一株新型鸭呼肠孤病毒的分离鉴定及其σC基因序列分析

doi:10.11843/j.issn.0366-6964.2025.05.046

Abstract

Abstract:

The aim of this study was to investigate the epidemiological status and genetic variation of novel duck reovirus (NDRV) in the Anhui region. A strain of NDRV was isolated from a flock of muscovy ducks exhibiting splenic infarction at a farm in Anhui Province. The strain was designated as AH812. Identification was conducted through RT-PCR, duck embryo isolation, pathogenicity assays, and σC gene sequence analysis. The results indicated that the isolated virus was confirmed as NDRV. Purity tests showed negative results for other common pathogens, including duck hepatitis virus, duck Tembusu virus, Muscovy duck parvovirus, adenovirus, and duck circovirus.Pathogenicity tests were performed by intramuscular injection of 0.5 mL of the AH812 isolate (TCID50=10^-4.25/0.1 mL) into ducklings leg muscle. The inoculated ducklings exhibited typical symptoms, such as splenomegaly and hemorrhage. Sequence analysis revealed that the AH812 strain shares the closest phylogenetic relationship with the 2020 NDRV isolate N7 from Shandong Province (GenBank accession number: MW030529.1).In conclusion, this study successfully isolated a novel NDRV strain, AH812, which demonstrates significant pathogenicity in muscovy ducks.

Key words: novel duck reovirus, isolation, identification, σC gene, genetic evolution analysis

CLC Number:

S852.659.4

LI Ting, ZHANG Chengcheng, WANG Xiuling, ZHANG Xiaorong, WU Yantao. Isolation and Identification of a Novel Duck Reovirus Strain and the Sequence Analysis of Its σC Gene[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(5): 2520-2524.

Figures/Tables 3

References 16

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Isolation and Identification of a Novel Duck Reovirus Strain and the Sequence Analysis of Its σC Gene

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