吉林省肉牛冠状病毒感染状况调查及分析

doi:10.11843/j.issn.0366-6964.2023.02.024

Abstract

Abstract: In order to comprehensively understand the epidemiological situation of bovine coronavirus (BCoV) in beef cattle herds in Jilin Province, blood, nasal swabs, fecal swabs and tissue organs of clinically diseased and dead cattle were collected in different seasons from 12 counties and cities in the east, central and western regions of Jilin Province, using serological and molecular diagnostic testing techniques to conduct an epidemiological investigation of BCoV in the The epidemiological situation of BCoV in some areas of Jilin Province. A total of 1 298 clinical serum samples, 462 clinical samples (including fecal samples, liver, lung, spleen, trachea and other tissue samples) were collected, and PCR detection of clinical samples was performed by applying commercial BCoV antibody detection kits to detect serum antibodies and a novel detection technique of nano-PCR, and sequencing and analysis of positive results detected by nucleic acid. The results showed that the serum positive rate of BCoV antibodies was 1.08%, and the positive rate of clinical samples such as feces and liver was 21.10%. The BCoV prevalent strain in the investigated area was more than 99% homologous to the prevalent strain in Sichuan, China, after sequencing analysis. This study provides a comprehensive survey of BCoV prevalence in central Jilin Province, which enriches the epidemiological survey data of bovine coronavirus and lays the foundation for guiding the prevention and control of bovine coronavirus.

Key words: bovine coronavirus, epidemiological investigation

CLC Number:

S852.659.6

SUN Feiyan, YE Jingfei, WEI Yu, WANG Zixian, ZHANG Jinyu, BING Liyuan, MENG Tingting, WANG Shuai, ZHAO Lifeng, SUN Liang, GUO Li. Epidemiological Investigation and Analysis of Bovine Coronavirus in Beef Cattle in Jilin Province[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 673-682.

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Epidemiological Investigation and Analysis of Bovine Coronavirus in Beef Cattle in Jilin Province

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