猪流产胎儿中猪圆环病毒3型的检测及其遗传演化分析

doi:10.11843/j.issn.0366-6964.2022.05.020

Abstract

Abstract: The study aimed to explore the prevalence and genetic evolution of porcine circovirus type 3 (PCV3) in aborted fetuses. In this study, PCV3 detection, amplification and sequencing were performed on 680 samples of porcine abortion fetus in Hunan Province from 2015 to 2017, and the obtained sequences were analyzed by bioinformatics software for genetic evolution. The results showed that the positive rate of PCV3 in fetal samples of porcine abortion was 24.4% (166/680), and the positive rates from 2015 to 2017 were 18.2% (26/143), 22.7% (54/238) and 28.8% (86/299), respectively. A full-length genomic sequence of PCV3 was obtained from PCV3 positive samples, which was named PCV3/CN/Hunan-57 (GenBank accession No. MZ934695). Compared with the genome sequences of PCV3 strains at home and abroad, the homology was 97.8%-99.2%. The ORF2 gene sequences of five PCV3 strains were obtained, and their nucleotide and amino acid homology were 97.5%-98.3% and 96.7%-99.7%, respectively. Compared with the ORF2 gene sequences of reference strains in China and abroad, their nucleotide and amino acid homology were 96.3%-99.2% and 96.8%-100.0%, respectively. The genetic evolution tree was constructed based on the ORF2 gene sequence and multiple sequence alignment was performed. The obtained positive sequence belonged to PCV3a (1 strain) and PCV3c (4 strains), respectively, and the mutation sites of T100A, G113S and A184S were identified. This study showed that the infection rate of PCV3 in porcine aborted fetuses was high. There were different types of PCV3 infection in porcine aborted fetuses, which provided important reference information for further study of PCV3 genetic variation and its pathogenicity.

Key words: porcine circovirus type 3, genome, Cap, genetic evolution

CLC Number:

S852.659.2

HE Qing, LI Zhoumian, WANG Huan, LI Dantong, TAN Qinghui, ZOU Yawen, SU Jianming, DENG Zhibang, YANG Yi, WANG Naidong. Detection and Genetic Evolution of Porcine Circovirus Type 3 in Swine Aborted Fetus[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1527-1535.

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Detection and Genetic Evolution of Porcine Circovirus Type 3 in Swine Aborted Fetus

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