xingyou, JIANG Meishan, LI Zhongwei, TAN Helin, CAO Chi, SHU Gang, TIAN Yaofu, ZHU Qing, ZHAO Xiaoling. Study on the Genetic Relationship of Six Local Chicken Populations from Sichuan Basin[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 370-380.

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Study on the Genetic Relationship of Six Local Chicken Populations from Sichuan Basin

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